Strain Fitness in Escherichia coli BW25113 around b4013

Experiment: acetate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyjaA and yjaB are separated by 62 nucleotidesyjaB and metA are separated by 156 nucleotidesmetA and aceB are separated by 268 nucleotides b4011: yjaA - hypothetical protein (NCBI), at 4,211,257 to 4,211,640 yjaA b4012: yjaB - predicted acetyltransferase (NCBI), at 4,211,703 to 4,212,146 yjaB b4013: metA - homoserine O-succinyltransferase (NCBI), at 4,212,303 to 4,213,232 metA b4014: aceB - malate synthase (NCBI), at 4,213,501 to 4,215,102 aceB Position (kb) 4212 4213 4214Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 4211.358 kb on - strand, within yjaAat 4211.358 kb on - strand, within yjaAat 4211.402 kb on + strand, within yjaAat 4211.605 kb on - strandat 4211.659 kb on - strandat 4211.767 kb on - strand, within yjaBat 4211.973 kb on + strand, within yjaBat 4211.994 kb on - strand, within yjaBat 4211.994 kb on - strand, within yjaBat 4212.254 kb on - strandat 4212.689 kb on - strand, within metAat 4212.908 kb on - strand, within metAat 4212.908 kb on - strand, within metAat 4212.957 kb on + strand, within metAat 4213.008 kb on + strand, within metAat 4213.008 kb on + strand, within metAat 4213.008 kb on + strand, within metAat 4213.061 kb on - strand, within metAat 4213.068 kb on + strand, within metAat 4213.201 kb on + strandat 4213.491 kb on + strandat 4213.648 kb on + strandat 4213.765 kb on - strand, within aceBat 4213.777 kb on + strand, within aceBat 4213.777 kb on + strand, within aceBat 4213.976 kb on + strand, within aceBat 4214.093 kb on + strand, within aceBat 4214.104 kb on + strand, within aceBat 4214.109 kb on + strand, within aceBat 4214.182 kb on + strand, within aceBat 4214.190 kb on - strand, within aceB

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Per-strain Table

Position Strand Gene LocusTag Fraction acetate (C)
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4,211,358 - yjaA b4011 0.26 +0.4
4,211,358 - yjaA b4011 0.26 +0.5
4,211,402 + yjaA b4011 0.38 +0.8
4,211,605 - -1.3
4,211,659 - -0.7
4,211,767 - yjaB b4012 0.14 +0.5
4,211,973 + yjaB b4012 0.61 -3.5
4,211,994 - yjaB b4012 0.66 -0.8
4,211,994 - yjaB b4012 0.66 -0.4
4,212,254 - -1.4
4,212,689 - metA b4013 0.42 -1.4
4,212,908 - metA b4013 0.65 -2.1
4,212,908 - metA b4013 0.65 -2.4
4,212,957 + metA b4013 0.70 -1.5
4,213,008 + metA b4013 0.76 -1.9
4,213,008 + metA b4013 0.76 -1.4
4,213,008 + metA b4013 0.76 -0.9
4,213,061 - metA b4013 0.82 -1.5
4,213,068 + metA b4013 0.82 -2.9
4,213,201 + -5.1
4,213,491 + +0.9
4,213,648 + -0.2
4,213,765 - aceB b4014 0.16 -1.5
4,213,777 + aceB b4014 0.17 +0.8
4,213,777 + aceB b4014 0.17 -0.7
4,213,976 + aceB b4014 0.30 -2.3
4,214,093 + aceB b4014 0.37 -2.0
4,214,104 + aceB b4014 0.38 -3.0
4,214,109 + aceB b4014 0.38 +0.8
4,214,182 + aceB b4014 0.43 -3.7
4,214,190 - aceB b4014 0.43 -2.8

Or see this region's nucleotide sequence