Experiment: Trisodium citrate (C); with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS02170 and PFLU_RS02175 are separated by 396 nucleotides PFLU_RS02175 and PFLU_RS02180 are separated by 47 nucleotides PFLU_RS02180 and PFLU_RS02185 are separated by 236 nucleotides
PFLU_RS02170: PFLU_RS02170 - ParA family protein, at 486,322 to 487,092
_RS02170
PFLU_RS02175: PFLU_RS02175 - hypothetical protein, at 487,489 to 487,710
_RS02175
PFLU_RS02180: PFLU_RS02180 - thioredoxin TrxC, at 487,758 to 488,192
_RS02180
PFLU_RS02185: PFLU_RS02185 - type II toxin-antitoxin system HigB family toxin, at 488,429 to 488,725
_RS02185
Position (kb) 487
488 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 486.559 kb on - strand, within PFLU_RS02170 at 486.562 kb on + strand, within PFLU_RS02170 at 486.562 kb on + strand, within PFLU_RS02170 at 486.562 kb on + strand, within PFLU_RS02170 at 486.562 kb on + strand, within PFLU_RS02170 at 486.562 kb on + strand, within PFLU_RS02170 at 486.562 kb on + strand, within PFLU_RS02170 at 486.563 kb on - strand, within PFLU_RS02170 at 486.645 kb on + strand, within PFLU_RS02170 at 486.645 kb on + strand, within PFLU_RS02170 at 486.645 kb on + strand, within PFLU_RS02170 at 486.645 kb on + strand, within PFLU_RS02170 at 486.645 kb on + strand, within PFLU_RS02170 at 486.645 kb on + strand, within PFLU_RS02170 at 486.646 kb on - strand, within PFLU_RS02170 at 486.646 kb on - strand, within PFLU_RS02170 at 486.646 kb on - strand, within PFLU_RS02170 at 486.646 kb on - strand, within PFLU_RS02170 at 486.646 kb on - strand, within PFLU_RS02170 at 486.646 kb on - strand, within PFLU_RS02170 at 486.812 kb on - strand, within PFLU_RS02170 at 486.812 kb on - strand, within PFLU_RS02170 at 486.812 kb on - strand, within PFLU_RS02170 at 486.812 kb on - strand, within PFLU_RS02170 at 486.967 kb on + strand, within PFLU_RS02170 at 486.968 kb on - strand, within PFLU_RS02170 at 487.011 kb on + strand, within PFLU_RS02170 at 487.011 kb on + strand, within PFLU_RS02170 at 487.012 kb on - strand, within PFLU_RS02170 at 487.012 kb on - strand, within PFLU_RS02170 at 487.041 kb on + strand at 487.041 kb on + strand at 487.041 kb on + strand at 487.041 kb on + strand at 487.041 kb on + strand at 487.042 kb on - strand at 487.042 kb on - strand at 487.042 kb on - strand at 487.042 kb on - strand at 487.072 kb on - strand at 487.095 kb on + strand at 487.095 kb on + strand at 487.095 kb on + strand at 487.096 kb on - strand at 487.096 kb on - strand at 487.180 kb on + strand at 487.213 kb on + strand at 487.213 kb on + strand at 487.213 kb on + strand at 487.213 kb on + strand at 487.213 kb on + strand at 487.213 kb on + strand at 487.214 kb on - strand at 487.214 kb on - strand at 487.214 kb on - strand at 487.224 kb on - strand at 487.229 kb on + strand at 487.229 kb on + strand at 487.229 kb on + strand at 487.230 kb on - strand at 487.230 