Experiment: MS_media no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1322 and EX28DRAFT_1323 are separated by 11 nucleotides EX28DRAFT_1323 and EX28DRAFT_1324 overlap by 4 nucleotides EX28DRAFT_1324 and EX28DRAFT_1325 overlap by 4 nucleotides EX28DRAFT_1325 and EX28DRAFT_1326 are separated by 118 nucleotides EX28DRAFT_1326 and EX28DRAFT_1327 are separated by 1 nucleotides
EX28DRAFT_1322: EX28DRAFT_1322 - hypothetical protein, at 1,321,871 to 1,322,581
_1322
EX28DRAFT_1323: EX28DRAFT_1323 - Protein of unknown function (DUF1317), at 1,322,593 to 1,322,745
_1323
EX28DRAFT_1324: EX28DRAFT_1324 - Methyltransferase domain, at 1,322,742 to 1,323,224
_1324
EX28DRAFT_1325: EX28DRAFT_1325 - Transcriptional activator, adenine-specific DNA methyltransferase, at 1,323,221 to 1,323,880
_1325
EX28DRAFT_1326: EX28DRAFT_1326 - transcriptional regulator, TraR/DksA family, at 1,323,999 to 1,324,214
_1326
EX28DRAFT_1327: EX28DRAFT_1327 - hypothetical protein, at 1,324,216 to 1,324,740
_1327
Position (kb)
1323
1324 Strain fitness (log2 ratio)
-2
-1
0
1 at 1322.222 kb on - strand, within EX28DRAFT_1322 at 1322.282 kb on + strand, within EX28DRAFT_1322 at 1322.282 kb on + strand, within EX28DRAFT_1322 at 1322.330 kb on + strand, within EX28DRAFT_1322 at 1322.331 kb on - strand, within EX28DRAFT_1322 at 1322.332 kb on + strand, within EX28DRAFT_1322 at 1322.474 kb on - strand, within EX28DRAFT_1322 at 1322.569 kb on + strand at 1322.592 kb on + strand at 1322.593 kb on - strand at 1322.649 kb on + strand, within EX28DRAFT_1323 at 1322.875 kb on + strand, within EX28DRAFT_1324 at 1322.888 kb on + strand, within EX28DRAFT_1324 at 1322.889 kb on - strand, within EX28DRAFT_1324 at 1322.890 kb on + strand, within EX28DRAFT_1324 at 1323.125 kb on + strand, within EX28DRAFT_1324 at 1323.158 kb on - strand, within EX28DRAFT_1324 at 1323.241 kb on + strand at 1323.241 kb on + strand at 1323.245 kb on + strand at 1323.246 kb on - strand at 1323.267 kb on - strand at 1323.386 kb on + strand, within EX28DRAFT_1325 at 1323.665 kb on - strand, within EX28DRAFT_1325 at 1323.672 kb on + strand, within EX28DRAFT_1325 at 1323.676 kb on - strand, within EX28DRAFT_1325 at 1323.676 kb on - strand, within EX28DRAFT_1325 at 1323.676 kb on - strand, within EX28DRAFT_1325 at 1323.676 kb on - strand, within EX28DRAFT_1325 at 1323.677 kb on + strand, within EX28DRAFT_1325 at 1323.678 kb on - strand, within EX28DRAFT_1325 at 1323.678 kb on - strand, within EX28DRAFT_1325 at 1323.678 kb on - strand, within EX28DRAFT_1325 at 1323.915 kb on - strand at 1323.947 kb on + strand at 1323.973 kb on - strand at 1324.213 kb on - strand at 1324.236 kb on - strand at 1324.302 kb on - strand, within EX28DRAFT_1327 at 1324.323 kb on - strand, within EX28DRAFT_1327 at 1324.340 kb on - strand, within EX28DRAFT_1327 at 1324.347 kb on - strand, within EX28DRAFT_1327 at 1324.363 kb on - strand, within EX28DRAFT_1327 at 1324.405 kb on + strand, within EX28DRAFT_1327 