Strain Fitness in Escherichia coli BW25113 around b2150

Experiment: D-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmglA and mglB are separated by 60 nucleotidesmglB and galS are separated by 279 nucleotides b2149: mglA - fused methyl-galactoside transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 2,235,791 to 2,237,311 mglA b2150: mglB - methyl-galactoside transporter subunit (NCBI), at 2,237,372 to 2,238,370 mglB b2151: galS - DNA-binding transcriptional repressor (NCBI), at 2,238,650 to 2,239,690 galS Position (kb) 2237 2238 2239Strain fitness (log2 ratio) -2 -1 0 1 2at 2236.387 kb on - strand, within mglAat 2236.421 kb on - strand, within mglAat 2236.446 kb on + strand, within mglAat 2236.466 kb on - strand, within mglAat 2236.478 kb on - strand, within mglAat 2236.510 kb on - strand, within mglAat 2236.510 kb on - strand, within mglAat 2236.559 kb on + strand, within mglAat 2236.579 kb on - strand, within mglAat 2236.579 kb on - strand, within mglAat 2236.626 kb on - strand, within mglAat 2236.697 kb on + strand, within mglAat 2236.697 kb on + strand, within mglAat 2236.774 kb on + strand, within mglAat 2236.895 kb on + strand, within mglAat 2236.901 kb on - strand, within mglAat 2236.943 kb on - strand, within mglAat 2236.947 kb on + strand, within mglAat 2236.959 kb on + strand, within mglAat 2236.962 kb on - strand, within mglAat 2236.962 kb on - strand, within mglAat 2236.988 kb on + strand, within mglAat 2236.988 kb on - strand, within mglAat 2237.034 kb on + strand, within mglAat 2237.079 kb on - strand, within mglAat 2237.084 kb on + strand, within mglAat 2237.193 kb on + strandat 2237.238 kb on - strandat 2237.249 kb on - strandat 2237.267 kb on - strandat 2237.291 kb on - strandat 2237.296 kb on - strandat 2237.296 kb on - strandat 2237.336 kb on - strandat 2237.356 kb on - strandat 2237.356 kb on - strandat 2237.356 kb on - strandat 2237.356 kb on - strandat 2237.407 kb on - strandat 2237.407 kb on - strandat 2237.422 kb on - strandat 2237.422 kb on - strandat 2237.430 kb on - strandat 2237.530 kb on - strand, within mglBat 2237.897 kb on - strand, within mglBat 2237.901 kb on + strand, within mglBat 2237.927 kb on - strand, within mglBat 2237.989 kb on - strand, within mglBat 2238.223 kb on - strand, within mglBat 2238.269 kb on + strand, within mglBat 2238.309 kb on + strandat 2238.309 kb on + strandat 2238.488 kb on + strandat 2238.488 kb on + strandat 2238.602 kb on + strandat 2238.602 kb on + strandat 2238.647 kb on + strandat 2238.762 kb on - strand, within galSat 2238.762 kb on - strand, within galSat 2238.839 kb on - strand, within galSat 2238.857 kb on - strand, within galSat 2239.012 kb on + strand, within galSat 2239.042 kb on - strand, within galSat 2239.066 kb on + strand, within galSat 2239.098 kb on - strand, within galSat 2239.098 kb on - strand, within galSat 2239.112 kb on - strand, within galSat 2239.267 kb on - strand, within galS

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Lactate (C)
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2,236,387 - mglA b2149 0.39 -1.8
2,236,421 - mglA b2149 0.41 +0.1
2,236,446 + mglA b2149 0.43 -1.2
2,236,466 - mglA b2149 0.44 +0.8
2,236,478 - mglA b2149 0.45 +0.4
2,236,510 - mglA b2149 0.47 -2.0
2,236,510 - mglA b2149 0.47 -1.5
2,236,559 + mglA b2149 0.50 -0.1
2,236,579 - mglA b2149 0.52 +0.7
2,236,579 - mglA b2149 0.52 -0.4
2,236,626 - mglA b2149 0.55 +0.4
2,236,697 + mglA b2149 0.60 +0.1
2,236,697 + mglA b2149 0.60 +0.8
2,236,774 + mglA b2149 0.65 -0.3
2,236,895 + mglA b2149 0.73 -0.4
2,236,901 - mglA b2149 0.73 +0.2
2,236,943 - mglA b2149 0.76 +0.3
2,236,947 + mglA b2149 0.76 -1.8
2,236,959 + mglA b2149 0.77 -0.2
2,236,962 - mglA b2149 0.77 +0.4
2,236,962 - mglA b2149 0.77 +1.5
2,236,988 + mglA b2149 0.79 -0.7
2,236,988 - mglA b2149 0.79 -1.5
2,237,034 + mglA b2149 0.82 -0.9
2,237,079 - mglA b2149 0.85 +0.3
2,237,084 + mglA b2149 0.85 -0.3
2,237,193 + -1.1
2,237,238 - -1.2
2,237,249 - +0.7
2,237,267 - -0.1
2,237,291 - -0.9
2,237,296 - +1.1
2,237,296 - -0.4
2,237,336 - +0.2
2,237,356 - -0.1
2,237,356 - -0.5
2,237,356 - +0.3
2,237,356 - -0.5
2,237,407 - +0.3
2,237,407 - -0.2
2,237,422 - -1.6
2,237,422 - +1.7
2,237,430 - -0.2
2,237,530 - mglB b2150 0.16 -0.2
2,237,897 - mglB b2150 0.53 -0.8
2,237,901 + mglB b2150 0.53 -0.3
2,237,927 - mglB b2150 0.56 -0.4
2,237,989 - mglB b2150 0.62 -0.8
2,238,223 - mglB b2150 0.85 -1.0
2,238,269 + mglB b2150 0.90 -0.1
2,238,309 + +0.6
2,238,309 + +0.3
2,238,488 + +0.4
2,238,488 + +0.2
2,238,602 + +0.1
2,238,602 + +2.4
2,238,647 + +0.1
2,238,762 - galS b2151 0.11 +0.4
2,238,762 - galS b2151 0.11 +1.1
2,238,839 - galS b2151 0.18 +0.6
2,238,857 - galS b2151 0.20 +0.9
2,239,012 + galS b2151 0.35 +0.8
2,239,042 - galS b2151 0.38 +1.2
2,239,066 + galS b2151 0.40 -1.2
2,239,098 - galS b2151 0.43 +0.1
2,239,098 - galS b2151 0.43 +0.7
2,239,112 - galS b2151 0.44 -0.0
2,239,267 - galS b2151 0.59 -1.5

Or see this region's nucleotide sequence