Experiment: Trisodium citrate (C); with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS01100 and PFLU_RS30340 overlap by 20 nucleotides PFLU_RS30340 and PFLU_RS01110 are separated by 318 nucleotides PFLU_RS01110 and PFLU_RS31375 are separated by 820 nucleotides
PFLU_RS01100: PFLU_RS01100 - recombinase family protein, at 243,384 to 243,950
_RS01100
PFLU_RS30340: PFLU_RS30340 - hypothetical protein, at 243,931 to 244,169
_RS30340
PFLU_RS01110: PFLU_RS01110 - AAA family ATPase, at 244,488 to 245,112
_RS01110
PFLU_RS31375: PFLU_RS31375 - hypothetical protein, at 245,933 to 246,113
_RS31375
Position (kb) 244
245
246 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 243.511 kb on + strand, within PFLU_RS01100 at 243.539 kb on - strand, within PFLU_RS01100 at 243.551 kb on + strand, within PFLU_RS01100 at 243.551 kb on + strand, within PFLU_RS01100 at 243.551 kb on + strand, within PFLU_RS01100 at 243.551 kb on + strand, within PFLU_RS01100 at 243.551 kb on + strand, within PFLU_RS01100 at 243.551 kb on + strand, within PFLU_RS01100 at 243.551 kb on + strand, within PFLU_RS01100 at 243.551 kb on + strand, within PFLU_RS01100 at 243.551 kb on + strand, within PFLU_RS01100 at 243.551 kb on + strand, within PFLU_RS01100 at 243.552 kb on - strand, within PFLU_RS01100 at 243.552 kb on - strand, within PFLU_RS01100 at 243.552 kb on - strand, within PFLU_RS01100 at 243.552 kb on - strand, within PFLU_RS01100 at 243.552 kb on - strand, within PFLU_RS01100 at 243.552 kb on - strand, within PFLU_RS01100 at 243.552 kb on - strand, within PFLU_RS01100 at 243.556 kb on + strand, within PFLU_RS01100 at 243.557 kb on - strand, within PFLU_RS01100 at 243.557 kb on - strand, within PFLU_RS01100 at 243.557 kb on - strand, within PFLU_RS01100 at 243.557 kb on - strand, within PFLU_RS01100 at 243.557 kb on - strand, within PFLU_RS01100 at 243.557 kb on - strand, within PFLU_RS01100 at 243.573 kb on + strand, within PFLU_RS01100 at 243.574 kb on - strand, within PFLU_RS01100 at 243.686 kb on - strand, within PFLU_RS01100 at 243.686 kb on - strand, within PFLU_RS01100 at 243.780 kb on + strand, within PFLU_RS01100 at 243.780 kb on + strand, within PFLU_RS01100 at 243.792 kb on + strand, within PFLU_RS01100 at 243.837 kb on + strand, within PFLU_RS01100 at 243.903 kb on + strand at 243.903 kb on + strand at 243.903 kb on + strand at 243.903 kb on + strand at 243.903 kb on + strand at 243.903 kb on + strand at 243.904 kb on - strand at 243.904 kb on - strand at 243.904 kb on - strand at 243.904 kb on - strand at 243.904 kb on - strand at 243.904 kb on - strand at 243.909 kb on + strand at 243.909 kb on + strand at 243.909 kb on + strand at 243.909 kb on + strand at 243.909 kb on + strand at 243.909 kb on + strand at 243.909 kb on + strand at 243.909 kb on + strand at 243.909 kb on + strand at 243.909 kb on + strand at 243.909 kb on + strand at 243.910 kb on - strand at 243.910 kb on - strand at 243.910 kb on - strand at 243.910 kb on - strand at 243.910 kb on - strand at 243.910 kb on - strand at 243.910 kb on - strand at 243.910 kb on - strand at 243.910 kb on - strand at 243.910 kb on - strand at 243.910 kb on - strand at 243.910 kb on - strand at 243.910 kb on - strand at 243.910 kb on - strand at 243.910 kb on - strand at 