kb on - strand at 487.230 kb on - strand at 487.230 kb on - strand at 487.271 kb on + strand at 487.271 kb on + strand at 487.271 kb on + strand at 487.272 kb on - strand at 487.310 kb on - strand at 487.310 kb on - strand at 487.310 kb on - strand at 487.312 kb on + strand at 487.313 kb on - strand at 487.334 kb on + strand at 487.334 kb on + strand at 487.334 kb on + strand at 487.334 kb on + strand at 487.335 kb on - strand at 487.335 kb on - strand at 487.376 kb on - strand at 487.376 kb on - strand at 487.418 kb on + strand at 487.418 kb on + strand at 487.419 kb on - strand at 487.421 kb on + strand at 487.421 kb on + strand at 487.422 kb on - strand at 487.422 kb on - strand at 487.422 kb on - strand at 487.430 kb on + strand at 487.430 kb on + strand at 487.431 kb on - strand at 487.431 kb on - strand at 487.431 kb on - strand at 487.431 kb on - strand at 487.450 kb on + strand at 487.450 kb on + strand at 487.451 kb on - strand at 487.501 kb on + strand at 487.501 kb on + strand at 487.501 kb on + strand at 487.502 kb on - strand at 487.502 kb on - strand at 487.502 kb on - strand at 487.503 kb on + strand at 487.503 kb on + strand at 487.503 kb on + strand at 487.503 kb on + strand at 487.503 kb on + strand at 487.504 kb on - strand at 487.504 kb on - strand at 487.504 kb on - strand at 487.504 kb on - strand at 487.504 kb on - strand at 487.549 kb on + strand, within PFLU_RS02175 at 487.549 kb on + strand, within PFLU_RS02175 at 487.549 kb on + strand, within PFLU_RS02175 at 487.549 kb on + strand, within PFLU_RS02175 at 487.549 kb on + strand, within PFLU_RS02175 at 487.550 kb on - strand, within PFLU_RS02175 at 487.550 kb on - strand, within PFLU_RS02175 at 487.550 kb on - strand, within PFLU_RS02175 at 487.550 kb on - strand, within PFLU_RS02175 at 487.658 kb on + strand, within PFLU_RS02175 at 487.658 kb on + strand, within PFLU_RS02175 at 487.659 kb on - strand, within PFLU_RS02175 at 487.659 kb on - strand, within PFLU_RS02175 at 487.708 kb on + strand at 487.709 kb on - strand at 487.709 kb on - strand at 487.759 kb on + strand at 487.759 kb on + strand at 487.980 kb on - strand, within PFLU_RS02180 at 488.175 kb on + strand at 488.175 kb on + strand at 488.175 kb on + strand at 488.176 kb on - strand at 488.176 kb on - strand at 488.224 kb on + strand at 488.224 kb on + strand at 488.224 kb on + strand at 488.224 kb on + strand at 488.224 kb on + strand at 488.224 kb on + strand at 488.225 kb on - strand at 488.225 kb on - strand at 488.225 kb on - strand at 488.225 kb on - strand at 488.225 kb on - strand at 488.247 kb on + strand at 488.248 kb on - strand at 488.248 kb on - strand at 488.248 kb on - strand at 488.271 kb on + strand at 488.272 kb on - strand at 488.272 kb on - strand at 488.272 kb on - strand at 488.311 kb on + strand at 488.315 kb on + strand at 488.315 kb on + strand at 488.315 kb on + strand at 488.315 