Strain Fitness in Pseudomonas fluorescens GW456-L13 around PfGW456L13_859

Experiment: Putrescine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPfGW456L13_858 and PfGW456L13_859 overlap by 4 nucleotidesPfGW456L13_859 and PfGW456L13_860 are separated by 263 nucleotidesPfGW456L13_860 and PfGW456L13_861 are separated by 177 nucleotides PfGW456L13_858 - Uroporphyrinogen-III synthase (EC 4.2.1.75), at 964,293 to 965,456 _858 PfGW456L13_859 - Uncharacterized protein EC-HemY, likely associated with heme metabolism based on gene clustering with hemC, hemD in Proteobacteria (unrelated to HemY-type PPO in GramPositives), at 965,453 to 966,691 _859 PfGW456L13_860 - Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation, at 966,955 to 967,506 _860 PfGW456L13_861 - Regulator of sigma D, at 967,684 to 968,142 _861 Position (kb) 965 966 967Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 964.499 kb on + strand, within PfGW456L13_858at 964.642 kb on + strand, within PfGW456L13_858at 964.643 kb on - strand, within PfGW456L13_858at 964.643 kb on - strand, within PfGW456L13_858at 964.813 kb on + strand, within PfGW456L13_858at 964.977 kb on + strand, within PfGW456L13_858at 964.977 kb on + strand, within PfGW456L13_858at 964.977 kb on + strand, within PfGW456L13_858at 964.978 kb on - strand, within PfGW456L13_858at 964.978 kb on - strand, within PfGW456L13_858at 964.978 kb on - strand, within PfGW456L13_858at 965.068 kb on - strand, within PfGW456L13_858at 965.206 kb on - strand, within PfGW456L13_858at 965.465 kb on + strandat 965.465 kb on + strandat 965.465 kb on + strandat 965.466 kb on - strandat 965.466 kb on - strandat 965.466 kb on - strandat 965.549 kb on + strandat 965.550 kb on - strandat 965.584 kb on + strand, within PfGW456L13_859at 965.585 kb on - strand, within PfGW456L13_859at 965.813 kb on + strand, within PfGW456L13_859at 965.814 kb on - strand, within PfGW456L13_859at 965.814 kb on - strand, within PfGW456L13_859at 965.816 kb on + strand, within PfGW456L13_859at 965.817 kb on - strand, within PfGW456L13_859at 965.817 kb on - strand, within PfGW456L13_859at 966.275 kb on + strand, within PfGW456L13_859at 966.276 kb on - strand, within PfGW456L13_859at 966.276 kb on - strand, within PfGW456L13_859at 966.350 kb on + strand, within PfGW456L13_859at 966.350 kb on + strand, within PfGW456L13_859at 966.374 kb on + strand, within PfGW456L13_859at 966.374 kb on + strand, within PfGW456L13_859at 966.480 kb on + strand, within PfGW456L13_859at 966.480 kb on + strand, within PfGW456L13_859at 966.480 kb on + strand, within PfGW456L13_859at 966.480 kb on + strand, within PfGW456L13_859at 966.481 kb on - strand, within PfGW456L13_859at 966.481 kb on - strand, within PfGW456L13_859at 966.518 kb on + strand, within PfGW456L13_859at 966.518 kb on + strand, within PfGW456L13_859at 966.540 kb on + strand, within PfGW456L13_859at 966.773 kb on + strandat 966.773 kb on + strandat 966.774 kb on - strandat 966.774 kb on - strandat 966.774 kb on - strandat 966.774 