Experiment: 4-Hydroxybenzoic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS08275 and ABID97_RS08280 overlap by 4 nucleotides ABID97_RS08280 and ABID97_RS08285 are separated by 82 nucleotides ABID97_RS08285 and ABID97_RS08290 are separated by 146 nucleotides ABID97_RS08290 and ABID97_RS08295 are separated by 61 nucleotides
ABID97_RS08275: ABID97_RS08275 - SAM-dependent methyltransferase, at 1,742,226 to 1,742,987
_RS08275
ABID97_RS08280: ABID97_RS08280 - Maf family nucleotide pyrophosphatase, at 1,742,984 to 1,743,565
_RS08280
ABID97_RS08285: ABID97_RS08285 - DUF177 domain-containing protein, at 1,743,648 to 1,744,205
_RS08285
ABID97_RS08290: ABID97_RS08290 - 50S ribosomal protein L32, at 1,744,352 to 1,744,534
_RS08290
ABID97_RS08295: ABID97_RS08295 - phosphate acyltransferase PlsX, at 1,744,596 to 1,745,693
_RS08295
Position (kb)
1743
1744
1745 Strain fitness (log2 ratio)
-2
-1
0
1 at 1742.833 kb on + strand, within ABID97_RS08275 at 1742.968 kb on + strand at 1742.969 kb on - strand at 1743.548 kb on + strand at 1743.549 kb on - strand at 1743.635 kb on + strand at 1743.636 kb on - strand at 1743.658 kb on + strand at 1743.658 kb on + strand at 1743.658 kb on + strand at 1743.659 kb on - strand at 1743.659 kb on - strand at 1743.659 kb on - strand at 1743.659 kb on - strand at 1743.659 kb on - strand at 1743.659 kb on - strand at 1743.738 kb on + strand, within ABID97_RS08285 at 1744.564 kb on + strand at 1744.601 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Hydroxybenzoic Acid (C) remove 1,742,833 + ABID97_RS08275 0.80 +1.0 1,742,968 + -0.2 1,742,969 - -0.1 1,743,548 + +0.2 1,743,549 - +0.2 1,743,635 + +0.8 1,743,636 - -1.0 1,743,658 + -0.5 1,743,658 + +0.7 1,743,658 + -0.2 1,743,659 - -0.0 1,743,659 - -0.4 1,743,659 - +0.6 1,743,659 - -1.0 1,743,659 - -1.3 1,743,659 - -0.1 1,743,738 + ABID97_RS08285 0.16 -2.5 1,744,564 + +0.8 1,744,601 + -1.1
Or see this region's nucleotide sequence