Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ypfN and ypfH are separated by 109 nucleotides ypfH and ypfI are separated by 73 nucleotides ypfI and ypfJ are separated by 14 nucleotides
b4547: ypfN - hypothetical protein (NCBI), at 2,590,784 to 2,590,984
ypfN
b2473: ypfH - orf, hypothetical protein (VIMSS), at 2,591,094 to 2,591,792
ypfH
b2474: ypfI - predicted hydrolase (NCBI), at 2,591,866 to 2,593,881
ypfI
b2475: ypfJ - hypothetical protein (NCBI), at 2,593,896 to 2,594,759
ypfJ
Position (kb)
2591
2592
2593
2594 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2590.934 kb on + strand, within ypfN at 2590.934 kb on + strand, within ypfN at 2590.954 kb on - strand, within ypfN at 2590.954 kb on - strand, within ypfN at 2590.997 kb on + strand at 2591.119 kb on - strand at 2591.119 kb on - strand at 2591.127 kb on - strand at 2591.178 kb on - strand, within ypfH at 2591.178 kb on - strand, within ypfH at 2591.358 kb on - strand, within ypfH at 2591.372 kb on + strand, within ypfH at 2591.372 kb on + strand, within ypfH at 2591.380 kb on - strand, within ypfH at 2591.489 kb on + strand, within ypfH at 2591.494 kb on + strand, within ypfH at 2591.584 kb on - strand, within ypfH at 2591.584 kb on - strand, within ypfH at 2591.680 kb on - strand, within ypfH at 2591.720 kb on - strand, within ypfH at 2591.720 kb on - strand, within ypfH at 2591.752 kb on + strand at 2591.912 kb on - strand at 2591.978 kb on - strand at 2591.978 kb on - strand at 2591.999 kb on - strand at 2592.010 kb on - strand at 2592.105 kb on - strand, within ypfI at 2592.105 kb on - strand, within ypfI at 2592.141 kb on + strand, within ypfI at 2592.292 kb on - strand, within ypfI at 2592.292 kb on - strand, within ypfI at 2592.385 kb on + strand, within ypfI at 2592.385 kb on + strand, within ypfI at 2592.426 kb on + strand, within ypfI at 2592.448 kb on + strand, within ypfI at 2592.471 kb on - strand, within ypfI at 2592.531 kb on - strand, within ypfI at 2592.531 kb on - strand, within ypfI at 2592.535 kb on - strand, within ypfI at 2592.557 kb on + strand, within ypfI at 2592.576 kb on - strand, within ypfI at 2592.576 kb on - strand, within ypfI at 2592.581 kb on - strand, within ypfI at 2592.581 kb on - strand, within ypfI at 2592.751 kb on - strand, within ypfI at 2592.784 kb on - strand, within ypfI at 2592.942 kb on + strand, within ypfI at 2592.942 kb on + strand, within ypfI at 2592.942 kb on + strand, within ypfI at 2593.020 kb on - strand, within ypfI at 2593.303 kb on + strand, within ypfI at 2593.380 kb on - strand, within ypfI at 2593.380 kb on - strand, within ypfI at 2593.380 kb on - strand, within ypfI at 2593.394 kb on - strand, within ypfI at 2593.424 kb on - strand, within ypfI at 2593.424 kb on - strand, within ypfI at 2593.509 kb on - strand, within ypfI at 2593.509 kb on - strand, within ypfI at 2593.767 kb on + strand at 2593.775 kb on - strand at 2593.775 kb on - strand at 2593.960 kb on - strand at 2593.973 kb on + strand at 2594.113 kb on - strand, within ypfJ at 2594.113 kb on - strand, within ypfJ at 2594.224 kb on + strand, within ypfJ at 2594.224 kb on + strand, within ypfJ at 2594.282 kb on - strand, within ypfJ at 