Strain Fitness in Escherichia coli BW25113 around b0049

Experiment: acetate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntkefC and folA are separated by 191 nucleotidesfolA and apaH are separated by 77 nucleotidesapaH and apaG are separated by 6 nucleotidesapaG and ksgA are separated by 2 nucleotides b0047: kefC - glutathione-regulated potassium-efflux system protein (NCBI), at 47,769 to 49,631 kefC b0048: folA - dihydrofolate reductase (NCBI), at 49,823 to 50,302 folA b0049: apaH - diadenosinetetraphosphatase (NCBI), at 50,380 to 51,222 apaH b0050: apaG - hypothetical protein (NCBI), at 51,229 to 51,606 apaG b0051: ksgA - dimethyladenosine transferase (NCBI), at 51,609 to 52,430 ksgA Position (kb) 50 51 52Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 49.409 kb on - strand, within kefCat 49.431 kb on + strand, within kefCat 49.487 kb on - strandat 49.493 kb on - strandat 49.709 kb on + strandat 50.361 kb on + strandat 50.361 kb on + strandat 50.370 kb on - strandat 50.376 kb on + strandat 50.461 kb on + strandat 50.464 kb on - strandat 50.469 kb on - strand, within apaHat 50.501 kb on + strand, within apaHat 50.507 kb on - strand, within apaHat 50.568 kb on - strand, within apaHat 50.717 kb on - strand, within apaHat 50.785 kb on - strand, within apaHat 50.791 kb on + strand, within apaHat 50.802 kb on + strand, within apaHat 50.829 kb on - strand, within apaHat 50.859 kb on - strand, within apaHat 50.877 kb on + strand, within apaHat 50.877 kb on + strand, within apaHat 51.025 kb on + strand, within apaHat 51.109 kb on - strand, within apaHat 51.177 kb on + strandat 51.177 kb on + strandat 51.185 kb on - strandat 51.190 kb on + strandat 51.190 kb on + strandat 51.240 kb on + strandat 51.240 kb on + strandat 51.311 kb on + strand, within apaGat 51.311 kb on + strand, within apaGat 51.322 kb on - strand, within apaGat 51.326 kb on - strand, within apaGat 51.334 kb on - strand, within apaGat 51.336 kb on - strand, within apaGat 51.353 kb on + strand, within apaGat 51.353 kb on + strand, within apaGat 51.353 kb on - strand, within apaGat 51.364 kb on - strand, within apaGat 51.372 kb on - strand, within apaGat 51.415 kb on + strand, within apaGat 51.423 kb on - strand, within apaGat 51.466 kb on + strand, within apaGat 51.472 kb on + strand, within apaGat 51.512 kb on - strand, within apaGat 51.514 kb on - strand, within apaGat 51.520 kb on - strand, within apaGat 51.524 kb on + strand, within apaGat 51.550 kb on - strand, within apaGat 51.560 kb on + strand, within apaGat 51.567 kb on - strand, within apaGat 51.567 kb on - strand, within apaGat 51.576 kb on + strandat 51.576 kb on + strandat 51.586 kb on - strandat 51.613 kb on + strandat 51.614 kb on - strandat 51.615 kb on + strandat 51.615 kb on + strandat 51.673 kb on - strandat 51.703 kb on + strand, within ksgAat 51.703 kb on + strand, within ksgAat 51.812 kb on - strand, within ksgAat 51.836 kb on + strand, within ksgAat 51.891 kb on - strand, within ksgAat 51.891 kb on - strand, within ksgAat 51.904 kb on + strand, within ksgAat 51.906 kb on + strand, within ksgAat 51.925 kb on - strand, within ksgAat 52.040 kb on + strand, within ksgAat 52.040 kb on + strand, within ksgAat 52.040 kb on + strand, within ksgAat 52.042 kb on + strand, within ksgAat 52.042 kb on + strand, within ksgAat 52.042 kb on + strand, within ksgAat 52.042 kb on - strand, within ksgAat 52.042 kb on - strand, within ksgAat 52.045 kb on + strand, within ksgAat 52.062 kb on + strand, within ksgAat 52.062 kb on + strandat 52.070 kb on - strand, within ksgAat 52.072 kb on + strand, within ksgAat 52.089 kb on - strand, within ksgA