kb on - strandat 966.774 kb on - strandat 966.816 kb on - strandat 966.825 kb on + strandat 966.825 kb on + strandat 966.825 kb on + strandat 966.825 kb on + strandat 966.826 kb on - strandat 966.826 kb on - strandat 966.826 kb on - strandat 966.826 kb on - strandat 966.826 kb on - strandat 966.826 kb on - strandat 966.832 kb on + strandat 966.833 kb on - strandat 966.860 kb on + strandat 966.860 kb on + strandat 966.861 kb on - strandat 966.919 kb on - strandat 966.923 kb on + strandat 966.937 kb on + strandat 966.938 kb on - strandat 966.938 kb on - strandat 967.027 kb on + strand, within PfGW456L13_860at 967.027 kb on + strand, within PfGW456L13_860at 967.028 kb on - strand, within PfGW456L13_860at 967.028 kb on - strand, within PfGW456L13_860at 967.028 kb on - strand, within PfGW456L13_860at 967.030 kb on + strand, within PfGW456L13_860at 967.030 kb on + strand, within PfGW456L13_860at 967.031 kb on - strand, within PfGW456L13_860at 967.039 kb on + strand, within PfGW456L13_860at 967.039 kb on + strand, within PfGW456L13_860at 967.039 kb on + strand, within PfGW456L13_860at 967.039 kb on + strand, within PfGW456L13_860at 967.040 kb on - strand, within PfGW456L13_860at 967.159 kb on + strand, within PfGW456L13_860at 967.159 kb on + strand, within PfGW456L13_860at 967.159 kb on + strand, within PfGW456L13_860at 967.159 kb on + strand, within PfGW456L13_860at 967.307 kb on + strand, within PfGW456L13_860at 967.308 kb on - strand, within PfGW456L13_860at 967.423 kb on + strand, within PfGW456L13_860at 967.505 kb on - strandat 967.514 kb on + strandat 967.514 kb on + strandat 967.514 kb on + strandat 967.515 kb on - strandat 967.515 kb on - strandat 967.515 kb on - strandat 967.515 kb on - strandat 967.515 kb on - strandat 967.515 kb on - strandat 967.515 kb on - strandat 967.524 kb on - strandat 967.524 kb on - strandat 967.534 kb on + strandat 967.534 kb on + strandat 967.535 kb on - strandat 967.561 kb on + strandat 967.589 kb on + strandat 967.589 kb on + strandat 967.589 kb on + strandat 967.589 kb on + strandat 967.589 kb on + strandat 967.589 kb on + strandat 967.589 kb on + strandat 967.589 kb on + strandat 967.589 kb on + strandat 967.589 kb on + strandat 967.590 kb on - strandat 967.590 kb on - strandat 967.590 kb on - strandat 967.590 kb on - strandat 967.590 kb on - strandat 967.590 kb on - strandat 967.590 kb on - strandat 967.630 kb on + strandat 967.630 kb on + strandat 967.630 kb on + strandat 967.630 kb on + strandat 967.630 kb on + strandat 967.631 kb on - strandat 967.631 kb on - strandat 967.631 kb on - strandat 967.631 kb on - strandat 967.649 kb on + strandat 967.650 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Putrescine (C)
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964,499 + PfGW456L13_858 0.18 +0.0
964,642 + PfGW456L13_858 0.30 -1.0
964,643 - PfGW456L13_858 0.30 +1.8
964,643 - PfGW456L13_858 0.30 +0.5
964,813 + PfGW456L13_858 0.45 -0.3
964,977 + PfGW456L13_858 0.59 +1.4
964,977 + PfGW456L13_858 0.59 +0.4
