Experiment: L-Valine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_1483 and PfGW456L13_1484 overlap by 4 nucleotides PfGW456L13_1484 and PfGW456L13_1485 are separated by 25 nucleotides PfGW456L13_1485 and PfGW456L13_1486 are separated by 34 nucleotides PfGW456L13_1486 and PfGW456L13_1487 are separated by 14 nucleotides PfGW456L13_1487 and PfGW456L13_1488 are separated by 56 nucleotides
PfGW456L13_1483 - Type IV fimbrial biogenesis protein PilV, at 1,635,612 to 1,636,028
_1483
PfGW456L13_1484 - Type IV fimbrial biogenesis protein PilW, at 1,636,025 to 1,636,732
_1484
PfGW456L13_1485 - Type IV fimbrial biogenesis protein PilX, at 1,636,758 to 1,637,222
_1485
PfGW456L13_1486 - hypothetical protein, at 1,637,257 to 1,637,622
_1486
PfGW456L13_1487 - Type IV pilus biogenesis protein PilE, at 1,637,637 to 1,638,038
_1487
PfGW456L13_1488 - Glycine oxidase ThiO (EC 1.4.3.19), at 1,638,095 to 1,639,195
_1488
Position (kb)
1636
1637
1638 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1635.786 kb on + strand, within PfGW456L13_1483 at 1635.787 kb on - strand, within PfGW456L13_1483 at 1635.787 kb on - strand, within PfGW456L13_1483 at 1635.787 kb on - strand, within PfGW456L13_1483 at 1635.918 kb on + strand, within PfGW456L13_1483 at 1635.918 kb on + strand, within PfGW456L13_1483 at 1635.918 kb on + strand, within PfGW456L13_1483 at 1635.918 kb on + strand, within PfGW456L13_1483 at 1635.918 kb on + strand at 1635.918 kb on + strand, within PfGW456L13_1483 at 1635.919 kb on - strand, within PfGW456L13_1483 at 1635.919 kb on - strand, within PfGW456L13_1483 at 1635.919 kb on - strand, within PfGW456L13_1483 at 1635.919 kb on - strand, within PfGW456L13_1483 at 1635.919 kb on - strand, within PfGW456L13_1483 at 1635.919 kb on - strand, within PfGW456L13_1483 at 1635.919 kb on - strand, within PfGW456L13_1483 at 1635.919 kb on - strand, within PfGW456L13_1483 at 1635.919 kb on - strand, within PfGW456L13_1483 at 1635.929 kb on + strand, within PfGW456L13_1483 at 1635.929 kb on + strand, within PfGW456L13_1483 at 1635.929 kb on + strand, within PfGW456L13_1483 at 1635.929 kb on + strand, within PfGW456L13_1483 at 1635.929 kb on + strand, within PfGW456L13_1483 at 1635.930 kb on - strand, within PfGW456L13_1483 at 1635.930 kb on - strand, within PfGW456L13_1483 at 1635.930 kb on - strand, within PfGW456L13_1483 at 1635.930 kb on - strand, within PfGW456L13_1483 at 1635.930 kb on - strand, within PfGW456L13_1483 at 1635.930 kb on - strand, within PfGW456L13_1483 at 1635.930 kb on - strand, within PfGW456L13_1483 at 1635.930 kb on - strand, within PfGW456L13_1483 at 1635.932 kb on + strand, within PfGW456L13_1483 at 1635.932 kb on + strand, within PfGW456L13_1483 at 1635.933 kb on - strand, within PfGW456L13_1483 at 1635.959 kb on + strand, within PfGW456L13_1483 at 1635.959 kb on + strand, within PfGW456L13_1483 at 1636.049 kb on - strand at 1636.136 kb on + strand, within PfGW456L13_1484 at 1636.136 kb on + strand, within PfGW456L13_1484 at 1636.136 kb on + strand, within PfGW456L13_1484 at 1636.136 kb on + strand, within PfGW456L13_1484 at 1636.136 kb on + strand, within PfGW456L13_1484 at 1636.136 kb on + strand, within PfGW456L13_1484 at 1636.137 kb on - strand, within PfGW456L13_1484 at 1636.137 kb on - strand, within PfGW456L13_1484 at 1636.137 kb on - strand, within PfGW456L13_1484 at 1636.137 kb on - strand, within PfGW456L13_1484 at 1636.137 kb on - strand, within PfGW456L13_1484 at 1636.288 kb on - strand, within PfGW456L13_1484 at 1636.390 kb on + strand, within PfGW456L13_1484 at 1636.391 kb on - strand, within PfGW456L13_1484 at 1636.391 kb on - strand, within PfGW456L13_1484 at 1636.409 kb on + strand, within PfGW456L13_1484 at 1636.409 kb on + strand, within PfGW456L13_1484 at 1636.409 kb on + strand, within PfGW456L13_1484 at 1636.409 kb on + strand, within PfGW456L13_1484 at 1636.409 kb on + strand, within PfGW456L13_1484 at 1636.410 kb on - strand, within PfGW456L13_1484 at 1636.410 kb on - strand, within PfGW456L13_1484 at 1636.410 kb on - strand, within PfGW456L13_1484 at 1636.469 kb on + strand, within PfGW456L13_1484 at 1636.470 kb on - strand, within PfGW456L13_1484 at 1636.524 kb on + strand, within PfGW456L13_1484 at 1636.524 kb on + strand, within PfGW456L13_1484 at 1636.601 kb on + strand, within PfGW456L13_1484 at 1636.637 kb on - strand, within PfGW456L13_1484 at 1636.637 kb on - strand, within PfGW456L13_1484 at 1636.767 kb on + strand at 1636.767 kb on + strand at 1636.767 kb on + strand at 1636.767 kb on + strand at 1636.768 kb on - strand at 1636.768 kb on - strand at 1636.877 kb on + strand, within PfGW456L13_1485 at 1636.877 kb on + strand, within PfGW456L13_1485 at 1636.940 kb on + strand, within PfGW456L13_1485 at 1636.941 kb on - strand, within PfGW456L13_1485 at 1636.959 kb on + strand, within PfGW456L13_1485 at 1636.959 kb on + strand, within PfGW456L13_1485 at 1636.959 kb on + strand, within PfGW456L13_1485 at 1636.960 kb on - strand, within PfGW456L13_1485 at 1636.960 kb on - strand, within PfGW456L13_1485 at 1636.960 kb on - strand, within PfGW456L13_1485 at 1636.960 kb on - strand, within PfGW456L13_1485 at 1636.966 kb on + strand, within PfGW456L13_1485 at 1636.967 kb on - strand, within PfGW456L13_1485 at 1637.018 kb on + strand, within PfGW456L13_1485 at 1637.018 kb on + strand, within PfGW456L13_1485 at 1637.019 kb on - strand, within PfGW456L13_1485 at 1637.082 kb on + strand, within PfGW456L13_1485 at 1637.082 kb on + strand, within PfGW456L13_1485 at 1637.082 kb on + strand, within PfGW456L13_1485 at 1637.152 kb on - strand, within PfGW456L13_1485 at 1637.152 kb on - strand, within PfGW456L13_1485 at 1637.167 kb on + strand, within PfGW456L13_1485 at 1637.167 kb on + strand, within PfGW456L13_1485 at 1637.168 