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Per-strain Table

Position Strand Gene LocusTag Fraction acetate (C)
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49,409 - kefC b0047 0.88 -0.0
49,431 + kefC b0047 0.89 +0.8
49,487 - -0.2
49,493 - +0.8
49,709 + -0.1
50,361 + +0.7
50,361 + -1.0
50,370 - +0.6
50,376 + +0.2
50,461 + -2.5
50,464 - -0.6
50,469 - apaH b0049 0.11 -3.0
50,501 + apaH b0049 0.14 -3.2
50,507 - apaH b0049 0.15 -1.1
50,568 - apaH b0049 0.22 -2.5
50,717 - apaH b0049 0.40 -2.8
50,785 - apaH b0049 0.48 -3.7
50,791 + apaH b0049 0.49 -1.2
50,802 + apaH b0049 0.50 -3.2
50,829 - apaH b0049 0.53 -0.2
50,859 - apaH b0049 0.57 -1.7
50,877 + apaH b0049 0.59 -0.6
50,877 + apaH b0049 0.59 -1.4
51,025 + apaH b0049 0.77 -2.5
51,109 - apaH b0049 0.86 -4.6
51,177 + -1.6
51,177 + -1.0
51,185 - -1.8
51,190 + -2.3
51,190 + -1.8
51,240 + -2.4
51,240 + -0.5
51,311 + apaG b0050 0.22 -1.0
51,311 + apaG b0050 0.22 -0.1
51,322 - apaG b0050 0.25 -0.6
51,326 - apaG b0050 0.26 -1.0
51,334 - apaG b0050 0.28 -1.6
51,336 - apaG b0050 0.28 +0.2
51,353 + apaG b0050 0.33 -2.1
51,353 + apaG b0050 0.33 -3.0
51,353 - apaG b0050 0.33 -0.7
51,364 - apaG b0050 0.36 -1.3
51,372 - apaG b0050 0.38 +0.3
51,415 + apaG b0050 0.49 -1.4
51,423 - apaG b0050 0.51 -0.7
51,466 + apaG b0050 0.63 +0.4
51,472 + apaG b0050 0.64 -2.8
51,512 - apaG b0050 0.75 +0.2
51,514 - apaG b0050 0.75 -0.2
51,520 - apaG b0050 0.77 +0.0
51,524 + apaG b0050 0.78 -0.5
51,550 - apaG b0050 0.85 -0.7
51,560 + apaG b0050 0.88 -1.0
51,567 - apaG b0050 0.89 -0.4
51,567 - apaG b0050 0.89 -1.0
51,576 + -2.6
51,576 + -2.6
51,586 - +0.2
51,613 + -0.6
51,614 - -0.4
51,615 + -0.7
51,615 + +0.3
51,673 - +1.0
51,703 + ksgA b0051 0.11 -0.0
51,703 + ksgA b0051 0.11 -1.7
51,812 - ksgA b0051 0.25 +1.2
51,836 + ksgA b0051 0.28 -1.2
51,891 - ksgA b0051 0.34 -0.0
51,891 - ksgA b0051 0.34 +0.7
51,904 + ksgA b0051 0.36 +0.2
51,906 + ksgA b0051 0.36 -1.0
51,925 - ksgA b0051 0.38 -2.1
52,040 + ksgA b0051 0.52 +0.2
52,040 + ksgA b0051 0.52 -1.6
52,040 + ksgA b0051 0.52 -1.7
52,042 + ksgA b0051 0.53 +0.7
52,042 + ksgA b0051 0.53 +0.2
52,042 + ksgA b0051 0.53 +1.0
52,042 - ksgA b0051 0.53 -0.8
52,042 - ksgA b0051 0.53 -0.1
52,045 + ksgA b0051 0.53 +0.1
52,062 + ksgA b0051 0.55 +1.0
52,062 + +0.2
52,070 - ksgA b0051 0.56 +0.7
52,072 + ksgA b0051 0.56 +0.8
52,089 - ksgA b0051 0.58 -2.8

Or see this region's nucleotide sequence