Experiment: Trisodium citrate (C); with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS02960 and PFLU_RS02965 are separated by 119 nucleotides PFLU_RS02965 and PFLU_RS02970 overlap by 4 nucleotides PFLU_RS02970 and PFLU_RS02975 are separated by 38 nucleotides
PFLU_RS02960: PFLU_RS02960 - hypothetical protein, at 682,283 to 682,531
_RS02960
PFLU_RS02965: PFLU_RS02965 - cobalt-precorrin-6A reductase, at 682,651 to 683,370
_RS02965
PFLU_RS02970: PFLU_RS02970 - bifunctional cobalt-precorrin-7 (C(5))-methyltransferase/cobalt-precorrin-6B (C(15))-methyltransferase, at 683,367 to 684,572
_RS02970
PFLU_RS02975: PFLU_RS02975 - precorrin-3B synthase, at 684,611 to 685,957
_RS02975
Position (kb) 683
684
685 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 682.544 kb on - strand at 682.571 kb on + strand at 682.571 kb on + strand at 682.586 kb on + strand at 682.586 kb on + strand at 682.586 kb on + strand at 682.586 kb on + strand at 682.586 kb on + strand at 682.586 kb on + strand at 682.587 kb on - strand at 682.587 kb on - strand at 682.587 kb on - strand at 682.587 kb on - strand at 682.587 kb on - strand at 682.587 kb on - strand at 682.587 kb on - strand at 682.587 kb on - strand at 682.588 kb on + strand at 682.588 kb on + strand at 682.588 kb on + strand at 682.588 kb on + strand at 682.588 kb on + strand at 682.588 kb on + strand at 682.588 kb on + strand at 682.588 kb on + strand at 682.588 kb on + strand at 682.588 kb on + strand at 682.588 kb on + strand at 682.588 kb on + strand at 682.588 kb on + strand at 682.588 kb on + strand at 682.589 kb on - strand at 682.589 kb on - strand at 682.589 kb on - strand at 682.589 kb on - strand at 682.589 kb on - strand at 682.589 kb on - strand at 682.589 kb on - strand at 682.589 kb on - strand at 682.589 kb on - strand at 682.589 kb on - strand at 682.589 kb on - strand at 682.605 kb on + strand at 682.605 kb on + strand at 682.605 kb on + strand at 682.606 kb on - strand at 682.606 kb on - strand at 682.606 kb on - strand at 682.636 kb on + strand at 682.636 kb on + strand at 682.636 kb on + strand at 682.636 kb on + strand at 682.637 kb on - strand at 682.637 kb on - strand at 682.637 kb on - strand at 682.673 kb on - strand at 682.791 kb on + strand, within PFLU_RS02965 at 683.156 kb on + strand, within PFLU_RS02965 at 683.157 kb on - strand, within PFLU_RS02965 at 683.157 kb on - strand, within PFLU_RS02965 at 683.184 kb on + strand, within PFLU_RS02965 at 683.184 kb on + strand, within PFLU_RS02965 at 683.185 kb on - strand, within PFLU_RS02965 at 683.231 kb on + strand, within PFLU_RS02965 at 683.292 kb on + strand, within PFLU_RS02965 at 683.293 kb on - strand, within PFLU_RS02965 at 683.294 kb on + strand, within PFLU_RS02965 at 683.294 kb on + strand, within PFLU_RS02965 at 683.294 kb on + strand, within PFLU_RS02965 at 683.294 kb on + strand, within PFLU_RS02965 at 683.294 kb on + strand, within PFLU_RS02965 at 683.294 kb on + strand, within PFLU_RS02965 at 683.294 kb on + strand, within