kb on - strand, within PfGW456L13_1485 at 1637.208 kb on + strand at 1637.208 kb on + strand at 1637.208 kb on + strand at 1637.208 kb on + strand at 1637.208 kb on + strand at 1637.208 kb on + strand at 1637.209 kb on - strand at 1637.209 kb on - strand at 1637.209 kb on - strand at 1637.209 kb on - strand at 1637.209 kb on - strand at 1637.209 kb on - strand at 1637.217 kb on + strand at 1637.217 kb on + strand at 1637.217 kb on + strand at 1637.230 kb on - strand at 1637.357 kb on - strand, within PfGW456L13_1486 at 1637.365 kb on + strand, within PfGW456L13_1486 at 1637.365 kb on + strand, within PfGW456L13_1486 at 1637.365 kb on + strand, within PfGW456L13_1486 at 1637.366 kb on - strand, within PfGW456L13_1486 at 1637.366 kb on - strand, within PfGW456L13_1486 at 1637.366 kb on - strand, within PfGW456L13_1486 at 1637.424 kb on + strand, within PfGW456L13_1486 at 1637.424 kb on + strand, within PfGW456L13_1486 at 1637.425 kb on - strand, within PfGW456L13_1486 at 1637.425 kb on - strand, within PfGW456L13_1486 at 1637.425 kb on - strand, within PfGW456L13_1486 at 1637.486 kb on + strand, within PfGW456L13_1486 at 1637.615 kb on + strand at 1637.615 kb on + strand at 1637.615 kb on + strand at 1637.615 kb on + strand at 1637.615 kb on + strand at 1637.615 kb on + strand at 1637.615 kb on + strand at 1637.616 kb on - strand at 1637.616 kb on - strand at 1637.616 kb on - strand at 1637.616 kb on - strand at 1637.616 kb on - strand at 1637.616 kb on - strand at 1637.620 kb on + strand at 1637.620 kb on + strand at 1637.620 kb on + strand at 1637.620 kb on + strand at 1637.620 kb on + strand at 1637.620 kb on + strand at 1637.620 kb on + strand at 1637.621 kb on - strand at 1637.621 kb on - strand at 1637.621 kb on - strand at 1637.621 kb on - strand at 1637.621 kb on - strand at 1637.621 kb on - strand at 1637.621 kb on - strand at 1637.621 kb on - strand at 1637.621 kb on - strand at 1637.621 kb on - strand at 1637.642 kb on + strand at 1637.642 kb on + strand at 1637.642 kb on + strand at 1637.642 kb on + strand at 1637.643 kb on - strand at 1637.645 kb on + strand at 1637.645 kb on + strand at 1637.646 kb on - strand at 1637.646 kb on - strand at 1637.646 kb on - strand at 1637.646 kb on - strand at 1637.661 kb on - strand at 1637.736 kb on + strand, within PfGW456L13_1487 at 1637.809 kb on - strand, within PfGW456L13_1487 at 1637.809 kb on - strand, within PfGW456L13_1487 at 1637.809 kb on - strand, within PfGW456L13_1487 at 1637.826 kb on + strand, within PfGW456L13_1487 at 1637.826 kb on + strand, within PfGW456L13_1487 at 1637.827 kb on - strand, within PfGW456L13_1487 at 1637.862 kb on + strand, within PfGW456L13_1487 at 1637.862 kb on + strand, within PfGW456L13_1487 at 1637.862 kb on + strand, within PfGW456L13_1487 at 1637.862 kb on + strand, within PfGW456L13_1487 at 1637.862 kb on + strand, within PfGW456L13_1487 at 