Experiment: KB with Nalidixic 0.775 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0289 and Psyr_0290 are separated by 122 nucleotides Psyr_0290 and Psyr_0291 are separated by 186 nucleotides Psyr_0291 and Psyr_0292 are separated by 231 nucleotides
Psyr_0289: Psyr_0289 - Radical SAM, at 307,483 to 308,541
_0289
Psyr_0290: Psyr_0290 - ThiJ/PfpI, at 308,664 to 309,332
_0290
Psyr_0291: Psyr_0291 - DedA, at 309,519 to 310,181
_0291
Psyr_0292: Psyr_0292 - porphobilinogen synthase, at 310,413 to 311,423
_0292
Position (kb) 309
310
311 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 308.550 kb on - strand at 308.555 kb on + strand at 308.581 kb on - strand at 308.582 kb on + strand at 308.582 kb on + strand at 308.582 kb on + strand at 308.582 kb on + strand at 308.583 kb on - strand at 308.583 kb on - strand at 308.583 kb on - strand at 308.583 kb on - strand at 308.755 kb on + strand, within Psyr_0290 at 308.755 kb on + strand, within Psyr_0290 at 308.755 kb on + strand, within Psyr_0290 at 308.755 kb on + strand, within Psyr_0290 at 308.755 kb on + strand, within Psyr_0290 at 308.755 kb on + strand, within Psyr_0290 at 308.755 kb on + strand, within Psyr_0290 at 308.756 kb on - strand, within Psyr_0290 at 308.756 kb on - strand, within Psyr_0290 at 308.756 kb on - strand, within Psyr_0290 at 308.756 kb on - strand, within Psyr_0290 at 308.756 kb on - strand, within Psyr_0290 at 308.756 kb on - strand, within Psyr_0290 at 308.756 kb on - strand, within Psyr_0290 at 308.756 kb on - strand, within Psyr_0290 at 308.896 kb on + strand, within Psyr_0290 at 308.897 kb on - strand, within Psyr_0290 at 308.897 kb on - strand, within Psyr_0290 at 308.897 kb on - strand, within Psyr_0290 at 309.313 kb on - strand at 309.375 kb on - strand at 309.400 kb on - strand at 309.681 kb on + strand, within Psyr_0291 at 309.681 kb on + strand, within Psyr_0291 at 309.709 kb on + strand, within Psyr_0291 at 309.709 kb on + strand, within Psyr_0291 at 309.710 kb on - strand, within Psyr_0291 at 309.818 kb on - strand, within Psyr_0291 at 309.878 kb on - strand, within Psyr_0291 at 310.006 kb on + strand, within Psyr_0291 at 310.006 kb on + strand, within Psyr_0291 at 310.006 kb on + strand, within Psyr_0291 at 310.006 kb on + strand, within Psyr_0291 at 310.007 kb on - strand, within Psyr_0291 at 310.007 kb on - strand, within Psyr_0291 at 310.007 kb on - strand, within Psyr_0291 at 310.027 kb on + strand, within Psyr_0291 at 310.072 kb on + strand, within Psyr_0291 at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.261 kb on + strand at 310.356 kb on + strand at 310.367 kb on + strand at 310.367 kb on + strand at 310.367 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nalidixic 0.775 ug/ml remove 308,550 - +0.4 308,555 + +0.1 308,581 - -0.9 308,582 + -1.3 308,582 + -0.1 308,582 + -0.4 308,582 + +0.6 308,583 - -0.2 308,583 - +1.1 308,583 - -0.4 308,583 - -3.0 308,755 + Psyr_0290 0.14 -0.9 308,755 + Psyr_0290 0.14 +1.1 308,755 + Psyr_0290 0.14 +0.6 308,755 + Psyr_0290 0.14 -2.0 308,755 + Psyr_0290 0.14 +0.2 308,755 + Psyr_0290 0.14 +0.7 308,755 + Psyr_0290 0.14 +0.7 308,756 - Psyr_0290 0.14 -1.6 308,756 - Psyr_0290 0.14 -0.5 308,756 - Psyr_0290 0.14 +0.5 308,756 - Psyr_0290 0.14 -1.3 308,756 - Psyr_0290 0.14 +0.1 308,756 - Psyr_0290 0.14 +0.5 308,756 - Psyr_0290 0.14 -1.2 308,756 - Psyr_0290 0.14 +0.9 308,896 + Psyr_0290 0.35 +0.2 308,897 - Psyr_0290 0.35 -0.4 308,897 - Psyr_0290 0.35 -0.5 308,897 - Psyr_0290 0.35 -0.2 309,313 - +0.1 309,375 - -0.4 309,400 - -0.3 309,681 + Psyr_0291 0.24 +1.8 309,681 + Psyr_0291 0.24 -0.4 309,709 + Psyr_0291 0.29 -0.6 309,709 + Psyr_0291 0.29 -1.6 309,710 - Psyr_0291 0.29 -0.2 309,818 - Psyr_0291 0.45 +1.2 309,878 - Psyr_0291 0.54 +1.2 310,006 + Psyr_0291 0.73 -2.5 310,006 + Psyr_0291 0.73 -0.1 310,006 + Psyr_0291 0.73 +0.3 310,006 + Psyr_0291 0.73 +0.8 310,007 - Psyr_0291 0.74 +0.4 310,007 - Psyr_0291 0.74 +1.2 310,007 - Psyr_0291 0.74 -0.4 310,027 + Psyr_0291 0.77 +1.0 310,072 + Psyr_0291 0.83 -0.3 310,246 + -0.2 310,246 + -0.3 310,246 + +0.6 310,246 + -0.6 310,246 + +0.3 310,246 + +0.5 310,246 + +0.6 310,246 + -0.6 310,247 - +0.3 310,247 - +0.4 310,247 - +0.7 310,247 - -0.4 310,247 - +0.5 310,247 - +0.4 310,247 - -0.3 310,247 - +1.2 310,247 - -1.2 310,247 - -0.7 310,247 - -0.7 310,247 - +0.8 310,247 - +1.0 310,247 - -0.4 310,247 - +0.2 310,247 - +0.6 310,261 + -0.4 310,356 + -0.1 310,367 + +1.2 310,367 + -0.2 310,367 + -0.8 310,369 + +0.3 310,369 + -1.0 310,369 + -0.8 310,369 + +0.3 310,369 + -0.1 310,369 + +0.2 310,369 + -0.1 310,369 + -0.8
Or see this region's nucleotide sequence