PFLU_RS02965 at 683.294 kb on + strand, within PFLU_RS02965 at 683.294 kb on + strand, within PFLU_RS02965 at 683.294 kb on + strand, within PFLU_RS02965 at 683.294 kb on + strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.298 kb on + strand, within PFLU_RS02965 at 683.298 kb on + strand, within PFLU_RS02965 at 683.298 kb on + strand, within PFLU_RS02965 at 683.298 kb on + strand, within PFLU_RS02965 at 683.298 kb on + strand, within PFLU_RS02965 at 683.298 kb on + strand, within PFLU_RS02965 at 683.298 kb on + strand, within PFLU_RS02965 at 683.298 kb on + strand, within PFLU_RS02965 at 683.298 kb on + strand, within PFLU_RS02965 at 683.298 kb on + strand, within PFLU_RS02965 at 683.298 kb on + strand, within PFLU_RS02965 at 683.298 kb on + strand, within PFLU_RS02965 at 683.299 kb on - strand at 683.299 kb on - strand at 683.299 kb on - strand at 683.299 kb on - strand at 683.299 kb on - strand at 683.299 kb on - strand at 683.299 kb on - strand at 683.299 kb on - strand at 683.299 kb on - strand at 683.299 kb on - strand at 683.299 kb on - strand at 683.299 kb on - strand at 683.299 kb on - strand at 683.299 kb on - strand at 683.299 kb on - strand at 683.299 kb on - strand at 683.316 kb on + strand at 683.316 kb on + strand at 683.316 kb on + strand at 683.316 kb on + strand at 683.316 kb on + strand at 683.316 kb on + strand at 683.317 kb on - strand at 683.371 kb on - strand at 683.371 kb on - strand at 683.420 kb on + strand at 683.420 kb on + strand at 683.421 kb on - strand at 683.556 kb on + strand, within PFLU_RS02970 at 683.556 kb on + strand, within PFLU_RS02970 at 683.557 kb on - strand, within PFLU_RS02970 at 683.573 kb on + strand, within PFLU_RS02970 at 683.897 kb on + strand, within PFLU_RS02970 at 683.942 kb on + strand, within PFLU_RS02970 at 684.075 kb on - strand, within PFLU_RS02970 at 684.331 kb on + strand, within PFLU_RS02970 at 684.331 kb on + strand, within PFLU_RS02970 at 684.437 kb on + strand, within PFLU_RS02970 at 684.437 kb on + strand, within PFLU_RS02970 at 684.438 kb on - strand, within PFLU_RS02970 at 684.438 kb on - strand, within PFLU_RS02970 at 684.438 kb on - strand, within PFLU_RS02970 at 684.474 kb on + strand at 684.474 kb on + strand at 684.474 kb on + strand at 684.474 kb on + strand at 684.475 kb on - strand at 684.475 kb on - strand at 684.476 kb on + strand at 684.476 kb on + strand at 684.476 kb on + strand at 684.477 kb on - strand at 684.550 kb on - strand at 684.550 kb on - strand at 684.572 kb on + strand at 684.572 kb on + strand at 684.572 kb on + strand at 684.572 kb on + strand at 684.572 kb on + strand at 684.573 kb on - strand at 684.573 kb on - strand at 684.633 kb on + strand at 684.634 kb on - strand at 684.634 kb on - strand at 684.634 kb on - strand at 684.634 kb on - strand at 684.634 kb on - strand at 684.634 kb on - strand at 684.636 kb on + strand at 684.647 kb on + strand at 684.691 kb on + strand at 684.691 kb on + strand at 684.691 kb on + strand at 684.691 kb on + strand at 