Experiment: MS_media no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2874 and EX28DRAFT_2875 are separated by 218 nucleotides EX28DRAFT_2875 and EX28DRAFT_2876 are separated by 69 nucleotides EX28DRAFT_2876 and EX28DRAFT_2877 are separated by 116 nucleotides
EX28DRAFT_2874: EX28DRAFT_2874 - RNA, at 180,522 to 180,878
_2874
EX28DRAFT_2875: EX28DRAFT_2875 - Syd protein (SUKH-2), at 181,097 to 181,642
_2875
EX28DRAFT_2876: EX28DRAFT_2876 - 7-cyano-7-deazaguanine reductase, at 181,712 to 182,554
_2876
EX28DRAFT_2877: EX28DRAFT_2877 - Predicted Rossmann fold nucleotide-binding protein, at 182,671 to 184,035
_2877
Position (kb)
181
182
183 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 180.893 kb on + strand at 180.893 kb on + strand at 180.893 kb on + strand at 180.894 kb on - strand at 180.919 kb on - strand at 180.952 kb on - strand at 180.952 kb on - strand at 181.063 kb on - strand at 181.099 kb on - strand at 181.213 kb on + strand, within EX28DRAFT_2875 at 181.264 kb on + strand, within EX28DRAFT_2875 at 181.355 kb on - strand, within EX28DRAFT_2875 at 181.401 kb on + strand, within EX28DRAFT_2875 at 181.495 kb on + strand, within EX28DRAFT_2875 at 181.501 kb on - strand, within EX28DRAFT_2875 at 181.520 kb on - strand, within EX28DRAFT_2875 at 181.535 kb on + strand, within EX28DRAFT_2875 at 181.612 kb on + strand at 181.683 kb on + strand at 181.683 kb on + strand at 181.684 kb on - strand at 181.689 kb on + strand at 181.689 kb on + strand at 181.689 kb on + strand at 181.690 kb on - strand at 181.705 kb on - strand at 181.887 kb on - strand, within EX28DRAFT_2876 at 181.887 kb on - strand, within EX28DRAFT_2876 at 181.887 kb on - strand, within EX28DRAFT_2876 at 181.952 kb on + strand, within EX28DRAFT_2876 at 181.953 kb on - strand, within EX28DRAFT_2876 at 181.987 kb on + strand, within EX28DRAFT_2876 at 181.988 kb on - strand, within EX28DRAFT_2876 at 182.065 kb on - strand, within EX28DRAFT_2876 at 182.187 kb on - strand, within EX28DRAFT_2876 at 182.411 kb on - strand, within EX28DRAFT_2876 at 182.469 kb on - strand, within EX28DRAFT_2876 at 182.555 kb on + strand at 182.558 kb on - strand at 182.561 kb on + strand at 182.562 kb on - strand at 182.573 kb on - strand at 182.629 kb on - strand at 182.629 kb on - strand at 182.629 kb on - strand at 182.677 kb on - strand at 182.682 kb on + strand at 182.682 kb on + strand at 182.682 kb on + strand at 182.683 kb on - strand at 182.683 kb on - strand at 182.685 kb on + strand at 182.686 kb on - strand at 182.706 kb on + strand at 182.707 kb on - strand at 182.707 kb on - strand at 182.707 kb on - strand at 182.736 kb on + strand at 182.793 kb on - strand at 182.818 kb on - strand, within EX28DRAFT_2877 at 182.818 kb on - strand, within EX28DRAFT_2877 at 182.823 kb on + strand, within EX28DRAFT_2877 at 183.052 kb on - strand, within EX28DRAFT_2877 at 183.160 kb on + strand, within EX28DRAFT_2877 at 183.161 kb on - strand, within EX28DRAFT_2877 at 183.161 kb on - strand, within