Experiment: MS_media no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3722 and EX28DRAFT_3723 are separated by 61 nucleotides EX28DRAFT_3723 and EX28DRAFT_3724 are separated by 107 nucleotides EX28DRAFT_3724 and EX28DRAFT_3725 overlap by 4 nucleotides EX28DRAFT_3725 and EX28DRAFT_3726 are separated by 104 nucleotides
EX28DRAFT_3722: EX28DRAFT_3722 - hypoxanthine phosphoribosyltransferase, at 398,993 to 399,529
_3722
EX28DRAFT_3723: EX28DRAFT_3723 - Carbonic anhydrase, at 399,591 to 400,253
_3723
EX28DRAFT_3724: EX28DRAFT_3724 - ABC-type multidrug transport system, ATPase component, at 400,361 to 401,287
_3724
EX28DRAFT_3725: EX28DRAFT_3725 - ABC-type polysaccharide/polyol phosphate export systems, permease component, at 401,284 to 402,054
_3725
EX28DRAFT_3726: EX28DRAFT_3726 - Phosphotransferase system, mannose/fructose-specific component IIA, at 402,159 to 402,599
_3726
Position (kb) 400
401
402 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 399.527 kb on + strand at 399.593 kb on - strand at 399.596 kb on + strand at 399.612 kb on - strand at 399.612 kb on - strand at 399.713 kb on - strand, within EX28DRAFT_3723 at 399.785 kb on + strand, within EX28DRAFT_3723 at 399.787 kb on + strand, within EX28DRAFT_3723 at 399.787 kb on + strand, within EX28DRAFT_3723 at 399.787 kb on + strand, within EX28DRAFT_3723 at 399.787 kb on + strand, within EX28DRAFT_3723 at 399.787 kb on + strand, within EX28DRAFT_3723 at 399.788 kb on - strand, within EX28DRAFT_3723 at 399.788 kb on - strand, within EX28DRAFT_3723 at 399.788 kb on - strand, within EX28DRAFT_3723 at 399.864 kb on - strand, within EX28DRAFT_3723 at 399.864 kb on - strand, within EX28DRAFT_3723 at 399.864 kb on - strand, within EX28DRAFT_3723 at 399.864 kb on - strand, within EX28DRAFT_3723 at 400.006 kb on + strand, within EX28DRAFT_3723 at 400.007 kb on - strand, within EX28DRAFT_3723 at 400.215 kb on + strand at 400.238 kb on + strand at 400.238 kb on + strand at 400.238 kb on + strand at 400.238 kb on + strand at 400.238 kb on + strand at 400.238 kb on + strand at 400.239 kb on - strand at 400.239 kb on - strand at 400.239 kb on - strand at 400.239 kb on - strand at 400.239 kb on - strand at 400.242 kb on + strand at 400.242 kb on + strand at 400.242 kb on + strand at 400.242 kb on + strand at 400.242 kb on + strand at 400.243 kb on - strand at 400.243 kb on - strand at 400.243 kb on - strand at 400.243 kb on - strand at 400.243 kb on - strand at 400.243 kb on - strand at 400.243 kb on - strand at 400.243 kb on - strand at 400.280 kb on - strand at 400.293 kb on - strand at 400.295 kb on + strand at 400.296 kb on - strand at 400.297 kb on + strand at 400.297 kb on + strand at 400.350 kb on - strand at 400.354 kb on + strand at 400.354 kb on + strand at 400.390 kb on + strand at 400.431 kb on + strand at 400.432 kb on - strand at 400.460 kb on + strand, within EX28DRAFT_3724 at 400.690 kb on + strand, within EX28DRAFT_3724 at 400.723 kb on + strand, within EX28DRAFT_3724 at 401.103 kb on - strand, within EX28DRAFT_3724 at 401.108 kb on + strand, within EX28DRAFT_3724 at 401.164 kb on + strand, within EX28DRAFT_3724 at 401.208 kb on + strand at 401.208 kb on + strand at 401.212 kb on + strand at 401.219 kb on - strand at 401.259 kb on + strand at 401.260 kb on - strand at 401.344 kb on - strand at 401.349 kb on + strand at 401.350 kb on - strand at 401.395 kb on + strand, within EX28DRAFT_3725 at 401.396 kb on - strand, within EX28DRAFT_3725 at 401.451 kb on + strand, within EX28DRAFT_3725 at 401.451 kb on + strand, within EX28DRAFT_3725 at 401.452 kb on - strand, within EX28DRAFT_3725 at 401.452 kb on - strand, within EX28DRAFT_3725 at 401.452 kb on - strand, within EX28DRAFT_3725 at 401.455 kb