kb on + strand at 488.315 kb on + strand at 488.315 kb on + strand at 488.315 kb on + strand at 488.315 kb on + strand at 488.315 kb on + strand at 488.315 kb on + strand at 488.315 kb on + strand at 488.315 kb on + strand at 488.315 kb on + strand at 488.315 kb on + strand at 488.315 kb on + strand at 488.315 kb on + strand at 488.315 kb on + strand at 488.315 kb on + strand at 488.316 kb on - strand at 488.316 kb on - strand at 488.316 kb on - strand at 488.316 kb on - strand at 488.316 kb on - strand at 488.316 kb on - strand at 488.316 kb on - strand at 488.316 kb on - strand at 488.316 kb on - strand at 488.316 kb on - strand at 488.395 kb on + strand at 488.404 kb on + strand at 488.404 kb on + strand at 488.514 kb on + strand, within PFLU_RS02185 at 488.514 kb on + strand, within PFLU_RS02185 at 488.515 kb on - strand, within PFLU_RS02185 at 488.614 kb on + strand, within PFLU_RS02185 at 488.614 kb on + strand, within PFLU_RS02185 at 488.615 kb on - strand, within PFLU_RS02185 at 488.660 kb on + strand, within PFLU_RS02185 at 488.660 kb on + strand, within PFLU_RS02185 at 488.660 kb on + strand, within PFLU_RS02185 at 488.661 kb on - strand, within PFLU_RS02185 at 488.661 kb on - strand, within PFLU_RS02185 at 488.690 kb on + strand, within PFLU_RS02185 at 488.691 kb on - strand, within PFLU_RS02185 at 488.701 kb on + strand at 488.701 kb on + strand at 488.701 kb on + strand at 488.701 kb on + strand at 488.701 kb on + strand at 488.702 kb on - strand at 488.702 kb on - strand at 488.702 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C); with MOPS remove 486,559 - PFLU_RS02170 0.31 -0.5 486,562 + PFLU_RS02170 0.31 -0.4 486,562 + PFLU_RS02170 0.31 +0.3 486,562 + PFLU_RS02170 0.31 +0.1 486,562 + PFLU_RS02170 0.31 -1.0 486,562 + PFLU_RS02170 0.31 -0.3 486,562 + PFLU_RS02170 0.31 +0.3 486,563 - PFLU_RS02170 0.31 -1.6 486,645 + PFLU_RS02170 0.42 +0.5 486,645 + PFLU_RS02170 0.42 +1.4 486,645 + PFLU_RS02170 0.42 -0.9 486,645 + PFLU_RS02170 0.42 -0.3 486,645 + PFLU_RS02170 0.42 +0.8 486,645 + PFLU_RS02170 0.42 +0.1 486,646 - PFLU_RS02170 0.42 -0.2 486,646 - PFLU_RS02170 0.42 -0.3 486,646 - PFLU_RS02170 0.42 -0.7 486,646 - PFLU_RS02170 0.42 -0.3 486,646 - PFLU_RS02170 0.42 +0.3 486,646 - PFLU_RS02170 0.42 -0.2 486,812 - PFLU_RS02170 0.64 -0.6 486,812 - PFLU_RS02170 0.64 -0.2 486,812 - PFLU_RS02170 0.64 -1.8 486,812 - PFLU_RS02170 0.64 -0.9 486,967 + PFLU_RS02170 0.84 -0.9 486,968 - PFLU_RS02170 0.84 +1.8 487,011 + PFLU_RS02170 0.89 -1.4 487,011 + PFLU_RS02170 0.89 -0.2 487,012 - PFLU_RS02170 0.89 -0.1 487,012 - PFLU_RS02170 0.89 +0.2 487,041 + +1.3 487,041 + +0.8 487,041 + +0.7 487,041 + +0.7 487,041 + +0.6 487,042 - -0.9 487,042 - +0.2 487,042 - +0.1 487,042 - -0.7 487,072 - +1.0 487,095 + +0.3 487,095 + +0.7 487,095 + -0.7 487,096 - -0.9 487,096 - -0.0 487,180 + +0.3 487,213 + +0.4 487,213 + -0.5 487,213 + +0.1 487,213 + +0.0 487,213 + -0.8 