243.910 kb on - strand at 243.949 kb on - strand at 244.094 kb on + strand, within PFLU_RS30340 at 244.100 kb on + strand, within PFLU_RS30340 at 244.101 kb on - strand, within PFLU_RS30340 at 244.101 kb on - strand, within PFLU_RS30340 at 244.101 kb on - strand, within PFLU_RS30340 at 244.172 kb on + strand at 244.172 kb on + strand at 244.172 kb on + strand at 244.172 kb on + strand at 244.172 kb on + strand at 244.172 kb on + strand at 244.172 kb on + strand at 244.172 kb on + strand at 244.173 kb on - strand at 244.173 kb on - strand at 244.173 kb on - strand at 244.173 kb on - strand at 244.173 kb on - strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.253 kb on - strand at 244.253 kb on - strand at 244.253 kb on - strand at 244.253 kb on - strand at 244.253 kb on - strand at 244.253 kb on - strand at 244.253 kb on - strand at 244.253 kb on - strand at 244.253 kb on - strand at 244.253 kb on - strand at 244.279 kb on + strand at 244.279 kb on + strand at 244.279 kb on + strand at 244.279 kb on + strand at 244.279 kb on + strand at 244.279 kb on + strand at 244.279 kb on + strand at 244.280 kb on - strand at 244.280 kb on - strand at 244.280 kb on - strand at 244.280 kb on - strand at 244.280 kb on - strand at 244.280 kb on - strand at 244.280 kb on - strand at 244.349 kb on + strand at 244.349 kb on + strand at 244.349 kb on + strand at 244.349 kb on + strand at 244.350 kb on - strand at 244.390 kb on + strand at 244.405 kb on + strand at 244.405 kb on + strand at 244.405 kb on + strand at 244.405 kb on + strand at 244.405 kb on + strand at 244.405 kb on + strand at 244.405 kb on + strand at 244.405 kb on + strand at 244.405 kb on + strand at 244.405 kb on + strand at 244.405 kb on + strand at 244.405 kb on + strand at 244.405 kb on + strand at 244.405 kb on + strand at 244.406 kb on - strand at 244.406 kb on - strand at 244.406 kb on - strand at 244.406 kb on - strand at 244.406 kb on - strand at 244.406 kb on - strand at 244.406 kb on - strand at 244.406 kb on - strand at 244.406 kb on - strand at 244.406 kb on - strand at 244.406 kb on - strand at 244.406 kb on - strand at 244.406 kb on - strand at 244.406 kb on - strand at 244.406 kb on - strand at 244.493 kb on + strand at 244.493 kb on + strand at 244.493 kb on + strand at 244.494 kb on - strand at 244.494 kb on - strand at 244.494 kb on - strand at 244.643 kb on - strand, within PFLU_RS01110 at 244.830 kb on + strand, within PFLU_RS01110 at 244.830 kb on + strand, within PFLU_RS01110 at 244.831 kb on - strand, within PFLU_RS01110 at 244.831 kb on - strand, within PFLU_RS01110 at 244.835 kb on + strand, within PFLU_RS01110 at 244.835 kb on + strand, within PFLU_RS01110 at 244.835 kb on + strand, within PFLU_RS01110 at 244.835 kb on + strand, within PFLU_RS01110 at 245.008 kb on + strand, within PFLU_RS01110 at 245.008 kb on + strand, within PFLU_RS01110 at 245.008 kb on + strand, within PFLU_RS01110 at 245.008 kb on + strand, within PFLU_RS01110 at 245.008 kb on + strand, within PFLU_RS01110 at 245.008 kb on + strand, within PFLU_RS01110 at 245.009 kb on - strand, within PFLU_RS01110 at 245.009 kb on - strand, within PFLU_RS01110 at 245.009 kb on - strand, within PFLU_RS01110 at 245.087 kb on + strand at 245.087 kb on + strand at 245.087 