684.692 kb on - strand at 684.692 kb on - strand at 684.827 kb on + strand, within PFLU_RS02975 at 684.827 kb on + strand, within PFLU_RS02975 at 684.828 kb on - strand, within PFLU_RS02975 at 684.960 kb on + strand, within PFLU_RS02975 at 684.960 kb on + strand, within PFLU_RS02975 at 684.960 kb on + strand, within PFLU_RS02975 at 684.960 kb on + strand, within PFLU_RS02975 at 684.960 kb on + strand, within PFLU_RS02975 at 684.960 kb on + strand, within PFLU_RS02975 at 684.960 kb on + strand, within PFLU_RS02975 at 684.960 kb on + strand, within PFLU_RS02975 at 684.961 kb on - strand, within PFLU_RS02975 at 684.961 kb on - strand, within PFLU_RS02975 at 684.961 kb on - strand, within PFLU_RS02975 at 684.961 kb on - strand, within PFLU_RS02975 at 684.961 kb on - strand, within PFLU_RS02975 at 684.961 kb on - strand, within PFLU_RS02975 at 684.961 kb on - strand, within PFLU_RS02975 at 685.366 kb on - strand, within PFLU_RS02975 at 685.366 kb on - strand, within PFLU_RS02975 at 685.425 kb on - strand, within PFLU_RS02975 at 685.451 kb on + strand, within PFLU_RS02975 at 685.451 kb on + strand, within PFLU_RS02975 at 685.452 kb on - strand, within PFLU_RS02975 at 685.452 kb on - strand, within PFLU_RS02975
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C); with MOPS remove 682,544 - +0.5 682,571 + -1.1 682,571 + +0.6 682,586 + -0.4 682,586 + -2.0 682,586 + -0.8 682,586 + -0.1 682,586 + +0.6 682,586 + +0.4 682,587 - -1.5 682,587 - -0.6 682,587 - +0.8 682,587 - +0.3 682,587 - -0.7 682,587 - -0.2 682,587 - -0.1 682,587 - -0.3 682,588 + +0.0 682,588 + +1.3 682,588 + +0.2 682,588 + +0.2 682,588 + -1.4 682,588 + +1.7 682,588 + -0.8 682,588 + -0.7 682,588 + +0.7 682,588 + +0.2 682,588 + -0.9 682,588 + -0.6 682,588 + +0.5 682,588 + +0.3 682,589 - -0.4 682,589 - -0.8 682,589 - -0.3 682,589 - +0.1 682,589 - -0.6 682,589 - +0.6 682,589 - -0.1 682,589 - +0.4 682,589 - -2.1 682,589 - -0.4 682,589 - +0.1 682,605 + +1.6 682,605 + -1.3 682,605 + +0.6 682,606 - +0.9 682,606 - -0.9 682,606 - +1.6 682,636 + -0.7 682,636 + -0.5 682,636 + -1.1 682,636 + +0.4 682,637 - +0.3 682,637 - -0.9 682,637 - +0.6 682,673 - -1.4 682,791 + PFLU_RS02965 0.19 +1.2 683,156 + PFLU_RS02965 0.70 -2.1 683,157 - PFLU_RS02965 0.70 -1.9 683,157 - PFLU_RS02965 0.70 -0.3 683,184 + PFLU_RS02965 0.74 -3.2 683,184 + PFLU_RS02965 0.74 -0.7 683,185 - PFLU_RS02965 0.74 -1.4 683,231 + PFLU_RS02965 0.81 +0.2 683,292 + PFLU_RS02965 0.89 -0.0 683,293 - PFLU_RS02965 0.89 -1.1 683,294 + PFLU_RS02965 0.89 -1.2 683,294 + PFLU_RS02965 0.89 -1.0 683,294 + PFLU_RS02965 0.89 +0.5 683,294 + PFLU_RS02965 0.89 -0.4 683,294 + PFLU_RS02965 0.89 -0.3 683,294 + PFLU_RS02965 0.89 +0.2 683,294 + PFLU_RS02965 0.89 -0.3 683,294 + PFLU_RS02965 0.89 -1.4 683,294 + PFLU_RS02965 0.89 -0.3 683,294 + PFLU_RS02965 0.89 +0.7 683,294 + PFLU_RS02965 0.89 -1.0 683,295 - PFLU_RS02965 0.89 -1.8 683,295 - PFLU_RS02965 0.89 -2.2 683,295 - PFLU_RS02965 0.89 -1.4 683,295 - PFLU_RS02965 0.89 -0.6 683,295 - PFLU_RS02965 0.89 -1.0 683,295 - PFLU_RS02965 0.89 +0.1 683,295 - PFLU_RS02965 0.89 -1.1 683,295 - PFLU_RS02965 0.89 -0.2 683,295 - PFLU_RS02965 0.89 +0.7 683,295 - PFLU_RS02965 0.89 -0.0 683,295 - PFLU_RS02965 0.89 -0.8 683,295 - PFLU_RS02965 0.89 -2.5 683,295 - PFLU_RS02965 0.89 -0.7 683,295 - PFLU_RS02965 0.89 -1.1 683,295 - PFLU_RS02965 0.89 +0.9 683,295 - PFLU_RS02965 0.89 -1.8 683,298 + PFLU_RS02965 0.90 +0.3 683,298 + PFLU_RS02965 0.90 -1.1 683,298 + PFLU_RS02965 0.90 -0.6 683,298 + PFLU_RS02965 0.90 -1.1 683,298 + PFLU_RS02965 0.90 -0.2 683,298 + PFLU_RS02965 0.90 -0.4 683,298 + PFLU_RS02965 0.90 -0.1 683,298 + PFLU_RS02965 0.90 +0.2 683,298 + PFLU_RS02965 0.90 +1.0 683,298 + PFLU_RS02965 0.90 -0.6 683,298 + PFLU_RS02965 0.90 +0.4 683,298 + PFLU_RS02965 0.90 +1.2 683,299 - -0.6 683,299 - -1.0 683,299 - -2.7 683,299 - -1.5 683,299 - -1.2 683,299 - -1.8 683,299 - +0.6 683,299 - +0.6 683,299 - -0.6 683,299 - +0.3 683,299 - -1.9 683,299 - +0.1 683,299 - -3.5 683,299 - -2.6 683,299 - -1.2 683,299 - -0.7 683,316 + -0.1 683,316 + +1.5 683,316 + -1.0 683,316 + -1.7 683,316 + -1.0 683,316 + -0.7 683,317 - -0.4 683,371 - +0.9 683,371 - -1.6 683,420 + -2.7 683,420 + -2.2 683,421 - -2.3 683,556 + PFLU_RS02970 0.16 -0.8 683,556 + PFLU_RS02970 0.16 -0.4 683,557 - PFLU_RS02970 0.16 -2.5 683,573 + PFLU_RS02970 0.17 -1.4 683,897 + PFLU_RS02970 0.44 -0.7 683,942 + PFLU_RS02970 0.48 -0.4 684,075 - PFLU_RS02970 0.59 -1.7 684,331 + PFLU_RS02970 0.80 -0.7 684,331 + PFLU_RS02970 0.80 +0.7 684,437 + PFLU_RS02970 0.89 -1.2 684,437 + PFLU_RS02970 0.89 -1.1 684,438 - PFLU_RS02970 0.89 +1.1 684,438 - PFLU_RS02970 0.89 -2.4 684,438 - PFLU_RS02970 0.89 -2.5 684,474 + +0.6 684,474 + -2.6 684,474 + -2.9 684,474 + -0.3 684,475 - -1.4 684,475 - -2.1 684,476 + -1.7 684,476 + +0.1 684,476 + -0.9 684,477 - -1.9 684,550 - -1.4 684,550 - -1.2 684,572 + -1.5 684,572 + -0.6 684,572 + +0.3 684,572 + +0.3 684,572 + -2.7 684,573 - +0.7 684,573 - +0.2 684,633 + +0.4 684,634 - -0.0 684,634 - -0.4 684,634 - -1.8 684,634 - -1.0 684,634 - -2.5 684,634 - +0.3 684,636 + -1.0 684,647 + -0.4 684,691 + +1.0 684,691 + -0.9 684,691 + -1.6 684,691 + -1.7 684,692 - -3.3 684,692 - -0.3 684,827 + PFLU_RS02975 0.16 -1.8 684,827 + PFLU_RS02975 0.16 -0.3 684,828 - PFLU_RS02975 0.16 -2.8 684,960 + PFLU_RS02975 0.26 -0.3 684,960 + PFLU_RS02975 0.26 -2.3 684,960 + PFLU_RS02975 0.26 +0.3 684,960 + PFLU_RS02975 0.26 -3.1 684,960 + PFLU_RS02975 0.26 +0.7 684,960 + PFLU_RS02975 0.26 +0.3 684,960 + PFLU_RS02975 0.26 -0.8 684,960 + PFLU_RS02975 0.26 -2.0 684,961 - PFLU_RS02975 0.26 -0.1 684,961 - PFLU_RS02975 0.26 -1.0 684,961 - PFLU_RS02975 0.26 -0.4 684,961 - PFLU_RS02975 0.26 -1.8 684,961 - PFLU_RS02975 0.26 -1.5 684,961 - PFLU_RS02975 0.26 -1.5 684,961 - PFLU_RS02975 0.26 +0.1 685,366 - PFLU_RS02975 0.56 -0.3 685,366 - PFLU_RS02975 0.56 -0.2 685,425 - PFLU_RS02975 0.60 -1.4 685,451 + PFLU_RS02975 0.62 -1.1 685,451 + PFLU_RS02975 0.62 -1.8 685,452 - PFLU_RS02975 0.62 -2.5 685,452 - PFLU_RS02975 0.62 -1.3
Or see this region's nucleotide sequence