Strain Fitness in Escherichia coli BW25113 around b2134

Experiment: D-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdld and pbpG are separated by 37 nucleotidespbpG and yohC are separated by 173 nucleotidesyohC and yohD are separated by 169 nucleotides b2133: dld - D-lactate dehydrogenase, FAD-binding, NADH independent (NCBI), at 2,220,207 to 2,221,922 dld b2134: pbpG - penicillin-binding protein 7 (VIMSS), at 2,221,960 to 2,222,892 pbpG b2135: yohC - orf, hypothetical protein (VIMSS), at 2,223,066 to 2,223,653 yohC b2136: yohD - orf, hypothetical protein (VIMSS), at 2,223,823 to 2,224,401 yohD Position (kb) 2221 2222 2223Strain fitness (log2 ratio) -2 -1 0 1 2at 2221.087 kb on + strand, within dldat 2221.199 kb on + strand, within dldat 2221.199 kb on + strand, within dldat 2221.333 kb on + strand, within dldat 2221.333 kb on + strand, within dldat 2221.373 kb on + strand, within dldat 2221.373 kb on + strand, within dldat 2221.482 kb on - strand, within dldat 2221.505 kb on - strand, within dldat 2221.577 kb on + strand, within dldat 2221.658 kb on - strand, within dldat 2221.687 kb on + strand, within dldat 2221.697 kb on + strand, within dldat 2221.697 kb on + strand, within dldat 2221.730 kb on - strand, within dldat 2221.780 kb on + strandat 2221.781 kb on - strandat 2221.938 kb on - strandat 2222.020 kb on + strandat 2222.020 kb on + strandat 2222.154 kb on + strand, within pbpGat 2222.163 kb on + strand, within pbpGat 2222.171 kb on - strand, within pbpGat 2222.183 kb on + strand, within pbpGat 2222.254 kb on - strand, within pbpGat 2222.306 kb on + strand, within pbpGat 2222.314 kb on - strand, within pbpGat 2222.314 kb on - strand, within pbpGat 2222.314 kb on - strand, within pbpGat 2222.314 kb on - strand, within pbpGat 2222.552 kb on - strand, within pbpGat 2222.552 kb on - strand, within pbpGat 2222.602 kb on - strand, within pbpGat 2222.804 kb on - strandat 2222.806 kb on - strandat 2222.976 kb on - strandat 2223.193 kb on - strand, within yohCat 2223.323 kb on - strand, within yohCat 2223.457 kb on - strand, within yohCat 2223.476 kb on - strand, within yohCat 2223.531 kb on + strand, within yohCat 2223.573 kb on - strand, within yohCat 2223.647 kb on + strandat 2223.831 kb on + strandat 2223.879 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Lactate (C)
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2,221,087 + dld b2133 0.51 +0.3
2,221,199 + dld b2133 0.58 -0.1
2,221,199 + dld b2133 0.58 -1.4
2,221,333 + dld b2133 0.66 -2.1
2,221,333 + dld b2133 0.66 +0.0
2,221,373 + dld b2133 0.68 -1.6
2,221,373 + dld b2133 0.68 -1.5
2,221,482 - dld b2133 0.74 -0.7
2,221,505 - dld b2133 0.76 -2.5
2,221,577 + dld b2133 0.80 -1.5
2,221,658 - dld b2133 0.85 -1.8
2,221,687 + dld b2133 0.86 +0.2
2,221,697 + dld b2133 0.87 -1.6
2,221,697 + dld b2133 0.87 -0.1
2,221,730 - dld b2133 0.89 -1.4
2,221,780 + -0.9
2,221,781 - -1.2
2,221,938 - -1.1
2,222,020 + +0.3
2,222,020 + -0.4
2,222,154 + pbpG b2134 0.21 +0.2
2,222,163 + pbpG b2134 0.22 +0.4
2,222,171 - pbpG b2134 0.23 -0.1
2,222,183 + pbpG b2134 0.24 +0.5
2,222,254 - pbpG b2134 0.32 +0.7
2,222,306 + pbpG b2134 0.37 +0.5
2,222,314 - pbpG b2134 0.38 +0.5
2,222,314 - pbpG b2134 0.38 -0.2
2,222,314 - pbpG b2134 0.38 -0.6
2,222,314 - pbpG b2134 0.38 +2.6
2,222,552 - pbpG b2134 0.63 +0.6
2,222,552 - pbpG b2134 0.63 +0.4
2,222,602 - pbpG b2134 0.69 -0.0
2,222,804 - +0.9
2,222,806 - +0.9
2,222,976 - -1.1
2,223,193 - yohC b2135 0.22 -0.3
2,223,323 - yohC b2135 0.44 +0.4
2,223,457 - yohC b2135 0.66 +0.1
2,223,476 - yohC b2135 0.70 +0.5
2,223,531 + yohC b2135 0.79 -0.6
2,223,573 - yohC b2135 0.86 -0.3
2,223,647 + +0.6
2,223,831 + +0.5
2,223,879 + +0.0

Or see this region's nucleotide sequence