Experiment: KB with Erythromycin 6.25 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2274 and Psyr_2275 overlap by 4 nucleotides Psyr_2275 and Psyr_2276 are separated by 15 nucleotides
Psyr_2274: Psyr_2274 - glutamate synthase (NADPH) GltB1 subunit, at 2,640,302 to 2,641,222
_2274
Psyr_2275: Psyr_2275 - glutamate synthase (NADPH) GltB3 subunit, at 2,641,219 to 2,641,902
_2275
Psyr_2276: Psyr_2276 - glutamate synthase (NADPH) GltB2 subunit, at 2,641,918 to 2,643,258
_2276
Position (kb)
2641
2642 Strain fitness (log2 ratio)
-2
-1
0
1 at 2640.219 kb on + strand at 2640.220 kb on - strand at 2640.236 kb on + strand at 2640.236 kb on + strand at 2640.301 kb on + strand at 2640.301 kb on + strand at 2640.301 kb on + strand at 2640.301 kb on + strand at 2640.301 kb on + strand at 2640.302 kb on - strand at 2640.302 kb on - strand at 2640.323 kb on + strand at 2640.323 kb on + strand at 2640.324 kb on - strand at 2640.425 kb on + strand, within Psyr_2274 at 2640.426 kb on - strand, within Psyr_2274 at 2640.426 kb on - strand, within Psyr_2274 at 2640.426 kb on - strand, within Psyr_2274 at 2640.426 kb on - strand, within Psyr_2274 at 2640.486 kb on - strand, within Psyr_2274 at 2641.003 kb on + strand, within Psyr_2274 at 2641.091 kb on + strand, within Psyr_2274 at 2641.092 kb on - strand, within Psyr_2274 at 2641.092 kb on - strand, within Psyr_2274 at 2641.092 kb on - strand, within Psyr_2274 at 2641.092 kb on - strand, within Psyr_2274 at 2641.181 kb on + strand at 2641.181 kb on + strand at 2641.182 kb on - strand at 2641.182 kb on - strand at 2641.182 kb on - strand at 2641.182 kb on - strand at 2641.182 kb on - strand at 2641.182 kb on - strand at 2641.182 kb on - strand at 2641.182 kb on - strand at 2641.182 kb on - strand at 2641.182 kb on - strand at 2641.182 kb on - strand at 2641.182 kb on - strand at 2641.182 kb on - strand at 2641.182 kb on - strand at 2641.182 kb on - strand at 2641.182 kb on - strand at 2641.396 kb on + strand, within Psyr_2275 at 2641.396 kb on + strand, within Psyr_2275 at 2641.397 kb on - strand, within Psyr_2275 at 2641.699 kb on + strand, within Psyr_2275 at 2641.714 kb on + strand, within Psyr_2275 at 2641.714 kb on + strand, within Psyr_2275 at 2641.715 kb on - strand, within Psyr_2275 at 2641.867 kb on + strand at 2641.867 kb on + strand at 2641.868 kb on - strand at 2641.914 kb on + strand at 2641.929 kb on + strand at 2642.023 kb on + strand at 2642.024 kb on - strand at 2642.024 kb on - strand at 2642.024 kb on - strand at 2642.347 kb on + strand, within Psyr_2276 at 2642.490 kb on + strand, within Psyr_2276 at 2642.491 kb on - strand, within Psyr_2276 at 2642.491 kb on - strand, within Psyr_2276 at 2642.611 kb on + strand, within Psyr_2276 at 2642.612 kb on - strand, within Psyr_2276 at 2642.612 kb on - strand, within Psyr_2276 at 2642.612 kb on - strand, within Psyr_2276 at 2642.612 kb on - strand, within Psyr_2276 at 2642.612 kb on - strand, within Psyr_2276 at 2642.612 kb on - strand, within Psyr_2276 at 2642.641 kb on + strand, within Psyr_2276 at 2642.642 kb on - strand, within Psyr_2276
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Erythromycin 6.25 ug/ml remove 2,640,219 + +0.6 2,640,220 - -0.5 2,640,236 + -0.9 2,640,236 + -0.0 2,640,301 + -1.3 2,640,301 + +1.0 2,640,301 + +0.2 2,640,301 + -0.2 2,640,301 + -1.3 2,640,302 - -1.9 2,640,302 - -1.1 2,640,323 + -1.4 2,640,323 + +0.1 2,640,324 - -1.0 2,640,425 + Psyr_2274 0.13 +0.1 2,640,426 - Psyr_2274 0.13 +0.1 2,640,426 - Psyr_2274 0.13 +1.1 2,640,426 - Psyr_2274 0.13 +0.5 2,640,426 - Psyr_2274 0.13 -0.5 2,640,486 - Psyr_2274 0.20 -0.7 2,641,003 + Psyr_2274 0.76 +0.2 2,641,091 + Psyr_2274 0.86 +0.9 2,641,092 - Psyr_2274 0.86 -0.4 2,641,092 - Psyr_2274 0.86 -0.0 2,641,092 - Psyr_2274 0.86 +0.8 2,641,092 - Psyr_2274 0.86 +0.2 2,641,181 + +0.3 2,641,181 + -1.1 2,641,182 - +0.1 2,641,182 - -0.4 2,641,182 - +0.2 2,641,182 - -0.6 2,641,182 - +0.5 2,641,182 - -0.3 2,641,182 - -1.4 2,641,182 - +0.5 2,641,182 - +0.3 2,641,182 - -0.3 2,641,182 - -0.1 2,641,182 - -0.1 2,641,182 - +0.6 2,641,182 - +0.9 2,641,182 - +0.3 2,641,182 - -0.3 2,641,396 + Psyr_2275 0.26 -0.5 2,641,396 + Psyr_2275 0.26 -0.4 2,641,397 - Psyr_2275 0.26 -0.7 2,641,699 + Psyr_2275 0.70 -0.4 2,641,714 + Psyr_2275 0.72 -1.1 2,641,714 + Psyr_2275 0.72 -0.9 2,641,715 - Psyr_2275 0.73 -0.3 2,641,867 + -0.9 2,641,867 + -0.9 2,641,868 - -1.1 2,641,914 + +0.1 2,641,929 + -2.3 2,642,023 + +0.9 2,642,024 - -2.5 2,642,024 - +1.1 2,642,024 - -1.1 2,642,347 + Psyr_2276 0.32 -2.3 2,642,490 + Psyr_2276 0.43 +0.4 2,642,491 - Psyr_2276 0.43 -1.9 2,642,491 - Psyr_2276 0.43 -0.7 2,642,611 + Psyr_2276 0.52 +0.7 2,642,612 - Psyr_2276 0.52 -1.7 2,642,612 - Psyr_2276 0.52 -0.6 2,642,612 - Psyr_2276 0.52 -0.8 2,642,612 - Psyr_2276 0.52 +0.4 2,642,612 - Psyr_2276 0.52 +0.6 2,642,612 - Psyr_2276 0.52 +1.3 2,642,641 + Psyr_2276 0.54 +1.5 2,642,642 - Psyr_2276 0.54 -1.3
Or see this region's nucleotide sequence