Strain Fitness in Escherichia coli BW25113 around b3223

Experiment: D-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyhcH and yhcI overlap by 4 nucleotidesyhcI and nanE overlap by 4 nucleotidesnanE and nanT are separated by 47 nucleotides b3221: yhcH - hypothetical protein (NCBI), at 3,367,036 to 3,367,500 yhcH b3222: yhcI - putative NAGC-like transcriptional regulator (VIMSS), at 3,367,497 to 3,368,372 yhcI b3223: nanE - predicted N-acetylmannosamine-6-P epimerase (NCBI), at 3,368,369 to 3,369,058 nanE b3224: nanT - sialic acid transporter (VIMSS), at 3,369,106 to 3,370,596 nanT Position (kb) 3368 3369 3370Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3367.386 kb on - strand, within yhcHat 3367.386 kb on - strand, within yhcHat 3367.444 kb on - strand, within yhcHat 3367.452 kb on - strand, within yhcHat 3367.454 kb on + strandat 3367.480 kb on - strandat 3367.480 kb on - strandat 3367.489 kb on + strandat 3367.523 kb on - strandat 3367.600 kb on + strand, within yhcIat 3367.600 kb on + strand, within yhcIat 3367.666 kb on - strand, within yhcIat 3367.678 kb on + strand, within yhcIat 3367.678 kb on + strand, within yhcIat 3367.691 kb on + strand, within yhcIat 3367.691 kb on + strand, within yhcIat 3367.725 kb on + strand, within yhcIat 3367.728 kb on + strand, within yhcIat 3367.768 kb on - strand, within yhcIat 3367.855 kb on - strand, within yhcIat 3367.855 kb on - strand, within yhcIat 3367.901 kb on + strand, within yhcIat 3368.002 kb on + strand, within yhcIat 3368.179 kb on - strand, within yhcIat 3368.179 kb on - strand, within yhcIat 3368.184 kb on - strand, within yhcIat 3368.285 kb on + strandat 3368.327 kb on - strandat 3368.368 kb on + strandat 3368.585 kb on + strand, within nanEat 3368.593 kb on - strand, within nanEat 3368.696 kb on - strand, within nanEat 3368.699 kb on + strand, within nanEat 3368.699 kb on + strand, within nanEat 3368.703 kb on + strand, within nanEat 3368.768 kb on + strand, within nanEat 3368.780 kb on + strand, within nanEat 3368.791 kb on + strand, within nanEat 3368.791 kb on + strand, within nanEat 3368.887 kb on + strand, within nanEat 3368.989 kb on - strand, within nanEat 3368.989 kb on - strand, within nanEat 3369.084 kb on + strandat 3369.297 kb on + strand, within nanTat 3369.480 kb on + strand, within nanTat 3369.488 kb on - strand, within nanTat 3369.488 kb on - strand, within nanTat 3369.538 kb on - strand, within nanTat 3369.640 kb on + strand, within nanTat 3369.669 kb on + strand, within nanTat 3369.791 kb on - strand, within nanTat 3369.793 kb on + strand, within nanTat 3369.831 kb on - strand, within nanTat 3369.941 kb on + strand, within nanTat 3370.043 kb on - strand, within nanT

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Lactate (C)
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3,367,386 - yhcH b3221 0.75 -0.3
3,367,386 - yhcH b3221 0.75 +0.4
3,367,444 - yhcH b3221 0.88 -0.8
3,367,452 - yhcH b3221 0.89 -0.7
3,367,454 + -0.9
3,367,480 - -0.4
3,367,480 - +0.1
3,367,489 + -1.2
3,367,523 - +0.6
3,367,600 + yhcI b3222 0.12 +0.1
3,367,600 + yhcI b3222 0.12 -1.7
3,367,666 - yhcI b3222 0.19 +0.8
3,367,678 + yhcI b3222 0.21 +0.5
3,367,678 + yhcI b3222 0.21 -0.3
3,367,691 + yhcI b3222 0.22 +0.5
3,367,691 + yhcI b3222 0.22 -1.2
3,367,725 + yhcI b3222 0.26 +0.3
3,367,728 + yhcI b3222 0.26 -0.2
3,367,768 - yhcI b3222 0.31 -1.4
3,367,855 - yhcI b3222 0.41 +1.0
3,367,855 - yhcI b3222 0.41 -0.8
3,367,901 + yhcI b3222 0.46 -0.5
3,368,002 + yhcI b3222 0.58 +0.5
3,368,179 - yhcI b3222 0.78 +0.5
3,368,179 - yhcI b3222 0.78 +1.1
3,368,184 - yhcI b3222 0.78 -1.2
3,368,285 + +2.2
3,368,327 - -0.4
3,368,368 + +0.3
3,368,585 + nanE b3223 0.31 -0.1
3,368,593 - nanE b3223 0.32 -0.9
3,368,696 - nanE b3223 0.47 +1.0
3,368,699 + nanE b3223 0.48 +0.3
3,368,699 + nanE b3223 0.48 +0.1
3,368,703 + nanE b3223 0.48 -0.3
3,368,768 + nanE b3223 0.58 -1.8
3,368,780 + nanE b3223 0.60 -0.6
3,368,791 + nanE b3223 0.61 +0.1
3,368,791 + nanE b3223 0.61 -0.4
3,368,887 + nanE b3223 0.75 +0.0
3,368,989 - nanE b3223 0.90 +0.1
3,368,989 - nanE b3223 0.90 +0.6
3,369,084 + -3.5
3,369,297 + nanT b3224 0.13 -0.1
3,369,480 + nanT b3224 0.25 -0.9
3,369,488 - nanT b3224 0.26 +0.1
3,369,488 - nanT b3224 0.26 +1.7
3,369,538 - nanT b3224 0.29 +0.1
3,369,640 + nanT b3224 0.36 -0.2
3,369,669 + nanT b3224 0.38 +0.1
3,369,791 - nanT b3224 0.46 +1.8
3,369,793 + nanT b3224 0.46 -0.4
3,369,831 - nanT b3224 0.49 -1.1
3,369,941 + nanT b3224 0.56 -0.2
3,370,043 - nanT b3224 0.63 +0.0

Or see this region's nucleotide sequence