kb on + strand at 245.087 kb on + strand at 245.088 kb on - strand at 245.088 kb on - strand at 245.265 kb on + strand at 245.266 kb on - strand at 245.266 kb on - strand at 245.266 kb on - strand at 245.266 kb on - strand at 245.329 kb on + strand at 245.329 kb on + strand at 245.329 kb on + strand at 245.329 kb on + strand at 245.329 kb on + strand at 245.329 kb on + strand at 245.329 kb on + strand at 245.329 kb on + strand at 245.330 kb on - strand at 245.330 kb on - strand at 245.330 kb on - strand at 245.330 kb on - strand at 245.330 kb on - strand at 245.330 kb on - strand at 245.330 kb on - strand at 245.333 kb on + strand at 245.333 kb on + strand at 245.334 kb on - strand at 245.334 kb on - strand at 245.334 kb on - strand at 245.353 kb on + strand at 245.361 kb on - strand at 245.361 kb on - strand at 245.368 kb on - strand at 245.395 kb on + strand at 245.407 kb on - strand at 245.480 kb on + strand at 245.492 kb on - strand at 245.539 kb on - strand at 245.542 kb on + strand at 245.543 kb on - strand at 245.543 kb on - strand at 245.543 kb on - strand at 245.593 kb on + strand at 245.594 kb on - strand at 245.618 kb on - strand at 245.623 kb on + strand at 245.623 kb on + strand at 245.624 kb on - strand at 245.624 kb on - strand at 245.626 kb on + strand at 245.626 kb on + strand at 245.626 kb on + strand at 245.626 kb on + strand at 245.626 kb on + strand at 245.626 kb on + strand at 245.626 kb on + strand at 245.626 kb on + strand at 245.626 kb on + strand at 245.626 kb on + strand at 245.626 kb on + strand at 245.627 kb on - strand at 245.627 kb on - strand at 245.627 kb on - strand at 245.627 kb on - strand at 245.627 kb on - strand at 245.627 kb on - strand at 245.627 kb on - strand at 245.627 kb on - strand at 245.627 kb on - strand at 245.627 kb on - strand at 245.627 kb on - strand at 245.627 kb on - strand at 245.627 kb on - strand at 245.627 kb on - strand at 245.627 kb on - strand at 245.627 kb on - strand at 245.627 kb on - strand at 245.627 kb on - strand at 245.634 kb on + strand at 245.634 kb on + strand at 245.702 kb on + strand at 245.726 kb on - strand at 245.728 kb on + strand at 245.740 kb on + strand at 245.791 kb on + strand at 245.834 kb on + strand at 245.849 kb on - strand at 245.855 kb on + strand at 245.964 kb on + strand, within PFLU_RS31375 at 245.979 kb on + strand, within PFLU_RS31375 at 245.980 kb on - strand, within PFLU_RS31375 at 245.980 kb on - strand, within PFLU_RS31375 at 245.980 kb on - strand, within PFLU_RS31375 at 245.980 kb on - strand, within PFLU_RS31375 at 246.045 kb on + strand, within PFLU_RS31375 at 246.045 kb on + strand, within PFLU_RS31375 at 246.045 kb on + strand, within PFLU_RS31375 at 246.045 kb on + strand, within PFLU_RS31375 at 246.045 kb on + strand, within PFLU_RS31375 at 246.045 kb on + strand, within PFLU_RS31375 at 246.045 kb on + strand, within PFLU_RS31375 at 246.045 kb on + strand, within PFLU_RS31375 at 246.046 kb on - strand, within PFLU_RS31375 at 246.046 kb on - strand, within PFLU_RS31375 at 246.046 kb on - strand, within PFLU_RS31375 at 246.046 kb on - strand, within PFLU_RS31375 at 246.046 kb on - strand, within PFLU_RS31375 at 246.046 kb on - strand, within PFLU_RS31375 at 246.046 kb on - strand, within PFLU_RS31375 