on + strand, within EX28DRAFT_3725 at 401.455 kb on + strand, within EX28DRAFT_3725 at 401.455 kb on + strand, within EX28DRAFT_3725 at 401.455 kb on + strand, within EX28DRAFT_3725 at 401.456 kb on - strand, within EX28DRAFT_3725 at 401.456 kb on - strand, within EX28DRAFT_3725 at 401.456 kb on - strand, within EX28DRAFT_3725 at 401.457 kb on + strand, within EX28DRAFT_3725 at 401.458 kb on - strand, within EX28DRAFT_3725 at 401.519 kb on + strand, within EX28DRAFT_3725 at 401.519 kb on + strand, within EX28DRAFT_3725 at 401.520 kb on - strand, within EX28DRAFT_3725 at 401.849 kb on - strand, within EX28DRAFT_3725 at 401.908 kb on + strand, within EX28DRAFT_3725 at 401.908 kb on + strand, within EX28DRAFT_3725 at 401.909 kb on - strand, within EX28DRAFT_3725 at 401.992 kb on - strand at 402.005 kb on - strand at 402.102 kb on + strand at 402.147 kb on - strand at 402.158 kb on + strand at 402.158 kb on + strand at 402.159 kb on - strand at 402.159 kb on - strand at 402.159 kb on - strand at 402.159 kb on - strand at 402.212 kb on + strand, within EX28DRAFT_3726
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media no stress control remove 399,527 + +0.3 399,593 - +1.5 399,596 + +0.4 399,612 - -0.1 399,612 - +0.2 399,713 - EX28DRAFT_3723 0.18 -0.0 399,785 + EX28DRAFT_3723 0.29 -0.3 399,787 + EX28DRAFT_3723 0.30 -0.0 399,787 + EX28DRAFT_3723 0.30 +0.1 399,787 + EX28DRAFT_3723 0.30 -0.1 399,787 + EX28DRAFT_3723 0.30 -0.8 399,787 + EX28DRAFT_3723 0.30 +0.2 399,788 - EX28DRAFT_3723 0.30 +0.5 399,788 - EX28DRAFT_3723 0.30 -0.8 399,788 - EX28DRAFT_3723 0.30 -0.3 399,864 - EX28DRAFT_3723 0.41 +0.1 399,864 - EX28DRAFT_3723 0.41 -0.0 399,864 - EX28DRAFT_3723 0.41 -0.2 399,864 - EX28DRAFT_3723 0.41 -0.2 400,006 + EX28DRAFT_3723 0.63 +0.0 400,007 - EX28DRAFT_3723 0.63 +0.0 400,215 + -0.5 400,238 + -0.3 400,238 + +0.3 400,238 + +0.1 400,238 + -0.3 400,238 + -0.3 400,238 + +0.7 400,239 - +0.1 400,239 - -1.2 400,239 - -0.2 400,239 - -0.0 400,239 - +0.3 400,242 + -0.1 400,242 + -0.3 400,242 + +0.5 400,242 + -1.1 400,242 + -0.3 400,243 - +0.1 400,243 - +0.2 400,243 - -0.2 400,243 - +0.0 400,243 - +1.3 400,243 - -0.6 400,243 - -0.0 400,243 - -0.7 400,280 - +0.1 400,293 - +0.0 400,295 + +0.2 400,296 - +0.1 400,297 + +0.3 400,297 + -0.1 400,350 - -0.2 400,354 + +0.2 400,354 + -3.1 400,390 + -0.1 400,431 + -0.0 400,432 - +0.5 400,460 + EX28DRAFT_3724 0.11 -1.9 400,690 + EX28DRAFT_3724 0.35 -0.8 400,723 + EX28DRAFT_3724 0.39 -2.2 401,103 - EX28DRAFT_3724 0.80 -0.0 401,108 + EX28DRAFT_3724 0.81 +0.0 401,164 + EX28DRAFT_3724 0.87 -0.3 401,208 + +0.2 401,208 + +0.6 401,212 + -0.0 401,219 - +0.8 401,259 + +0.2 401,260 - +0.8 401,344 - -0.1 401,349 + +0.9 401,350 - +1.3 401,395 + EX28DRAFT_3725 0.14 +0.3 401,396 - EX28DRAFT_3725 0.15 -0.4 401,451 + EX28DRAFT_3725 0.22 +0.2 401,451 + EX28DRAFT_3725 0.22 +0.0 401,452 - EX28DRAFT_3725 0.22 +0.0 401,452 - EX28DRAFT_3725 0.22 -0.0 401,452 - EX28DRAFT_3725 0.22 +0.6 401,455 + EX28DRAFT_3725 0.22 -0.4 401,455 + EX28DRAFT_3725 0.22 -0.2 401,455 + EX28DRAFT_3725 0.22 +0.6 401,455 + EX28DRAFT_3725 0.22 -0.2 401,456 - EX28DRAFT_3725 0.22 -0.6 401,456 - EX28DRAFT_3725 0.22 -0.4 401,456 - EX28DRAFT_3725 0.22 +0.2 401,457 + EX28DRAFT_3725 0.22 -0.0 401,458 - EX28DRAFT_3725 0.23 +0.4 401,519 + EX28DRAFT_3725 0.30 +0.0 401,519 + EX28DRAFT_3725 0.30 -0.3 401,520 - EX28DRAFT_3725 0.31 -0.6 401,849 - EX28DRAFT_3725 0.73 +0.0 401,908 + EX28DRAFT_3725 0.81 -1.1 401,908 + EX28DRAFT_3725 0.81 +0.7 401,909 - EX28DRAFT_3725 0.81 +0.0 401,992 - +0.6 402,005 - +0.2 402,102 + -0.2 402,147 - -0.6 402,158 + -0.2 402,158 + +0.1 402,159 - -0.6 402,159 - +0.9 402,159 - -0.1 402,159 - +0.0 402,212 + EX28DRAFT_3726 0.12 +0.1
Or see this region's nucleotide sequence