EX28DRAFT_2877 at 183.161 kb on - strand, within EX28DRAFT_2877 at 183.210 kb on + strand, within EX28DRAFT_2877 at 183.210 kb on + strand, within EX28DRAFT_2877 at 183.210 kb on + strand, within EX28DRAFT_2877 at 183.210 kb on + strand, within EX28DRAFT_2877 at 183.211 kb on - strand, within EX28DRAFT_2877 at 183.287 kb on - strand, within EX28DRAFT_2877 at 183.369 kb on + strand, within EX28DRAFT_2877 at 183.370 kb on - strand, within EX28DRAFT_2877 at 183.426 kb on + strand, within EX28DRAFT_2877 at 183.469 kb on + strand, within EX28DRAFT_2877 at 183.470 kb on - strand, within EX28DRAFT_2877 at 183.470 kb on - strand, within EX28DRAFT_2877 at 183.480 kb on + strand, within EX28DRAFT_2877 at 183.480 kb on + strand, within EX28DRAFT_2877 at 183.553 kb on + strand, within EX28DRAFT_2877
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media no stress control remove 180,893 + -0.2 180,893 + -0.1 180,893 + +0.1 180,894 - +0.1 180,919 - +0.2 180,952 - +0.8 180,952 - +0.3 181,063 - -0.2 181,099 - -0.4 181,213 + EX28DRAFT_2875 0.21 -0.2 181,264 + EX28DRAFT_2875 0.31 +1.0 181,355 - EX28DRAFT_2875 0.47 +0.5 181,401 + EX28DRAFT_2875 0.56 -0.2 181,495 + EX28DRAFT_2875 0.73 -0.7 181,501 - EX28DRAFT_2875 0.74 -0.1 181,520 - EX28DRAFT_2875 0.77 -0.2 181,535 + EX28DRAFT_2875 0.80 -0.1 181,612 + +0.7 181,683 + -1.8 181,683 + -0.6 181,684 - +0.3 181,689 + +0.7 181,689 + +0.6 181,689 + -1.0 181,690 - +0.3 181,705 - +0.3 181,887 - EX28DRAFT_2876 0.21 +0.0 181,887 - EX28DRAFT_2876 0.21 +0.2 181,887 - EX28DRAFT_2876 0.21 -0.0 181,952 + EX28DRAFT_2876 0.28 +0.5 181,953 - EX28DRAFT_2876 0.29 +0.0 181,987 + EX28DRAFT_2876 0.33 +0.7 181,988 - EX28DRAFT_2876 0.33 +0.4 182,065 - EX28DRAFT_2876 0.42 -0.1 182,187 - EX28DRAFT_2876 0.56 +0.0 182,411 - EX28DRAFT_2876 0.83 -1.1 182,469 - EX28DRAFT_2876 0.90 +0.1 182,555 + -0.1 182,558 - +0.3 182,561 + -0.2 182,562 - +0.7 182,573 - +0.0 182,629 - -0.1 182,629 - -0.1 182,629 - -0.1 182,677 - +0.2 182,682 + +0.5 182,682 + -1.0 182,682 + +0.2 182,683 - +0.1 182,683 - +1.0 182,685 + -0.3 182,686 - -0.4 182,706 + -0.3 182,707 - -0.2 182,707 - -0.1 182,707 - +0.8 182,736 + +1.8 182,793 - +0.2 182,818 - EX28DRAFT_2877 0.11 -0.3 182,818 - EX28DRAFT_2877 0.11 -0.4 182,823 + EX28DRAFT_2877 0.11 -1.1 183,052 - EX28DRAFT_2877 0.28 +0.0 183,160 + EX28DRAFT_2877 0.36 -0.1 183,161 - EX28DRAFT_2877 0.36 -0.0 183,161 - EX28DRAFT_2877 0.36 -0.1 183,161 - EX28DRAFT_2877 0.36 -0.8 183,210 + EX28DRAFT_2877 0.39 +0.1 183,210 + EX28DRAFT_2877 0.39 +0.2 183,210 + EX28DRAFT_2877 0.39 +0.2 183,210 + EX28DRAFT_2877 0.39 -0.1 183,211 - EX28DRAFT_2877 0.40 -0.6 183,287 - EX28DRAFT_2877 0.45 -0.3 183,369 + EX28DRAFT_2877 0.51 -0.1 183,370 - EX28DRAFT_2877 0.51 -2.5 183,426 + EX28DRAFT_2877 0.55 -0.4 183,469 + EX28DRAFT_2877 0.58 -0.4 183,470 - EX28DRAFT_2877 0.59 +0.2 183,470 - EX28DRAFT_2877 0.59 +0.2 183,480 + EX28DRAFT_2877 0.59 +2.7 183,480 + EX28DRAFT_2877 0.59 +0.2 183,553 + EX28DRAFT_2877 0.65 -0.2
Or see this region's nucleotide sequence