at 246.048 kb on - strand, within PFLU_RS31375 at 246.098 kb on - strand at 246.098 kb on - strand at 246.098 kb on - strand at 246.111 kb on + strand at 246.111 kb on + strand at 246.111 kb on + strand at 246.111 kb on + strand at 246.111 kb on + strand at 246.112 kb on - strand at 246.112 kb on - strand at 246.112 kb on - strand at 246.112 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C); with MOPS remove 243,511 + PFLU_RS01100 0.22 +1.4 243,539 - PFLU_RS01100 0.27 +0.5 243,551 + PFLU_RS01100 0.29 +1.5 243,551 + PFLU_RS01100 0.29 -0.1 243,551 + PFLU_RS01100 0.29 +0.1 243,551 + PFLU_RS01100 0.29 +0.2 243,551 + PFLU_RS01100 0.29 +1.6 243,551 + PFLU_RS01100 0.29 -1.6 243,551 + PFLU_RS01100 0.29 +0.5 243,551 + PFLU_RS01100 0.29 +0.3 243,551 + PFLU_RS01100 0.29 +0.1 243,551 + PFLU_RS01100 0.29 +0.6 243,552 - PFLU_RS01100 0.30 +0.3 243,552 - PFLU_RS01100 0.30 +0.8 243,552 - PFLU_RS01100 0.30 -0.6 243,552 - PFLU_RS01100 0.30 -0.0 243,552 - PFLU_RS01100 0.30 -0.8 243,552 - PFLU_RS01100 0.30 -0.2 243,552 - PFLU_RS01100 0.30 -2.0 243,556 + PFLU_RS01100 0.30 -0.3 243,557 - PFLU_RS01100 0.31 -1.4 243,557 - PFLU_RS01100 0.31 -0.8 243,557 - PFLU_RS01100 0.31 +0.5 243,557 - PFLU_RS01100 0.31 +0.5 243,557 - PFLU_RS01100 0.31 -1.1 243,557 - PFLU_RS01100 0.31 +0.8 243,573 + PFLU_RS01100 0.33 +1.4 243,574 - PFLU_RS01100 0.34 +0.3 243,686 - PFLU_RS01100 0.53 +0.2 243,686 - PFLU_RS01100 0.53 +0.1 243,780 + PFLU_RS01100 0.70 +1.0 243,780 + PFLU_RS01100 0.70 -0.0 243,792 + PFLU_RS01100 0.72 -0.7 243,837 + PFLU_RS01100 0.80 +0.3 243,903 + +0.8 243,903 + +0.6 243,903 + +0.2 243,903 + -0.7 243,903 + +1.2 243,903 + -2.3 243,904 - -0.1 243,904 - +0.6 243,904 - -0.3 243,904 - +0.6 243,904 - +1.3 243,904 - +0.4 243,909 + -0.6 243,909 + +1.0 243,909 + +0.1 243,909 + +0.4 243,909 + -1.2 243,909 + -0.1 243,909 + -0.3 243,909 + +0.3 243,909 + -0.2 243,909 + -1.0 243,909 + -0.5 243,910 - +0.6 243,910 - +0.6 243,910 - +0.2 243,910 - +0.8 243,910 - -0.2 243,910 - -1.6 243,910 - -1.7 243,910 - -0.0 243,910 - +0.5 243,910 - -0.0 243,910 - +0.1 243,910 - +1.0 243,910 - -0.9 243,910 - +0.6 243,910 - +1.6 243,910 - +0.8 243,949 - +1.0 244,094 + PFLU_RS30340 0.68 -0.9 244,100 + PFLU_RS30340 0.71 -0.1 244,101 - PFLU_RS30340 0.71 +0.4 244,101 - PFLU_RS30340 0.71 +0.9 244,101 - PFLU_RS30340 0.71 -1.0 244,172 + +0.5 244,172 + +1.0 244,172 + -2.4 244,172 + +0.3 244,172 + -1.1 244,172 + +0.9 244,172 + -0.6 244,172 + -0.4 244,173 - +0.9 244,173 - -0.8 244,173 - +0.4 244,173 - +0.9 244,173 - +1.4 244,252 + +0.0 244,252 + +0.6 244,252 + -1.8 244,252 + -0.5 244,252 + +0.3 244,252 + +1.2 244,252 + +1.6 244,252 + +0.1 244,252 + +0.4 244,252 + +1.0 244,252 + -0.7 244,253 - +0.2 244,253 - -2.0 244,253 - +0.3 244,253 - +0.3 244,253 - +0.3 244,253 - +0.5 244,253 - -0.3 244,253 - +0.3 244,253 - +0.7 244,253 - -3.4 244,279 + +0.2 244,279 + +0.8 244,279 + +0.4 244,279 + +0.1 244,279 + +0.5 244,279 + +0.9 244,279 + -0.7 244,280 - +0.3 244,280 - +1.3 244,280 - +0.7 244,280 - -0.8 244,280 - +1.2 244,280 - +0.5 244,280 - -1.6 244,349 + -2.0 244,349 + -1.9 244,349 + +0.9 244,349 + -1.7 244,350 - +0.5 244,390 + +0.6 244,405 + +0.0 244,405 + -0.5 244,405 + -0.9 244,405 + +0.7 244,405 + +1.7 244,405 + -0.6 244,405 + -1.2 244,405 + +0.8 244,405 + +0.6 244,405 + +0.9 