Experiment: acetate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tus and fumC overlap by 4 nucleotides fumC and fumA are separated by 142 nucleotides
b1610: tus - DNA replication terminus site-binding protein (NCBI), at 1,682,283 to 1,683,212
tus
b1611: fumC - fumarate hydratase (NCBI), at 1,683,209 to 1,684,612
fumC
b1612: fumA - fumarate hydratase (fumarase A), aerobic Class I (NCBI), at 1,684,755 to 1,686,401
fumA
Position (kb)
1683
1684
1685 Strain fitness (log2 ratio)
-1
0
1
2 at 1682.223 kb on + strand at 1682.223 kb on + strand at 1682.306 kb on - strand at 1682.451 kb on - strand, within tus at 1682.567 kb on - strand, within tus at 1682.620 kb on + strand, within tus at 1682.620 kb on + strand, within tus at 1682.820 kb on + strand, within tus at 1682.838 kb on + strand, within tus at 1682.878 kb on + strand, within tus at 1682.886 kb on - strand, within tus at 1682.941 kb on - strand, within tus at 1682.941 kb on - strand, within tus at 1683.046 kb on + strand, within tus at 1683.046 kb on + strand, within tus at 1683.207 kb on + strand at 1683.241 kb on + strand at 1683.295 kb on + strand at 1683.295 kb on + strand at 1683.439 kb on + strand, within fumC at 1683.595 kb on + strand, within fumC at 1683.612 kb on + strand, within fumC at 1683.612 kb on + strand, within fumC at 1683.665 kb on - strand, within fumC at 1683.665 kb on - strand, within fumC at 1683.709 kb on + strand, within fumC at 1683.709 kb on + strand, within fumC at 1683.859 kb on - strand, within fumC at 1683.859 kb on - strand, within fumC at 1683.981 kb on - strand, within fumC at 1684.016 kb on - strand, within fumC at 1684.016 kb on - strand, within fumC at 1684.247 kb on + strand, within fumC at 1684.247 kb on + strand, within fumC at 1684.600 kb on + strand at 1684.600 kb on + strand at 1685.163 kb on + strand, within fumA at 1685.193 kb on + strand, within fumA at 1685.290 kb on - strand, within fumA at 1685.290 kb on - strand, within fumA at 1685.310 kb on + strand, within fumA at 1685.344 kb on + strand, within fumA
Per-strain Table
Position Strand Gene LocusTag Fraction acetate (C) remove 1,682,223 + -0.3 1,682,223 + +0.6 1,682,306 - +0.8 1,682,451 - tus b1610 0.18 +0.9 1,682,567 - tus b1610 0.31 +2.1 1,682,620 + tus b1610 0.36 +1.3 1,682,620 + tus b1610 0.36 +0.8 1,682,820 + tus b1610 0.58 +0.7 1,682,838 + tus b1610 0.60 -1.1 1,682,878 + tus b1610 0.64 +0.5 1,682,886 - tus b1610 0.65 -0.9 1,682,941 - tus b1610 0.71 +0.8 1,682,941 - tus b1610 0.71 +0.8 1,683,046 + tus b1610 0.82 -0.1 1,683,046 + tus b1610 0.82 -0.2 1,683,207 + -0.1 1,683,241 + +0.8 1,683,295 + +0.4 1,683,295 + +0.8 1,683,439 + fumC b1611 0.16 +0.9 1,683,595 + fumC b1611 0.27 -1.2 1,683,612 + fumC b1611 0.29 +0.9 1,683,612 + fumC b1611 0.29 -0.1 1,683,665 - fumC b1611 0.32 +1.3 1,683,665 - fumC b1611 0.32 +1.3 1,683,709 + fumC b1611 0.36 +0.8 1,683,709 + fumC b1611 0.36 +0.1 1,683,859 - fumC b1611 0.46 +0.6 1,683,859 - fumC b1611 0.46 +2.4 1,683,981 - fumC b1611 0.55 +0.9 1,684,016 - fumC b1611 0.57 +1.1 1,684,016 - fumC b1611 0.57 +1.1 1,684,247 + fumC b1611 0.74 +0.9 1,684,247 + fumC b1611 0.74 +0.4 1,684,600 + +1.7 1,684,600 + +0.6 1,685,163 + fumA b1612 0.25 +0.2 1,685,193 + fumA b1612 0.27 +0.5 1,685,290 - fumA b1612 0.32 +0.1 1,685,290 - fumA b1612 0.32 -1.6 1,685,310 + fumA b1612 0.34 +0.5 1,685,344 + fumA b1612 0.36 +0.1
Or see this region's nucleotide sequence