Strain Fitness in Escherichia coli BW25113 around b2453

Experiment: acetate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nteutH and eutG are separated by 216 nucleotideseutG and eutJ overlap by 11 nucleotideseutJ and eutE are separated by 10 nucleotides b2452: eutH - predicted inner membrane protein (NCBI), at 2,564,903 to 2,566,129 eutH b2453: eutG - ethanolamine utilization; homolog of Salmonella enzyme, similar to iron-containing alcohol dehydrogenase (VIMSS), at 2,566,346 to 2,567,533 eutG b2454: eutJ - predicted chaperonin, ethanolamine utilization protein (NCBI), at 2,567,523 to 2,568,359 eutJ b2455: eutE - predicted aldehyde dehydrogenase, ethanolamine utilization protein (NCBI), at 2,568,370 to 2,569,773 eutE Position (kb) 2566 2567 2568Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2565.353 kb on - strand, within eutHat 2565.416 kb on + strand, within eutHat 2565.454 kb on - strand, within eutHat 2565.454 kb on - strand, within eutHat 2565.593 kb on + strand, within eutHat 2565.593 kb on + strand, within eutHat 2565.770 kb on - strand, within eutHat 2565.772 kb on - strand, within eutHat 2565.783 kb on - strand, within eutHat 2565.838 kb on - strand, within eutHat 2565.840 kb on + strand, within eutHat 2565.931 kb on + strand, within eutHat 2565.948 kb on + strand, within eutHat 2565.981 kb on + strand, within eutHat 2565.981 kb on + strand, within eutHat 2566.315 kb on + strandat 2566.315 kb on + strandat 2566.364 kb on + strandat 2566.409 kb on - strandat 2566.431 kb on + strandat 2566.451 kb on - strandat 2566.515 kb on + strand, within eutGat 2566.522 kb on + strand, within eutGat 2566.522 kb on + strand, within eutGat 2566.585 kb on + strand, within eutGat 2566.620 kb on + strand, within eutGat 2566.620 kb on + strand, within eutGat 2566.637 kb on + strand, within eutGat 2566.894 kb on + strand, within eutGat 2566.914 kb on + strand, within eutGat 2566.938 kb on - strand, within eutGat 2566.938 kb on - strand, within eutGat 2566.938 kb on - strand, within eutGat 2567.008 kb on + strand, within eutGat 2567.012 kb on - strand, within eutGat 2567.130 kb on + strand, within eutGat 2567.210 kb on + strand, within eutGat 2567.220 kb on + strand, within eutGat 2567.220 kb on + strand, within eutGat 2567.241 kb on + strand, within eutGat 2567.241 kb on - strand, within eutGat 2567.325 kb on - strand, within eutGat 2567.325 kb on - strand, within eutGat 2567.325 kb on - strand, within eutGat 2567.325 kb on - strand, within eutGat 2567.341 kb on + strand, within eutGat 2567.341 kb on + strand, within eutGat 2567.418 kb on - strandat 2567.595 kb on + strandat 2567.707 kb on + strand, within eutJat 2567.785 kb on + strand, within eutJat 2567.785 kb on + strand, within eutJat 2567.785 kb on + strand, within eutJat 2567.963 kb on + strand, within eutJat 2568.118 kb on - strand, within eutJat 2568.140 kb on - strand, within eutJat 2568.149 kb on + strand, within eutJat 2568.149 kb on + strand, within eutJat 2568.219 kb on + strand, within eutJat 2568.298 kb on + strandat 2568.338 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction acetate (C)
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2,565,353 - eutH b2452 0.37 +1.3
2,565,416 + eutH b2452 0.42 +0.1
2,565,454 - eutH b2452 0.45 +0.2
2,565,454 - eutH b2452 0.45 -2.4
2,565,593 + eutH b2452 0.56 -0.9
2,565,593 + eutH b2452 0.56 +0.3
2,565,770 - eutH b2452 0.71 -2.6
2,565,772 - eutH b2452 0.71 -0.4
2,565,783 - eutH b2452 0.72 -0.6
2,565,838 - eutH b2452 0.76 -0.3
2,565,840 + eutH b2452 0.76 +0.4
2,565,931 + eutH b2452 0.84 -0.8
2,565,948 + eutH b2452 0.85 -0.2
2,565,981 + eutH b2452 0.88 +0.2
2,565,981 + eutH b2452 0.88 +0.7
2,566,315 + -0.2
2,566,315 + -0.0
2,566,364 + +0.1
2,566,409 - +0.4
2,566,431 + +0.0
2,566,451 - -0.5
2,566,515 + eutG b2453 0.14 +0.1
2,566,522 + eutG b2453 0.15 +0.4
2,566,522 + eutG b2453 0.15 +0.1
2,566,585 + eutG b2453 0.20 +0.8
2,566,620 + eutG b2453 0.23 -0.1
2,566,620 + eutG b2453 0.23 +1.3
2,566,637 + eutG b2453 0.24 -2.6
2,566,894 + eutG b2453 0.46 -0.8
2,566,914 + eutG b2453 0.48 +0.2
2,566,938 - eutG b2453 0.50 +0.8
2,566,938 - eutG b2453 0.50 +0.3
2,566,938 - eutG b2453 0.50 +2.0
2,567,008 + eutG b2453 0.56 +0.0
2,567,012 - eutG b2453 0.56 +0.4
2,567,130 + eutG b2453 0.66 -0.7
2,567,210 + eutG b2453 0.73 +0.9
2,567,220 + eutG b2453 0.74 +0.4
2,567,220 + eutG b2453 0.74 -0.1
2,567,241 + eutG b2453 0.75 -3.5
2,567,241 - eutG b2453 0.75 -0.4
2,567,325 - eutG b2453 0.82 +1.2
2,567,325 - eutG b2453 0.82 -0.8
2,567,325 - eutG b2453 0.82 -0.3
2,567,325 - eutG b2453 0.82 -0.9
2,567,341 + eutG b2453 0.84 -3.0
2,567,341 + eutG b2453 0.84 -4.1
2,567,418 - -1.6
2,567,595 + +0.1
2,567,707 + eutJ b2454 0.22 +0.7
2,567,785 + eutJ b2454 0.31 +0.2
2,567,785 + eutJ b2454 0.31 +0.6
2,567,785 + eutJ b2454 0.31 +0.3
2,567,963 + eutJ b2454 0.53 -2.2
2,568,118 - eutJ b2454 0.71 +0.6
2,568,140 - eutJ b2454 0.74 +0.8
2,568,149 + eutJ b2454 0.75 +0.9
2,568,149 + eutJ b2454 0.75 -0.3
2,568,219 + eutJ b2454 0.83 +1.2
2,568,298 + +1.4
2,568,338 + +0.3

Or see this region's nucleotide sequence