Strain Fitness in Escherichia coli BW25113 around b1869

Experiment: acetate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyecD and yecE overlap by 4 nucleotidesyecE and yecN are separated by 52 nucleotidesyecN and yecO are separated by 40 nucleotidesyecO and yecP overlap by 4 nucleotides b1867: yecD - orf, hypothetical protein (VIMSS), at 1,948,856 to 1,949,422 yecD b1868: yecE - hypothetical protein (NCBI), at 1,949,419 to 1,950,237 yecE b1869: yecN - orf, hypothetical protein (VIMSS), at 1,950,290 to 1,950,685 yecN b1870: yecO - predicted methyltransferase (NCBI), at 1,950,726 to 1,951,469 yecO b1871: yecP - predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI), at 1,951,466 to 1,952,437 yecP Position (kb) 1950 1951Strain fitness (log2 ratio) -3 -2 -1 0 1at 1949.349 kb on + strand, within yecDat 1949.404 kb on - strandat 1949.404 kb on - strandat 1949.442 kb on - strandat 1949.709 kb on + strand, within yecEat 1949.709 kb on + strand, within yecEat 1949.746 kb on - strand, within yecEat 1949.842 kb on - strand, within yecEat 1949.842 kb on - strand, within yecEat 1949.931 kb on + strand, within yecEat 1950.249 kb on + strandat 1950.310 kb on - strandat 1950.444 kb on + strand, within yecNat 1950.475 kb on + strand, within yecNat 1950.475 kb on + strand, within yecNat 1950.509 kb on + strand, within yecNat 1950.517 kb on - strand, within yecNat 1950.525 kb on + strand, within yecNat 1950.525 kb on + strand, within yecNat 1950.612 kb on + strand, within yecNat 1950.612 kb on + strand, within yecNat 1950.613 kb on - strand, within yecNat 1950.613 kb on - strand, within yecNat 1950.743 kb on + strandat 1951.029 kb on + strand, within yecOat 1951.519 kb on - strandat 1951.579 kb on + strand, within yecP

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Per-strain Table

Position Strand Gene LocusTag Fraction acetate (C)
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1,949,349 + yecD b1867 0.87 +1.4
1,949,404 - +0.5
1,949,404 - -0.1
1,949,442 - -0.6
1,949,709 + yecE b1868 0.35 -0.6
1,949,709 + yecE b1868 0.35 -0.8
1,949,746 - yecE b1868 0.40 +0.5
1,949,842 - yecE b1868 0.52 +0.8
1,949,842 - yecE b1868 0.52 +0.4
1,949,931 + yecE b1868 0.63 -0.5
1,950,249 + -0.2
1,950,310 - +0.2
1,950,444 + yecN b1869 0.39 -2.5
1,950,475 + yecN b1869 0.47 -1.4
1,950,475 + yecN b1869 0.47 -0.9
1,950,509 + yecN b1869 0.55 -1.8
1,950,517 - yecN b1869 0.57 +1.0
1,950,525 + yecN b1869 0.59 +0.4
1,950,525 + yecN b1869 0.59 -0.1
1,950,612 + yecN b1869 0.81 -2.3
1,950,612 + yecN b1869 0.81 -0.7
1,950,613 - yecN b1869 0.82 +0.3
1,950,613 - yecN b1869 0.82 +0.6
1,950,743 + -1.1
1,951,029 + yecO b1870 0.41 -1.8
1,951,519 - -3.2
1,951,579 + yecP b1871 0.12 -1.2

Or see this region's nucleotide sequence