Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydjQ and b1742 overlap by 41 nucleotides b1742 and spy are separated by 202 nucleotides spy and astE are separated by 329 nucleotides
b1741: ydjQ - endonuclease of nucleotide excision repair (NCBI), at 1,821,539 to 1,822,426
ydjQ
b1742: b1742 - orf, hypothetical protein (VIMSS), at 1,822,386 to 1,822,961
b1742
b1743: spy - envelope stress induced periplasmic protein (NCBI), at 1,823,164 to 1,823,649
spy
b1744: astE - succinylglutamate desuccinylase (NCBI), at 1,823,979 to 1,824,947
astE
Position (kb)
1823
1824 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1822.304 kb on + strand, within ydjQ at 1822.740 kb on - strand, within b1742 at 1822.740 kb on - strand, within b1742 at 1822.963 kb on - strand at 1823.108 kb on + strand at 1823.191 kb on - strand at 1823.293 kb on - strand, within spy at 1823.519 kb on + strand, within spy at 1823.549 kb on + strand, within spy at 1823.549 kb on + strand, within spy at 1823.559 kb on + strand, within spy at 1823.587 kb on + strand, within spy at 1823.595 kb on - strand, within spy at 1823.595 kb on - strand, within spy at 1823.613 kb on + strand at 1823.621 kb on - strand at 1823.629 kb on - strand at 1823.629 kb on - strand at 1823.683 kb on + strand at 1823.686 kb on + strand at 1823.703 kb on + strand at 1823.711 kb on - strand at 1823.729 kb on - strand at 1824.017 kb on - strand at 1824.017 kb on - strand at 1824.032 kb on + strand at 1824.047 kb on - strand at 1824.047 kb on - strand at 1824.052 kb on - strand at 1824.053 kb on - strand at 1824.059 kb on - strand at 1824.059 kb on - strand at 1824.087 kb on + strand, within astE at 1824.090 kb on - strand, within astE at 1824.090 kb on - strand, within astE at 1824.095 kb on - strand, within astE at 1824.095 kb on - strand, within astE at 1824.095 kb on - strand, within astE at 1824.095 kb on - strand, within astE at 1824.330 kb on + strand, within astE at 1824.333 kb on - strand, within astE at 1824.380 kb on + strand, within astE at 1824.397 kb on + strand, within astE at 1824.512 kb on + strand, within astE
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 1,822,304 + ydjQ b1741 0.86 -0.3 1,822,740 - b1742 b1742 0.61 +0.5 1,822,740 - b1742 b1742 0.61 +0.2 1,822,963 - -1.1 1,823,108 + +0.3 1,823,191 - -1.3 1,823,293 - spy b1743 0.27 +0.2 1,823,519 + spy b1743 0.73 -0.8 1,823,549 + spy b1743 0.79 +0.3 1,823,549 + spy b1743 0.79 +0.4 1,823,559 + spy b1743 0.81 +2.7 1,823,587 + spy b1743 0.87 -0.1 1,823,595 - spy b1743 0.89 +0.3 1,823,595 - spy b1743 0.89 -0.3 1,823,613 + -0.3 1,823,621 - -2.7 1,823,629 - -0.5 1,823,629 - -1.8 1,823,683 + -0.0 1,823,686 + +0.2 1,823,703 + +0.5 1,823,711 - -0.5 1,823,729 - -0.5 1,824,017 - +0.6 1,824,017 - -0.6 1,824,032 + -1.7 1,824,047 - -0.6 1,824,047 - -0.2 1,824,052 - -0.1 1,824,053 - -0.2 1,824,059 - -1.3 1,824,059 - +0.1 1,824,087 + astE b1744 0.11 +0.1 1,824,090 - astE b1744 0.11 -1.3 1,824,090 - astE b1744 0.11 -1.1 1,824,095 - astE b1744 0.12 -0.3 1,824,095 - astE b1744 0.12 +0.1 1,824,095 - astE b1744 0.12 -0.7 1,824,095 - astE b1744 0.12 +0.4 1,824,330 + astE b1744 0.36 +0.7 1,824,333 - astE b1744 0.37 -0.6 1,824,380 + astE b1744 0.41 -1.0 1,824,397 + astE b1744 0.43 +0.8 1,824,512 + astE b1744 0.55 +0.2
Or see this region's nucleotide sequence