487,213 + +0.0 487,214 - +0.6 487,214 - -1.5 487,214 - +1.1 487,224 - +0.6 487,229 + -0.3 487,229 + -0.1 487,229 + +0.6 487,230 - +0.4 487,230 - +0.7 487,230 - +1.0 487,230 - -0.0 487,271 + -0.6 487,271 + +2.5 487,271 + -2.0 487,272 - -0.3 487,310 - -0.3 487,310 - +0.8 487,310 - +0.3 487,312 + +0.5 487,313 - -0.4 487,334 + +0.5 487,334 + +1.1 487,334 + +0.6 487,334 + +1.1 487,335 - +0.7 487,335 - +0.6 487,376 - +0.2 487,376 - +0.2 487,418 + -0.0 487,418 + +0.1 487,419 - -1.6 487,421 + +0.3 487,421 + +0.7 487,422 - -0.4 487,422 - +0.5 487,422 - -0.4 487,430 + -0.2 487,430 + +0.2 487,431 - +0.2 487,431 - +0.2 487,431 - -1.5 487,431 - +0.5 487,450 + +1.3 487,450 + +1.2 487,451 - -0.1 487,501 + -0.6 487,501 + +0.7 487,501 + -0.2 487,502 - -2.6 487,502 - -0.7 487,502 - +1.0 487,503 + +0.6 487,503 + +0.6 487,503 + +0.4 487,503 + +0.1 487,503 + -0.2 487,504 - -0.5 487,504 - +0.5 487,504 - +1.9 487,504 - -1.2 487,504 - +0.9 487,549 + PFLU_RS02175 0.27 -0.6 487,549 + PFLU_RS02175 0.27 +1.0 487,549 + PFLU_RS02175 0.27 -1.3 487,549 + PFLU_RS02175 0.27 -0.9 487,549 + PFLU_RS02175 0.27 -0.7 487,550 - PFLU_RS02175 0.27 -1.7 487,550 - PFLU_RS02175 0.27 -0.3 487,550 - PFLU_RS02175 0.27 +0.4 487,550 - PFLU_RS02175 0.27 +1.1 487,658 + PFLU_RS02175 0.76 -0.7 487,658 + PFLU_RS02175 0.76 -0.1 487,659 - PFLU_RS02175 0.77 -1.0 487,659 - PFLU_RS02175 0.77 +0.5 487,708 + +0.9 487,709 - +1.3 487,709 - +0.7 487,759 + +1.0 487,759 + -0.4 487,980 - PFLU_RS02180 0.51 -1.0 488,175 + -0.2 488,175 + -0.4 488,175 + -0.4 488,176 - -1.2 488,176 - +1.3 488,224 + +0.9 488,224 + -1.4 488,224 + +1.9 488,224 + +3.1 488,224 + +0.8 488,224 + +1.0 488,225 - +0.3 488,225 - -0.2 488,225 - -1.1 488,225 - +1.0 488,225 - -1.2 488,247 + -0.4 488,248 - +0.6 488,248 - -0.1 488,248 - -1.4 488,271 + -0.5 488,272 - +0.3 488,272 - +0.9 488,272 - +0.7 488,311 + -0.2 488,315 + -0.3 488,315 + +0.8 488,315 + +0.5 488,315 + -1.1 488,315 + -0.6 488,315 + +1.1 488,315 + +0.4 488,315 + -1.5 488,315 + +0.7 488,315 + -0.4 488,315 + +0.2 488,315 + +1.3 488,315 + +0.3 488,315 + -1.9 488,315 + -0.7 488,315 + -0.1 488,315 + +1.0 488,315 + +0.9 488,316 - +0.1 488,316 - -1.3 488,316 - -0.5 488,316 - -0.5 488,316 - +1.1 488,316 - +0.1 488,316 - -0.8 488,316 - +1.6 488,316 - +1.7 488,316 - -0.5 488,395 + +0.7 488,404 + +0.2 488,404 + +0.1 488,514 + PFLU_RS02185 0.29 -0.0 488,514 + PFLU_RS02185 0.29 +0.4 488,515 - PFLU_RS02185 0.29 -0.3 488,614 + PFLU_RS02185 0.62 +0.3 488,614 + PFLU_RS02185 0.62 +0.0 488,615 - PFLU_RS02185 0.63 +0.6 488,660 + PFLU_RS02185 0.78 -2.1 488,660 + PFLU_RS02185 0.78 +0.2 488,660 + PFLU_RS02185 0.78 +0.4 488,661 - PFLU_RS02185 0.78 +1.2 488,661 - PFLU_RS02185 0.78 +0.9 488,690 + PFLU_RS02185 0.88 +0.1 488,691 - PFLU_RS02185 0.88 +1.1 488,701 + +0.4 488,701 + +0.2 488,701 + -0.1 488,701 + -0.4 488,701 + +1.0 488,702 - -1.3 488,702 - +0.4 488,702 - -0.0
Or see this region's nucleotide sequence