Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybiW and ybiY are separated by 5 nucleotides ybiY and mipB are separated by 130 nucleotides mipB and moeB are separated by 75 nucleotides
b0823: ybiW - predicted pyruvate formate lyase (NCBI), at 859,397 to 861,829
ybiW
b0824: ybiY - predicted pyruvate formate lyase activating enzyme (RefSeq), at 861,835 to 862,734
ybiY
b0825: mipB - putative transaldolase (VIMSS), at 862,865 to 863,527
mipB
b0826: moeB - molybdopterin biosynthesis protein MoeB (NCBI), at 863,603 to 864,352
moeB
Position (kb) 861
862
863 Strain fitness (log2 ratio) -1
0
1 at 860.884 kb on - strand, within ybiW at 861.029 kb on - strand, within ybiW at 861.287 kb on - strand, within ybiW at 861.428 kb on + strand, within ybiW at 861.428 kb on + strand, within ybiW at 861.428 kb on + strand, within ybiW at 861.715 kb on + strand at 861.715 kb on + strand at 861.723 kb on - strand at 861.728 kb on - strand at 861.749 kb on - strand at 861.947 kb on - strand, within ybiY at 862.001 kb on + strand, within ybiY at 862.063 kb on - strand, within ybiY at 862.219 kb on - strand, within ybiY at 862.295 kb on + strand, within ybiY at 862.398 kb on - strand, within ybiY at 862.435 kb on + strand, within ybiY at 862.435 kb on + strand, within ybiY at 862.439 kb on - strand, within ybiY at 862.442 kb on + strand, within ybiY at 862.442 kb on + strand, within ybiY at 862.462 kb on + strand, within ybiY at 862.575 kb on + strand, within ybiY at 862.575 kb on + strand, within ybiY at 862.580 kb on + strand, within ybiY at 862.600 kb on + strand, within ybiY at 862.697 kb on - strand at 862.717 kb on - strand at 862.828 kb on + strand at 863.057 kb on + strand, within mipB at 863.251 kb on + strand, within mipB at 863.433 kb on + strand, within mipB at 863.554 kb on + strand at 863.625 kb on - strand at 863.673 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 860,884 - ybiW b0823 0.61 -1.3 861,029 - ybiW b0823 0.67 +0.1 861,287 - ybiW b0823 0.78 -1.5 861,428 + ybiW b0823 0.83 -0.5 861,428 + ybiW b0823 0.83 -0.4 861,428 + ybiW b0823 0.83 +0.3 861,715 + -0.6 861,715 + -1.5 861,723 - +0.4 861,728 - +0.5 861,749 - +0.2 861,947 - ybiY b0824 0.12 -0.5 862,001 + ybiY b0824 0.18 -0.2 862,063 - ybiY b0824 0.25 +0.6 862,219 - ybiY b0824 0.43 -0.1 862,295 + ybiY b0824 0.51 +0.7 862,398 - ybiY b0824 0.63 -0.4 862,435 + ybiY b0824 0.67 -0.1 862,435 + ybiY b0824 0.67 +0.5 862,439 - ybiY b0824 0.67 -0.2 862,442 + ybiY b0824 0.67 -0.0 862,442 + ybiY b0824 0.67 -0.1 862,462 + ybiY b0824 0.70 -0.4 862,575 + ybiY b0824 0.82 +0.7 862,575 + ybiY b0824 0.82 -0.1 862,580 + ybiY b0824 0.83 -0.9 862,600 + ybiY b0824 0.85 -0.6 862,697 - -0.7 862,717 - -0.8 862,828 + +0.5 863,057 + mipB b0825 0.29 +0.1 863,251 + mipB b0825 0.58 +0.7 863,433 + mipB b0825 0.86 +0.6 863,554 + -0.7 863,625 - +0.2 863,673 - +0.5
Or see this region's nucleotide sequence