964,977 + PfGW456L13_858 0.59 -1.2
964,978 - PfGW456L13_858 0.59 +0.8
964,978 - PfGW456L13_858 0.59 -0.6
964,978 - PfGW456L13_858 0.59 -0.5
965,068 - PfGW456L13_858 0.67 -0.7
965,206 - PfGW456L13_858 0.78 -0.5
965,465 + -0.8
965,465 + +0.8
965,465 + -0.3
965,466 - +0.1
965,466 - -0.3
965,466 - +1.0
965,549 + -0.2
965,550 - +0.8
965,584 + PfGW456L13_859 0.11 -1.5
965,585 - PfGW456L13_859 0.11 -0.0
965,813 + PfGW456L13_859 0.29 +0.5
965,814 - PfGW456L13_859 0.29 +0.4
965,814 - PfGW456L13_859 0.29 +1.0
965,816 + PfGW456L13_859 0.29 -1.0
965,817 - PfGW456L13_859 0.29 +1.5
965,817 - PfGW456L13_859 0.29 +1.0
966,275 + PfGW456L13_859 0.66 -1.2
966,276 - PfGW456L13_859 0.66 +0.4
966,276 - PfGW456L13_859 0.66 -0.9
966,350 + PfGW456L13_859 0.72 +0.2
966,350 + PfGW456L13_859 0.72 +1.8
966,374 + PfGW456L13_859 0.74 -0.2
966,374 + PfGW456L13_859 0.74 -0.8
966,480 + PfGW456L13_859 0.83 +1.1
966,480 + PfGW456L13_859 0.83 -1.0
966,480 + PfGW456L13_859 0.83 -0.7
966,480 + PfGW456L13_859 0.83 -1.5
966,481 - PfGW456L13_859 0.83 +0.0
966,481 - PfGW456L13_859 0.83 +1.0
966,518 + PfGW456L13_859 0.86 +0.0
966,518 + PfGW456L13_859 0.86 -0.5
966,540 + PfGW456L13_859 0.88 +1.2
966,773 + +0.8
966,773 + -0.5
966,774 - -0.2
966,774 - -0.8
966,774 - -1.5
966,774 - -0.6
966,774 - +0.4
966,816 - +1.3
966,825 + -0.2
966,825 + +0.5
966,825 + +0.7
966,825 + +0.8
966,826 - +1.0
966,826 - +1.4
966,826 - +0.4
966,826 - -1.0
966,826 - -0.1
966,826 - -0.2
966,832 + -0.6
966,833 - -0.3
966,860 + -0.6
966,860 + +1.4
966,861 - +3.4
966,919 - +0.9
966,923 + -0.7
966,937 + +1.7
966,938 - +1.0
966,938 - -0.9
967,027 + PfGW456L13_860 0.13 -0.7
967,027 + PfGW456L13_860 0.13 -2.2
967,028 - PfGW456L13_860 0.13 -0.1
967,028 - PfGW456L13_860 0.13 -0.8
967,028 - PfGW456L13_860 0.13 +0.4
967,030 + PfGW456L13_860 0.14 +1.1
967,030 + PfGW456L13_860 0.14 -1.1
967,031 - PfGW456L13_860 0.14 +0.4
967,039 + PfGW456L13_860 0.15 +0.4
967,039 + PfGW456L13_860 0.15 -0.4
967,039 + PfGW456L13_860 0.15 +0.5
967,039 + PfGW456L13_860 0.15 -1.1
967,040 - PfGW456L13_860 0.15 +0.1
967,159 + PfGW456L13_860 0.37 +0.5
967,159 + PfGW456L13_860 0.37 +0.4
967,159 + PfGW456L13_860 0.37 +2.2
967,159 + PfGW456L13_860 0.37 -1.3
967,307 + PfGW456L13_860 0.64 -1.9
967,308 - PfGW456L13_860 0.64 +1.2
967,423 + PfGW456L13_860 0.85 +0.8
967,505 - +0.4
967,514 + +1.4
967,514 + -1.0
967,514 + -1.6
967,515 - -0.7
967,515 - -0.2
967,515 - -0.5
967,515 - +0.3
967,515 - +1.0
967,515 - -1.2
967,515 - +0.5
967,524 - +0.4
967,524 - +1.5
967,534 + -0.4
967,534 + -1.1
967,535 - +1.7
967,561 + +0.2
967,589 + +1.0
967,589 + +1.4
967,589 + +0.2
967,589 + -1.1
967,589 + -3.0
967,589 + -0.4
967,589 + +0.8
967,589 + -1.2
967,589 + +0.4
967,589 + +1.0
967,590 - +0.2
967,590 - -0.1
967,590 - +0.3
967,590 - +0.0
967,590 - +0.7
967,590 - -1.9
967,590 - -1.1
967,630 + +0.4
967,630 + +0.5
967,630 + -0.3
967,630 + -0.2
967,630 + -0.0
967,631 - -0.5
967,631 - +0.5
967,631 - +1.6
967,631 - -0.5
967,649 + +0.6
967,650 - +0.6

Or see this region's nucleotide sequence