2594.282 kb on - strand, within ypfJ at 2594.331 kb on - strand, within ypfJ at 2594.338 kb on + strand, within ypfJ at 2594.375 kb on + strand, within ypfJ at 2594.385 kb on - strand, within ypfJ at 2594.430 kb on - strand, within ypfJ at 2594.449 kb on + strand, within ypfJ at 2594.449 kb on + strand, within ypfJ at 2594.449 kb on + strand, within ypfJ at 2594.449 kb on - strand, within ypfJ at 2594.540 kb on + strand, within ypfJ at 2594.573 kb on + strand, within ypfJ at 2594.608 kb on - strand, within ypfJ at 2594.720 kb on + strand at 2594.872 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 2,590,934 + ypfN b4547 0.75 -2.0 2,590,934 + ypfN b4547 0.75 -1.8 2,590,954 - ypfN b4547 0.85 -1.7 2,590,954 - ypfN b4547 0.85 -0.9 2,590,997 + +0.1 2,591,119 - -0.9 2,591,119 - -0.5 2,591,127 - +0.3 2,591,178 - ypfH b2473 0.12 -0.9 2,591,178 - ypfH b2473 0.12 -0.5 2,591,358 - ypfH b2473 0.38 -0.1 2,591,372 + ypfH b2473 0.40 +0.0 2,591,372 + ypfH b2473 0.40 -0.2 2,591,380 - ypfH b2473 0.41 +0.2 2,591,489 + ypfH b2473 0.57 +0.4 2,591,494 + ypfH b2473 0.57 -1.1 2,591,584 - ypfH b2473 0.70 -0.9 2,591,584 - ypfH b2473 0.70 -1.6 2,591,680 - ypfH b2473 0.84 +0.2 2,591,720 - ypfH b2473 0.90 +0.7 2,591,720 - ypfH b2473 0.90 +0.1 2,591,752 + -0.5 2,591,912 - -0.4 2,591,978 - +0.6 2,591,978 - +1.5 2,591,999 - +0.3 2,592,010 - +0.7 2,592,105 - ypfI b2474 0.12 +0.4 2,592,105 - ypfI b2474 0.12 +0.4 2,592,141 + ypfI b2474 0.14 -0.0 2,592,292 - ypfI b2474 0.21 -0.1 2,592,292 - ypfI b2474 0.21 +0.6 2,592,385 + ypfI b2474 0.26 +0.3 2,592,385 + ypfI b2474 0.26 +0.2 2,592,426 + ypfI b2474 0.28 +2.1 2,592,448 + ypfI b2474 0.29 +0.0 2,592,471 - ypfI b2474 0.30 -0.1 2,592,531 - ypfI b2474 0.33 -0.7 2,592,531 - ypfI b2474 0.33 -0.0 2,592,535 - ypfI b2474 0.33 +0.5 2,592,557 + ypfI b2474 0.34 -0.6 2,592,576 - ypfI b2474 0.35 +0.5 2,592,576 - ypfI b2474 0.35 +1.0 2,592,581 - ypfI b2474 0.35 +0.4 2,592,581 - ypfI b2474 0.35 +0.7 2,592,751 - ypfI b2474 0.44 +1.3 2,592,784 - ypfI b2474 0.46 +0.4 2,592,942 + ypfI b2474 0.53 +0.3 2,592,942 + ypfI b2474 0.53 +1.0 2,592,942 + ypfI b2474 0.53 +1.4 2,593,020 - ypfI b2474 0.57 +0.2 2,593,303 + ypfI b2474 0.71 +0.2 2,593,380 - ypfI b2474 0.75 +1.0 2,593,380 - ypfI b2474 0.75 +0.2 2,593,380 - ypfI b2474 0.75 +1.5 2,593,394 - ypfI b2474 0.76 -0.4 2,593,424 - ypfI b2474 0.77 +1.1 2,593,424 - ypfI b2474 0.77 +0.0 2,593,509 - ypfI b2474 0.81 -0.4 2,593,509 - ypfI b2474 0.81 +1.1 2,593,767 + +0.4 2,593,775 - +0.2 2,593,775 - +0.1 2,593,960 - -0.2 2,593,973 + -0.1 2,594,113 - ypfJ b2475 0.25 +1.3 2,594,113 - ypfJ b2475 0.25 -0.1 2,594,224 + ypfJ b2475 0.38 +0.7 2,594,224 + ypfJ b2475 0.38 -0.1 2,594,282 - ypfJ b2475 0.45 +1.2 2,594,282 - ypfJ b2475 0.45 -0.1 2,594,331 - ypfJ b2475 0.50 +0.2 2,594,338 + ypfJ b2475 0.51 -0.1 2,594,375 + ypfJ b2475 0.55 -0.1 2,594,385 - ypfJ b2475 0.57 -0.5 2,594,430 - ypfJ b2475 0.62 -0.5 2,594,449 + ypfJ b2475 0.64 +0.7 2,594,449 + ypfJ b2475 0.64 -0.2 2,594,449 + ypfJ b2475 0.64 +0.0 2,594,449 - ypfJ b2475 0.64 -0.3 2,594,540 + ypfJ b2475 0.75 -0.4 2,594,573 + ypfJ b2475 0.78 +0.3 2,594,608 - ypfJ b2475 0.82 +0.6 2,594,720 + +1.1 2,594,872 - +0.4
Or see this region's nucleotide sequence