Strain Fitness in Escherichia coli BW25113 around b4068

Experiment: D-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntactP and yjcH overlap by 4 nucleotidesyjcH and acs are separated by 199 nucleotides b4067: actP - acetate permease (NCBI), at 4,281,276 to 4,282,925 actP b4068: yjcH - required for glycolate transport, with actP (from data), at 4,282,922 to 4,283,236 yjcH b4069: acs - acetyl-coenzyme A synthetase (NCBI), at 4,283,436 to 4,285,394 acs Position (kb) 4282 4283 4284Strain fitness (log2 ratio) -1 0 1 2at 4281.927 kb on - strand, within actPat 4281.934 kb on + strand, within actPat 4282.003 kb on - strand, within actPat 4282.033 kb on - strand, within actPat 4282.072 kb on - strand, within actPat 4282.094 kb on - strand, within actPat 4282.191 kb on - strand, within actPat 4282.191 kb on - strand, within actPat 4282.215 kb on - strand, within actPat 4282.215 kb on - strand, within actPat 4282.412 kb on - strand, within actPat 4282.531 kb on + strand, within actPat 4282.706 kb on - strand, within actPat 4282.718 kb on - strand, within actPat 4282.718 kb on - strand, within actPat 4282.738 kb on - strand, within actPat 4282.738 kb on - strand, within actPat 4282.740 kb on - strand, within actPat 4282.842 kb on + strandat 4282.864 kb on - strandat 4282.873 kb on - strandat 4282.909 kb on + strandat 4282.938 kb on - strandat 4282.990 kb on + strand, within yjcHat 4283.005 kb on + strand, within yjcHat 4283.013 kb on - strand, within yjcHat 4283.013 kb on - strand, within yjcHat 4283.013 kb on - strand, within yjcHat 4283.050 kb on + strand, within yjcHat 4283.121 kb on - strand, within yjcHat 4283.212 kb on - strandat 4283.212 kb on - strandat 4283.218 kb on + strandat 4283.224 kb on - strandat 4283.224 kb on - strandat 4283.291 kb on - strandat 4283.315 kb on - strandat 4283.315 kb on - strandat 4283.395 kb on + strandat 4283.395 kb on + strandat 4283.423 kb on + strandat 4283.423 kb on + strandat 4283.514 kb on + strandat 4283.514 kb on + strandat 4283.595 kb on - strandat 4283.678 kb on - strand, within acsat 4283.678 kb on - strand, within acsat 4283.712 kb on + strand, within acsat 4283.749 kb on - strand, within acsat 4283.808 kb on + strand, within acsat 4283.855 kb on + strand, within acsat 4283.855 kb on + strand, within acsat 4283.865 kb on + strand, within acsat 4283.956 kb on + strand, within acsat 4283.974 kb on - strand, within acsat 4283.981 kb on - strand, within acsat 4283.981 kb on - strand, within acsat 4283.983 kb on + strand, within acsat 4284.007 kb on - strand, within acsat 4284.032 kb on - strand, within acsat 4284.032 kb on - strand, within acsat 4284.215 kb on + strand, within acsat 4284.215 kb on + strand, within acsat 4284.229 kb on - strand, within acsat 4284.233 kb on + strand, within acs

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Lactate (C)
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4,281,927 - actP b4067 0.39 +2.3
4,281,934 + actP b4067 0.40 +0.0
4,282,003 - actP b4067 0.44 +0.1
4,282,033 - actP b4067 0.46 +0.1
4,282,072 - actP b4067 0.48 -0.7
4,282,094 - actP b4067 0.50 -0.1
4,282,191 - actP b4067 0.55 -0.4
4,282,191 - actP b4067 0.55 -0.1
4,282,215 - actP b4067 0.57 -0.9
4,282,215 - actP b4067 0.57 +0.3
4,282,412 - actP b4067 0.69 -0.5
4,282,531 + actP b4067 0.76 -1.3
4,282,706 - actP b4067 0.87 +0.0
4,282,718 - actP b4067 0.87 +0.1
4,282,718 - actP b4067 0.87 +0.5
4,282,738 - actP b4067 0.89 -0.1
4,282,738 - actP b4067 0.89 -0.1
4,282,740 - actP b4067 0.89 -0.5
4,282,842 + +0.7
4,282,864 - -0.6
4,282,873 - +0.7
4,282,909 + -1.2
4,282,938 - +0.5
4,282,990 + yjcH b4068 0.22 -1.3
4,283,005 + yjcH b4068 0.26 +0.1
4,283,013 - yjcH b4068 0.29 -0.9
4,283,013 - yjcH b4068 0.29 -0.1
4,283,013 - yjcH b4068 0.29 +0.3
4,283,050 + yjcH b4068 0.41 -0.0
4,283,121 - yjcH b4068 0.63 -0.1
4,283,212 - +1.0
4,283,212 - -0.2
4,283,218 + +0.1
4,283,224 - -0.1
4,283,224 - -0.8
4,283,291 - -0.0
4,283,315 - -1.1
4,283,315 - +0.2
4,283,395 + -0.3
4,283,395 + -0.2
4,283,423 + -0.2
4,283,423 + +0.3
4,283,514 + -0.3
4,283,514 + -1.0
4,283,595 - -0.1
4,283,678 - acs b4069 0.12 +0.3
4,283,678 - acs b4069 0.12 +1.2
4,283,712 + acs b4069 0.14 -1.6
4,283,749 - acs b4069 0.16 -0.2
4,283,808 + acs b4069 0.19 -0.3
4,283,855 + acs b4069 0.21 +0.4
4,283,855 + acs b4069 0.21 +0.2
4,283,865 + acs b4069 0.22 -0.1
4,283,956 + acs b4069 0.27 -1.0
4,283,974 - acs b4069 0.27 -0.7
4,283,981 - acs b4069 0.28 +0.2
4,283,981 - acs b4069 0.28 -0.3
4,283,983 + acs b4069 0.28 -0.2
4,284,007 - acs b4069 0.29 -0.5
4,284,032 - acs b4069 0.30 -0.7
4,284,032 - acs b4069 0.30 -0.4
4,284,215 + acs b4069 0.40 -1.1
4,284,215 + acs b4069 0.40 -0.7
4,284,229 - acs b4069 0.40 +0.4
4,284,233 + acs b4069 0.41 +0.4

Or see this region's nucleotide sequence