Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS02660 and ABID97_RS02665 overlap by 4 nucleotides ABID97_RS02665 and ABID97_RS02670 overlap by 4 nucleotides ABID97_RS02670 and ABID97_RS02675 are separated by 98 nucleotides ABID97_RS02675 and ABID97_RS02680 are separated by 320 nucleotides
ABID97_RS02660: ABID97_RS02660 - membrane protein insertase YidC, at 542,527 to 544,212
_RS02660
ABID97_RS02665: ABID97_RS02665 - membrane protein insertion efficiency factor YidD, at 544,209 to 544,496
_RS02665
ABID97_RS02670: ABID97_RS02670 - ribonuclease P protein component, at 544,493 to 544,885
_RS02670
ABID97_RS02675: ABID97_RS02675 - 50S ribosomal protein L34, at 544,984 to 545,118
_RS02675
ABID97_RS02680: ABID97_RS02680 - chromosomal replication initiator protein DnaA, at 545,439 to 546,818
_RS02680
Position (kb) 544
545 Strain fitness (log2 ratio) -1
0
1
2 at 544.354 kb on + strand, within ABID97_RS02665 at 544.355 kb on - strand, within ABID97_RS02665 at 544.355 kb on - strand, within ABID97_RS02665 at 544.355 kb on - strand, within ABID97_RS02665 at 544.355 kb on - strand, within ABID97_RS02665 at 544.400 kb on - strand, within ABID97_RS02665 at 545.352 kb on + strand at 545.390 kb on + strand at 545.390 kb on + strand at 545.390 kb on + strand at 545.390 kb on + strand at 545.390 kb on + strand at 545.390 kb on + strand at 545.390 kb on + strand at 545.394 kb on + strand at 545.394 kb on + strand at 545.394 kb on + strand at 545.395 kb on - strand at 545.395 kb on - strand at 545.395 kb on - strand at 545.396 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 544,354 + ABID97_RS02665 0.50 +1.7 544,355 - ABID97_RS02665 0.51 +0.7 544,355 - ABID97_RS02665 0.51 +0.1 544,355 - ABID97_RS02665 0.51 +0.3 544,355 - ABID97_RS02665 0.51 +0.4 544,400 - ABID97_RS02665 0.66 +0.3 545,352 + -0.3 545,390 + -1.5 545,390 + +0.7 545,390 + -0.4 545,390 + +0.5 545,390 + +2.1 545,390 + +2.3 545,390 + +0.5 545,394 + -1.3 545,394 + +0.4 545,394 + -1.3 545,395 - -0.9 545,395 - -0.7 545,395 - +0.5 545,396 + +0.1
Or see this region's nucleotide sequence