Strain Fitness in Escherichia coli BL21 around ECD_00228

Experiment: NophageControl

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00226 and ECD_00227 are separated by 51 nucleotidesECD_00227 and ECD_00228 are separated by 2 nucleotidesECD_00228 and ECD_00229 are separated by 9 nucleotidesECD_00229 and ECD_00230 are separated by 233 nucleotidesECD_00230 and ECD_00231 overlap by 32 nucleotides ECD_00226: ECD_00226 - DNA polymerase IV, at 255,464 to 256,519 _00226 ECD_00227: ECD_00227 - antitoxin of the YafO-YafN toxin-antitoxin system, at 256,571 to 256,864 _00227 ECD_00228: ECD_00228 - mRNA interferase toxin of the YafO-YafN toxin-antitoxin system, at 256,867 to 257,265 _00228 ECD_00229: ECD_00229 - GNAT family putative N-acetyltransferase, at 257,275 to 257,727 _00229 ECD_00230: ECD_00230 - hypothetical protein, at 257,961 to 258,227 _00230 ECD_00231: ECD_00231 - peptide chain release factor 2, at 258,196 to 258,696 _00231 Position (kb) 256 257 258Strain fitness (log2 ratio) -1 0 1at 255.990 kb on + strand, within ECD_00226at 256.071 kb on + strand, within ECD_00226at 256.138 kb on + strand, within ECD_00226at 256.306 kb on - strand, within ECD_00226at 256.530 kb on + strandat 256.976 kb on + strand, within ECD_00228at 256.977 kb on - strand, within ECD_00228at 256.977 kb on - strand, within ECD_00228at 257.127 kb on - strand, within ECD_00228at 257.129 kb on - strand, within ECD_00228at 257.516 kb on + strand, within ECD_00229at 257.958 kb on + strandat 257.970 kb on + strandat 258.061 kb on + strand, within ECD_00230at 258.062 kb on - strand, within ECD_00230

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Per-strain Table

Position Strand Gene LocusTag Fraction NophageControl
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255,990 + ECD_00226 0.50 +0.0
256,071 + ECD_00226 0.57 -0.4
256,138 + ECD_00226 0.64 +0.3
256,306 - ECD_00226 0.80 +1.1
256,530 + +1.6
256,976 + ECD_00228 0.27 +0.8
256,977 - ECD_00228 0.28 +0.2
256,977 - ECD_00228 0.28 -0.1
257,127 - ECD_00228 0.65 -0.2
257,129 - ECD_00228 0.66 +1.0
257,516 + ECD_00229 0.53 +0.1
257,958 + +1.1
257,970 + +0.3
258,061 + ECD_00230 0.37 -0.8
258,062 - ECD_00230 0.38 -1.0

Or see this region's nucleotide sequence