244,405 + +0.2 244,405 + -0.7 244,405 + +0.7 244,405 + -0.3 244,406 - -0.7 244,406 - -1.1 244,406 - +0.7 244,406 - -0.4 244,406 - -1.3 244,406 - -0.9 244,406 - +1.2 244,406 - +1.8 244,406 - +0.8 244,406 - +1.1 244,406 - -0.5 244,406 - +1.2 244,406 - -0.1 244,406 - -0.2 244,406 - -1.3 244,493 + -0.9 244,493 + -2.2 244,493 + -0.5 244,494 - +0.9 244,494 - +0.9 244,494 - +0.3 244,643 - PFLU_RS01110 0.25 +0.0 244,830 + PFLU_RS01110 0.55 +0.4 244,830 + PFLU_RS01110 0.55 +1.7 244,831 - PFLU_RS01110 0.55 +0.6 244,831 - PFLU_RS01110 0.55 -0.4 244,835 + PFLU_RS01110 0.56 +1.0 244,835 + PFLU_RS01110 0.56 +0.9 244,835 + PFLU_RS01110 0.56 +1.9 244,835 + PFLU_RS01110 0.56 -0.2 245,008 + PFLU_RS01110 0.83 +0.8 245,008 + PFLU_RS01110 0.83 -0.4 245,008 + PFLU_RS01110 0.83 -0.3 245,008 + PFLU_RS01110 0.83 -0.4 245,008 + PFLU_RS01110 0.83 +0.9 245,008 + PFLU_RS01110 0.83 -0.4 245,009 - PFLU_RS01110 0.83 +1.1 245,009 - PFLU_RS01110 0.83 -1.5 245,009 - PFLU_RS01110 0.83 +0.9 245,087 + -1.9 245,087 + +0.2 245,087 + +0.0 245,087 + +1.3 245,088 - +0.8 245,088 - +0.6 245,265 + -0.2 245,266 - +0.9 245,266 - -0.3 245,266 - -0.1 245,266 - +0.8 245,329 + -1.5 245,329 + +1.1 245,329 + -1.2 245,329 + +0.5 245,329 + +0.7 245,329 + -2.6 245,329 + +0.4 245,329 + +2.7 245,330 - -0.0 245,330 - -1.1 245,330 - -1.5 245,330 - +0.8 245,330 - +1.2 245,330 - +0.8 245,330 - -1.0 245,333 + -1.0 245,333 + +0.8 245,334 - +0.3 245,334 - +0.9 245,334 - -0.2 245,353 + -1.0 245,361 - +1.1 245,361 - +0.9 245,368 - +2.0 245,395 + -0.4 245,407 - -0.4 245,480 + +0.6 245,492 - -2.4 245,539 - -0.3 245,542 + +0.2 245,543 - +0.2 245,543 - -0.9 245,543 - -2.1 245,593 + +0.7 245,594 - -0.1 245,618 - -1.0 245,623 + +0.0 245,623 + -1.7 245,624 - +0.6 245,624 - -0.3 245,626 + +0.0 245,626 + +0.0 245,626 + +1.1 245,626 + -0.8 245,626 + -1.6 245,626 + -0.7 245,626 + -2.7 245,626 + +0.1 245,626 + +0.2 245,626 + -0.1 245,626 + +0.8 245,627 - +0.9 245,627 - +0.2 245,627 - -0.9 245,627 - -0.6 245,627 - -0.8 245,627 - -0.2 245,627 - -0.6 245,627 - +0.1 245,627 - +0.4 245,627 - +0.5 245,627 - +1.9 245,627 - -0.8 245,627 - -1.1 245,627 - +1.5 245,627 - +0.7 245,627 - +0.8 245,627 - -0.1 245,627 - -0.5 245,634 + +0.8 245,634 + +0.4 245,702 + +0.1 245,726 - +1.4 245,728 + -0.5 245,740 + -0.5 245,791 + +0.2 245,834 + -0.9 245,849 - +0.7 245,855 + +0.4 245,964 + PFLU_RS31375 0.17 +0.7 245,979 + PFLU_RS31375 0.25 -0.2 245,980 - PFLU_RS31375 0.26 -0.6 245,980 - PFLU_RS31375 0.26 +0.6 245,980 - PFLU_RS31375 0.26 -0.9 245,980 - PFLU_RS31375 0.26 +0.5 246,045 + PFLU_RS31375 0.62 -0.3 246,045 + PFLU_RS31375 0.62 +0.6 246,045 + PFLU_RS31375 0.62 +1.1 246,045 + PFLU_RS31375 0.62 -0.3 246,045 + PFLU_RS31375 0.62 -1.7 246,045 + PFLU_RS31375 0.62 +0.8 246,045 + PFLU_RS31375 0.62 +0.0 246,045 + PFLU_RS31375 0.62 -0.3 246,046 - PFLU_RS31375 0.62 +0.5 246,046 - PFLU_RS31375 0.62 +0.5 246,046 - PFLU_RS31375 0.62 -0.7 246,046 - PFLU_RS31375 0.62 +0.2 246,046 - PFLU_RS31375 0.62 +0.6 246,046 - PFLU_RS31375 0.62 -2.3 246,046 - PFLU_RS31375 0.62 +0.7 246,048 - PFLU_RS31375 0.64 +1.1 246,098 - +0.9 246,098 - +1.2 246,098 - +0.4 246,111 + -1.2 246,111 + -1.8 246,111 + -0.4 246,111 + +0.2 246,111 + +0.5 246,112 - +0.2 246,112 - -2.7 246,112 - +0.5 246,112 - -0.2
Or see this region's nucleotide sequence