Strain Fitness in Escherichia coli BW25113 around b2700

Experiment: acetate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrecA and ygaD are separated by 79 nucleotidesygaD and mltB are separated by 144 nucleotides b2699: recA - recombinase A (NCBI), at 2,820,730 to 2,821,791 recA b2700: ygaD - competence damage-inducible protein A (NCBI), at 2,821,871 to 2,822,368 ygaD b2701: mltB - membrane-bound lytic murein transglycosylase B (NCBI), at 2,822,513 to 2,823,598 mltB Position (kb) 2821 2822 2823Strain fitness (log2 ratio) -2 -1 0 1at 2820.929 kb on + strand, within recAat 2821.141 kb on - strand, within recAat 2821.166 kb on - strand, within recAat 2821.522 kb on + strand, within recAat 2821.638 kb on + strand, within recAat 2821.701 kb on - strandat 2821.810 kb on - strandat 2821.810 kb on - strandat 2822.013 kb on + strand, within ygaDat 2822.231 kb on - strand, within ygaDat 2822.232 kb on + strand, within ygaDat 2822.333 kb on - strandat 2822.358 kb on + strandat 2822.437 kb on - strandat 2822.488 kb on + strandat 2822.520 kb on + strandat 2822.520 kb on + strandat 2822.545 kb on - strandat 2822.598 kb on - strandat 2822.641 kb on - strand, within mltBat 2822.668 kb on + strand, within mltBat 2822.764 kb on - strand, within mltBat 2822.765 kb on - strand, within mltBat 2822.827 kb on + strand, within mltBat 2822.840 kb on - strand, within mltBat 2822.880 kb on - strand, within mltBat 2822.996 kb on + strand, within mltBat 2823.026 kb on - strand, within mltBat 2823.150 kb on + strand, within mltBat 2823.162 kb on + strand, within mltBat 2823.183 kb on + strand, within mltBat 2823.256 kb on + strand, within mltBat 2823.279 kb on - strand, within mltB

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Per-strain Table

Position Strand Gene LocusTag Fraction acetate (C)
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2,820,929 + recA b2699 0.19 -1.1
2,821,141 - recA b2699 0.39 -0.5
2,821,166 - recA b2699 0.41 +0.4
2,821,522 + recA b2699 0.75 +0.5
2,821,638 + recA b2699 0.85 +1.1
2,821,701 - +0.8
2,821,810 - -1.3
2,821,810 - -1.3
2,822,013 + ygaD b2700 0.29 +0.5
2,822,231 - ygaD b2700 0.72 +0.8
2,822,232 + ygaD b2700 0.72 -2.1
2,822,333 - -1.3
2,822,358 + -0.2
2,822,437 - -2.5
2,822,488 + +0.1
2,822,520 + +0.3
2,822,520 + -0.9
2,822,545 - -2.4
2,822,598 - +0.3
2,822,641 - mltB b2701 0.12 +0.0
2,822,668 + mltB b2701 0.14 +0.4
2,822,764 - mltB b2701 0.23 +0.0
2,822,765 - mltB b2701 0.23 +0.2
2,822,827 + mltB b2701 0.29 +0.7
2,822,840 - mltB b2701 0.30 -0.3
2,822,880 - mltB b2701 0.34 -0.4
2,822,996 + mltB b2701 0.44 -1.6
2,823,026 - mltB b2701 0.47 -0.7
2,823,150 + mltB b2701 0.59 +0.5
2,823,162 + mltB b2701 0.60 -1.9
2,823,183 + mltB b2701 0.62 -1.8
2,823,256 + mltB b2701 0.68 -0.2
2,823,279 - mltB b2701 0.71 -0.7

Or see this region's nucleotide sequence