Experiment: MS_media no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0189 and EX28DRAFT_0190 are separated by 10 nucleotides EX28DRAFT_0190 and EX28DRAFT_0191 are separated by 556 nucleotides
EX28DRAFT_0189: EX28DRAFT_0189 - succinyldiaminopimelate desuccinylase (EC 3.5.1.18), at 138,284 to 139,411
_0189
EX28DRAFT_0190: EX28DRAFT_0190 - transcriptional regulator, Spx/MgsR family, at 139,422 to 139,772
_0190
EX28DRAFT_0191: EX28DRAFT_0191 - The (Largely Gram-negative Bacterial) Hydrophobe/Amphiphile Efflux-1 (HAE1) Family, at 140,329 to 143,442
_0191
Position (kb)
139
140 Strain fitness (log2 ratio)
-2
-1
0
1 at 139.469 kb on + strand, within EX28DRAFT_0190 at 139.470 kb on - strand, within EX28DRAFT_0190 at 139.800 kb on + strand at 139.801 kb on - strand at 139.824 kb on - strand at 139.842 kb on + strand at 139.869 kb on - strand at 139.929 kb on - strand at 140.047 kb on + strand at 140.101 kb on + strand at 140.140 kb on + strand at 140.143 kb on + strand at 140.146 kb on + strand at 140.266 kb on - strand at 140.275 kb on + strand at 140.394 kb on - strand at 140.536 kb on + strand at 140.537 kb on - strand at 140.537 kb on - strand at 140.672 kb on + strand, within EX28DRAFT_0191 at 140.672 kb on + strand, within EX28DRAFT_0191 at 140.673 kb on - strand, within EX28DRAFT_0191
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media no stress control remove 139,469 + EX28DRAFT_0190 0.13 -2.5 139,470 - EX28DRAFT_0190 0.14 +0.3 139,800 + +0.3 139,801 - -0.0 139,824 - +0.3 139,842 + -0.8 139,869 - -0.2 139,929 - -0.7 140,047 + -1.0 140,101 + +0.5 140,140 + +0.1 140,143 + +0.3 140,146 + +0.2 140,266 - +0.1 140,275 + +0.1 140,394 - +1.2 140,536 + -0.4 140,537 - -0.3 140,537 - -0.1 140,672 + EX28DRAFT_0191 0.11 +1.2 140,672 + EX28DRAFT_0191 0.11 -0.1 140,673 - EX28DRAFT_0191 0.11 +1.4
Or see this region's nucleotide sequence