at 1324.422 kb on - strand, within EX28DRAFT_1327 at 1324.422 kb on - strand, within EX28DRAFT_1327 at 1324.440 kb on + strand, within EX28DRAFT_1327 at 1324.440 kb on + strand, within EX28DRAFT_1327 at 1324.441 kb on - strand, within EX28DRAFT_1327 at 1324.474 kb on - strand, within EX28DRAFT_1327 at 1324.479 kb on + strand, within EX28DRAFT_1327 at 1324.480 kb on - strand, within EX28DRAFT_1327 at 1324.480 kb on - strand, within EX28DRAFT_1327 at 1324.484 kb on - strand, within EX28DRAFT_1327 at 1324.558 kb on + strand, within EX28DRAFT_1327 at 1324.558 kb on + strand, within EX28DRAFT_1327 at 1324.621 kb on + strand, within EX28DRAFT_1327 at 1324.622 kb on - strand, within EX28DRAFT_1327 at 1324.656 kb on + strand, within EX28DRAFT_1327 at 1324.855 kb on + strand at 1324.855 kb on + strand at 1324.857 kb on + strand at 1324.857 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media no stress control remove 1,322,222 - EX28DRAFT_1322 0.49 +0.5 1,322,282 + EX28DRAFT_1322 0.58 -0.9 1,322,282 + EX28DRAFT_1322 0.58 -0.2 1,322,330 + EX28DRAFT_1322 0.65 -0.2 1,322,331 - EX28DRAFT_1322 0.65 +0.2 1,322,332 + EX28DRAFT_1322 0.65 +0.1 1,322,474 - EX28DRAFT_1322 0.85 +0.8 1,322,569 + +0.1 1,322,592 + -0.8 1,322,593 - +0.2 1,322,649 + EX28DRAFT_1323 0.37 +0.0 1,322,875 + EX28DRAFT_1324 0.28 -0.4 1,322,888 + EX28DRAFT_1324 0.30 -0.0 1,322,889 - EX28DRAFT_1324 0.30 +0.2 1,322,890 + EX28DRAFT_1324 0.31 +0.5 1,323,125 + EX28DRAFT_1324 0.79 +0.2 1,323,158 - EX28DRAFT_1324 0.86 +0.1 1,323,241 + -0.0 1,323,241 + +0.6 1,323,245 + -0.3 1,323,246 - -0.3 1,323,267 - +0.1 1,323,386 + EX28DRAFT_1325 0.25 +0.3 1,323,665 - EX28DRAFT_1325 0.67 -1.9 1,323,672 + EX28DRAFT_1325 0.68 +0.1 1,323,676 - EX28DRAFT_1325 0.69 -0.1 1,323,676 - EX28DRAFT_1325 0.69 -0.9 1,323,676 - EX28DRAFT_1325 0.69 -0.0 1,323,676 - EX28DRAFT_1325 0.69 +0.8 1,323,677 + EX28DRAFT_1325 0.69 -0.7 1,323,678 - EX28DRAFT_1325 0.69 +0.3 1,323,678 - EX28DRAFT_1325 0.69 -0.5 1,323,678 - EX28DRAFT_1325 0.69 +0.7 1,323,915 - +0.4 1,323,947 + +0.8 1,323,973 - -0.8 1,324,213 - +1.1 1,324,236 - +0.3 1,324,302 - EX28DRAFT_1327 0.16 +0.5 1,324,323 - EX28DRAFT_1327 0.20 -0.4 1,324,340 - EX28DRAFT_1327 0.24 -1.3 1,324,347 - EX28DRAFT_1327 0.25 -0.1 1,324,363 - EX28DRAFT_1327 0.28 -0.2 1,324,405 + EX28DRAFT_1327 0.36 -0.2 1,324,422 - EX28DRAFT_1327 0.39 +0.4 1,324,422 - EX28DRAFT_1327 0.39 +0.5 1,324,440 + EX28DRAFT_1327 0.43 -0.2 1,324,440 + EX28DRAFT_1327 0.43 -0.0 1,324,441 - EX28DRAFT_1327 0.43 +0.1 1,324,474 - EX28DRAFT_1327 0.49 +0.3 1,324,479 + EX28DRAFT_1327 0.50 +0.5 1,324,480 - EX28DRAFT_1327 0.50 -0.4 1,324,480 - EX28DRAFT_1327 0.50 -0.2 1,324,484 - EX28DRAFT_1327 0.51 -1.6 1,324,558 + EX28DRAFT_1327 0.65 +0.1 1,324,558 + EX28DRAFT_1327 0.65 +0.6 1,324,621 + EX28DRAFT_1327 0.77 -0.6 1,324,622 - EX28DRAFT_1327 0.77 +0.6 1,324,656 + EX28DRAFT_1327 0.84 -0.3 1,324,855 + -0.3 1,324,855 + +0.1 1,324,857 + -0.2 1,324,857 + -0.4
Or see this region's nucleotide sequence