Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aroP and pdhR are separated by 540 nucleotides pdhR and aceE are separated by 160 nucleotides
b0112: aroP - aromatic amino acid transporter (NCBI), at 120,178 to 121,551
aroP
b0113: pdhR - transcriptional regulator of pyruvate dehydrogenase complex (NCBI), at 122,092 to 122,856
pdhR
b0114: aceE - pyruvate dehydrogenase subunit E1 (NCBI), at 123,017 to 125,680
aceE
Position (kb)
122
123 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 121.095 kb on - strand, within aroP at 121.128 kb on - strand, within aroP at 121.128 kb on - strand, within aroP at 121.146 kb on - strand, within aroP at 121.146 kb on - strand, within aroP at 121.266 kb on - strand, within aroP at 121.266 kb on - strand, within aroP at 121.335 kb on - strand, within aroP at 121.374 kb on - strand, within aroP at 121.374 kb on - strand, within aroP at 121.431 kb on + strand at 121.431 kb on + strand at 121.458 kb on - strand at 121.469 kb on - strand at 121.514 kb on + strand at 121.531 kb on - strand at 121.716 kb on - strand at 121.716 kb on - strand at 121.723 kb on - strand at 121.723 kb on - strand at 121.738 kb on - strand at 122.355 kb on + strand, within pdhR at 122.358 kb on + strand, within pdhR at 122.369 kb on + strand, within pdhR at 122.416 kb on + strand, within pdhR at 122.442 kb on + strand, within pdhR at 122.471 kb on + strand, within pdhR at 122.489 kb on + strand, within pdhR at 122.489 kb on + strand, within pdhR at 122.489 kb on + strand, within pdhR at 122.500 kb on + strand, within pdhR at 122.537 kb on + strand, within pdhR at 122.814 kb on + strand at 123.002 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 121,095 - aroP b0112 0.67 +0.9 121,128 - aroP b0112 0.69 -0.1 121,128 - aroP b0112 0.69 -0.7 121,146 - aroP b0112 0.70 +0.1 121,146 - aroP b0112 0.70 +0.8 121,266 - aroP b0112 0.79 +0.1 121,266 - aroP b0112 0.79 +0.2 121,335 - aroP b0112 0.84 -0.2 121,374 - aroP b0112 0.87 -1.6 121,374 - aroP b0112 0.87 -1.8 121,431 + -1.7 121,431 + +1.5 121,458 - -1.1 121,469 - -1.2 121,514 + -0.5 121,531 - -1.1 121,716 - +0.2 121,716 - +0.5 121,723 - -0.8 121,723 - +0.3 121,738 - +0.4 122,355 + pdhR b0113 0.34 +0.4 122,358 + pdhR b0113 0.35 -3.6 122,369 + pdhR b0113 0.36 -0.9 122,416 + pdhR b0113 0.42 -2.7 122,442 + pdhR b0113 0.46 -3.5 122,471 + pdhR b0113 0.50 -4.3 122,489 + pdhR b0113 0.52 -0.1 122,489 + pdhR b0113 0.52 -2.5 122,489 + pdhR b0113 0.52 +0.2 122,500 + pdhR b0113 0.53 -0.7 122,537 + pdhR b0113 0.58 -1.9 122,814 + -2.5 123,002 + -2.1
Or see this region's nucleotide sequence