Experiment: KB with Erythromycin 6.25 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0398 and Psyr_0399 are separated by 169 nucleotides Psyr_0399 and Psyr_0400 are separated by 13 nucleotides Psyr_0400 and Psyr_0401 are separated by 181 nucleotides
Psyr_0398: Psyr_0398 - replication restart DNA helicase PriA, at 425,511 to 427,730
_0398
Psyr_0399: Psyr_0399 - LSU ribosomal protein L31P, at 427,900 to 428,121
_0399
Psyr_0400: Psyr_0400 - nuclease (SNase-like), at 428,135 to 428,938
_0400
Psyr_0401: Psyr_0401 - Malate dehydrogenase (oxaloacetate decarboxylating) (NADP+), at 429,120 to 430,388
_0401
Position (kb) 428
429 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 427.751 kb on + strand at 427.752 kb on - strand at 427.752 kb on - strand at 427.775 kb on + strand at 427.775 kb on + strand at 427.776 kb on - strand at 427.838 kb on + strand at 427.861 kb on + strand at 427.861 kb on + strand at 427.861 kb on + strand at 427.861 kb on + strand at 427.895 kb on + strand at 428.029 kb on + strand, within Psyr_0399 at 428.120 kb on - strand at 428.120 kb on - strand at 428.326 kb on + strand, within Psyr_0400 at 428.326 kb on + strand, within Psyr_0400 at 428.327 kb on - strand, within Psyr_0400 at 428.460 kb on - strand, within Psyr_0400 at 428.483 kb on + strand, within Psyr_0400 at 428.484 kb on - strand, within Psyr_0400 at 428.484 kb on - strand, within Psyr_0400 at 428.987 kb on + strand at 428.988 kb on - strand at 428.988 kb on - strand at 428.988 kb on - strand at 428.988 kb on - strand at 428.998 kb on + strand at 428.998 kb on + strand at 429.003 kb on + strand at 429.004 kb on - strand at 429.004 kb on - strand at 429.010 kb on + strand at 429.011 kb on - strand at 429.011 kb on - strand at 429.011 kb on - strand at 429.052 kb on - strand at 429.057 kb on + strand at 429.057 kb on + strand at 429.057 kb on + strand at 429.057 kb on + strand at 429.058 kb on - strand at 429.058 kb on - strand at 429.058 kb on - strand at 429.058 kb on - strand at 429.058 kb on - strand at 429.117 kb on + strand at 429.117 kb on + strand at 429.117 kb on + strand at 429.117 kb on + strand at 429.118 kb on - strand at 429.118 kb on - strand at 429.118 kb on - strand at 429.118 kb on - strand at 429.118 kb on - strand at 429.118 kb on - strand at 429.231 kb on + strand at 429.288 kb on + strand, within Psyr_0401 at 429.288 kb on + strand, within Psyr_0401 at 429.288 kb on + strand, within Psyr_0401 at 429.288 kb on + strand, within Psyr_0401 at 429.289 kb on - strand, within Psyr_0401 at 429.289 kb on - strand, within Psyr_0401 at 429.294 kb on + strand, within Psyr_0401 at 429.295 kb on - strand, within Psyr_0401 at 429.295 kb on - strand, within Psyr_0401 at 429.317 kb on - strand, within Psyr_0401 at 429.317 kb on - strand, within Psyr_0401 at 429.323 kb on + strand, within Psyr_0401 at 429.323 kb on + strand, within Psyr_0401 at 429.323 kb on + strand, within Psyr_0401 at 429.324 kb on - strand, within Psyr_0401 at 429.395 kb on + strand, within Psyr_0401 at 429.492 kb on - strand, within Psyr_0401 at 429.820 kb on - strand, within Psyr_0401 at 429.820 kb on - strand, within Psyr_0401 at 429.886 kb on + strand, within Psyr_0401 at 429.886 kb on + strand, within Psyr_0401 at 429.887 kb on - strand, within Psyr_0401 at 429.887 kb on - strand, within Psyr_0401 at 429.892 kb on + strand, within Psyr_0401
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Erythromycin 6.25 ug/ml remove 427,751 + -0.2 427,752 - -2.0 427,752 - -1.3 427,775 + -2.4 427,775 + -0.2 427,776 - +1.1 427,838 + -0.7 427,861 + -1.5 427,861 + -0.9 427,861 + -1.2 427,861 + -3.2 427,895 + -1.2 428,029 + Psyr_0399 0.58 -1.2 428,120 - -0.6 428,120 - -2.5 428,326 + Psyr_0400 0.24 -0.9 428,326 + Psyr_0400 0.24 -0.8 428,327 - Psyr_0400 0.24 -1.1 428,460 - Psyr_0400 0.40 +0.2 428,483 + Psyr_0400 0.43 +1.0 428,484 - Psyr_0400 0.43 -1.4 428,484 - Psyr_0400 0.43 +0.4 428,987 + -0.5 428,988 - -1.0 428,988 - -0.5 428,988 - +0.7 428,988 - -0.5 428,998 + +0.9 428,998 + +0.7 429,003 + -1.5 429,004 - +0.9 429,004 - +0.2 429,010 + +0.5 429,011 - -0.6 429,011 - +0.8 429,011 - -1.3 429,052 - -0.1 429,057 + -0.3 429,057 + +0.8 429,057 + -0.7 429,057 + +0.4 429,058 - -1.3 429,058 - +0.4 429,058 - +0.4 429,058 - +0.8 429,058 - +0.3 429,117 + -1.2 429,117 + -0.1 429,117 + +0.8 429,117 + -0.5 429,118 - -0.5 429,118 - -0.7 429,118 - -0.5 429,118 - -0.8 429,118 - -0.2 429,118 - -0.6 429,231 + +1.1 429,288 + Psyr_0401 0.13 -0.6 429,288 + Psyr_0401 0.13 +0.1 429,288 + Psyr_0401 0.13 -0.2 429,288 + Psyr_0401 0.13 +0.5 429,289 - Psyr_0401 0.13 +0.6 429,289 - Psyr_0401 0.13 -0.6 429,294 + Psyr_0401 0.14 -1.7 429,295 - Psyr_0401 0.14 -2.0 429,295 - Psyr_0401 0.14 +0.4 429,317 - Psyr_0401 0.16 -0.7 429,317 - Psyr_0401 0.16 -0.3 429,323 + Psyr_0401 0.16 -0.5 429,323 + Psyr_0401 0.16 +0.9 429,323 + Psyr_0401 0.16 -0.3 429,324 - Psyr_0401 0.16 -0.3 429,395 + Psyr_0401 0.22 +0.8 429,492 - Psyr_0401 0.29 +0.3 429,820 - Psyr_0401 0.55 -1.9 429,820 - Psyr_0401 0.55 -0.5 429,886 + Psyr_0401 0.60 +0.5 429,886 + Psyr_0401 0.60 +1.3 429,887 - Psyr_0401 0.60 -0.9 429,887 - Psyr_0401 0.60 -0.2 429,892 + Psyr_0401 0.61 -0.5
Or see this region's nucleotide sequence