1637.862 kb on + strand, within PfGW456L13_1487 at 1637.862 kb on + strand, within PfGW456L13_1487 at 1637.862 kb on + strand, within PfGW456L13_1487 at 1637.863 kb on - strand, within PfGW456L13_1487 at 1637.863 kb on - strand, within PfGW456L13_1487 at 1637.863 kb on - strand, within PfGW456L13_1487 at 1637.865 kb on + strand, within PfGW456L13_1487 at 1637.865 kb on + strand, within PfGW456L13_1487 at 1637.865 kb on + strand, within PfGW456L13_1487 at 1637.865 kb on + strand, within PfGW456L13_1487 at 1637.865 kb on + strand, within PfGW456L13_1487 at 1637.865 kb on + strand, within PfGW456L13_1487 at 1637.865 kb on + strand, within PfGW456L13_1487 at 1637.866 kb on - strand, within PfGW456L13_1487 at 1637.866 kb on - strand, within PfGW456L13_1487 at 1637.866 kb on - strand, within PfGW456L13_1487 at 1637.866 kb on - strand, within PfGW456L13_1487 at 1637.918 kb on + strand, within PfGW456L13_1487 at 1637.919 kb on - strand, within PfGW456L13_1487 at 1637.919 kb on - strand, within PfGW456L13_1487 at 1637.919 kb on - strand, within PfGW456L13_1487 at 1637.980 kb on + strand, within PfGW456L13_1487 at 1637.980 kb on + strand, within PfGW456L13_1487 at 1637.980 kb on + strand, within PfGW456L13_1487 at 1637.980 kb on + strand, within PfGW456L13_1487 at 1637.980 kb on + strand, within PfGW456L13_1487 at 1637.981 kb on - strand, within PfGW456L13_1487 at 1637.981 kb on - strand, within PfGW456L13_1487 at 1637.981 kb on - strand, within PfGW456L13_1487 at 1638.044 kb on + strand at 1638.044 kb on + strand at 1638.062 kb on + strand at 1638.062 kb on + strand at 1638.073 kb on + strand at 1638.073 kb on + strand at 1638.074 kb on - strand at 1638.074 kb on - strand at 1638.074 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (C) remove 1,635,786 + PfGW456L13_1483 0.42 -0.5 1,635,787 - PfGW456L13_1483 0.42 -1.7 1,635,787 - PfGW456L13_1483 0.42 -1.9 1,635,787 - PfGW456L13_1483 0.42 +0.3 1,635,918 + PfGW456L13_1483 0.73 -0.7 1,635,918 + PfGW456L13_1483 0.73 +0.5 1,635,918 + PfGW456L13_1483 0.73 +0.6 1,635,918 + PfGW456L13_1483 0.73 +0.6 1,635,918 + -0.4 1,635,918 + PfGW456L13_1483 0.73 +1.0 1,635,919 - PfGW456L13_1483 0.74 -0.8 1,635,919 - PfGW456L13_1483 0.74 -0.7 1,635,919 - PfGW456L13_1483 0.74 +1.2 1,635,919 - PfGW456L13_1483 0.74 -0.0 1,635,919 - PfGW456L13_1483 0.74 -2.1 1,635,919 - PfGW456L13_1483 0.74 -0.2 1,635,919 - PfGW456L13_1483 0.74 -2.0 1,635,919 - PfGW456L13_1483 0.74 +0.1 1,635,919 - PfGW456L13_1483 0.74 -0.1 1,635,929 + PfGW456L13_1483 0.76 +0.1 1,635,929 + PfGW456L13_1483 0.76 +0.8 1,635,929 + PfGW456L13_1483 0.76 +1.0 1,635,929 + PfGW456L13_1483 0.76 -2.3 1,635,929 + PfGW456L13_1483 0.76 -0.5 1,635,930 - PfGW456L13_1483 0.76 +0.5 1,635,930 - PfGW456L13_1483 0.76 +0.8 1,635,930 - PfGW456L13_1483 0.76 +0.1 1,635,930 - PfGW456L13_1483 0.76 -0.6 1,635,930 - PfGW456L13_1483 0.76 +1.6 1,635,930 - PfGW456L13_1483 0.76 -0.9 1,635,930 - PfGW456L13_1483 0.76 +0.7 1,635,930 - PfGW456L13_1483 0.76 -0.3 1,635,932 + PfGW456L13_1483 0.77 +0.5 1,635,932 + PfGW456L13_1483 0.77 +0.4 1,635,933 - PfGW456L13_1483 0.77 -2.0 1,635,959 + PfGW456L13_1483 0.83 -0.5 1,635,959 + PfGW456L13_1483 0.83 -0.3 1,636,049 - +0.8 1,636,136 + PfGW456L13_1484 0.16 +0.6 1,636,136 + PfGW456L13_1484 0.16 +0.7 1,636,136 + PfGW456L13_1484 0.16 -1.0 1,636,136 + PfGW456L13_1484 0.16 -0.6 1,636,136 + PfGW456L13_1484 0.16 +1.0 1,636,136 + PfGW456L13_1484 0.16 -1.5 1,636,137 - PfGW456L13_1484 0.16 +0.2 1,636,137 - PfGW456L13_1484 0.16 -0.0 1,636,137 - PfGW456L13_1484 0.16 -0.4 1,636,137 - PfGW456L13_1484 0.16 +0.3 1,636,137 - PfGW456L13_1484 0.16 +0.4 1,636,288 - PfGW456L13_1484 0.37 +0.8 1,636,390 + PfGW456L13_1484 0.52 +1.0 1,636,391 - PfGW456L13_1484 0.52 -0.8 1,636,391 - PfGW456L13_1484 0.52 +0.7 1,636,409 + PfGW456L13_1484 0.54 +0.6 1,636,409 + PfGW456L13_1484 0.54 +1.1 1,636,409 + PfGW456L13_1484 0.54 -2.7 1,636,409 + PfGW456L13_1484 0.54 +0.2 1,636,409 + PfGW456L13_1484 0.54 +1.1 1,636,410 - PfGW456L13_1484 0.54 +0.0 1,636,410 - PfGW456L13_1484 0.54 -1.0 1,636,410 - PfGW456L13_1484 0.54 -1.3 1,636,469 + PfGW456L13_1484 0.63 +0.1 1,636,470 - PfGW456L13_1484 0.63 -0.1 1,636,524 + PfGW456L13_1484 0.70 +0.1 1,636,524 + PfGW456L13_1484 0.70 +0.5 1,636,601 + PfGW456L13_1484 0.81 -2.0 1,636,637 - PfGW456L13_1484 0.86 -0.4 1,636,637 - PfGW456L13_1484 0.86 +0.4 1,636,767 + +0.6 1,636,767 + -0.9 1,636,767 + -0.4 1,636,767 + -0.3 1,636,768 - +0.7 1,636,768 - +0.8 1,636,877 + PfGW456L13_1485 0.26 -0.8 1,636,877 + PfGW456L13_1485 0.26 +0.8 1,636,940 + PfGW456L13_1485 0.39 +0.1 1,636,941 - PfGW456L13_1485 0.39 -0.8 1,636,959 + PfGW456L13_1485 0.43 -2.0 1,636,959 + PfGW456L13_1485 0.43 +1.3 1,636,959 + PfGW456L13_1485 0.43 +0.1 1,636,960 - PfGW456L13_1485 0.43 -1.0 1,636,960 - PfGW456L13_1485 0.43 +0.2 1,636,960 - PfGW456L13_1485 0.43 -0.6 1,636,960 - PfGW456L13_1485 0.43 +0.7 1,636,966 + PfGW456L13_1485 0.45 -1.2 1,636,967 - PfGW456L13_1485 0.45 +0.2 1,637,018 + PfGW456L13_1485 0.56 -1.1 1,637,018 + PfGW456L13_1485 0.56 +1.5 1,637,019 - PfGW456L13_1485 0.56 +0.6 1,637,082 + PfGW456L13_1485 0.70 +2.2 1,637,082 + PfGW456L13_1485 0.70 -1.5 1,637,082 + PfGW456L13_1485 0.70 -0.4 1,637,152 - PfGW456L13_1485 0.85 +0.3 1,637,152 - PfGW456L13_1485 0.85 +0.3 1,637,167 + PfGW456L13_1485 0.88 -1.1 1,637,167 + PfGW456L13_1485 0.88 +0.7 1,637,168 - PfGW456L13_1485 0.88 -2.3 1,637,208 + +1.0 1,637,208 + +0.3 1,637,208 + +0.6 1,637,208 + +1.2 1,637,208 + +0.8 1,637,208 + +0.2 1,637,209 - -0.1 1,637,209 - +0.7 1,637,209 - +0.1 1,637,209 - +0.6 1,637,209 - -0.7 1,637,209 - -0.6 1,637,217 + -0.7 1,637,217 + -0.3 1,637,217 + -0.3 1,637,230 - +1.3 1,637,357 - PfGW456L13_1486 0.27 +0.2 1,637,365 + PfGW456L13_1486 0.30 -0.7 1,637,365 + PfGW456L13_1486 0.30 +0.5 1,637,365 + PfGW456L13_1486 0.30 -1.4 1,637,366 - PfGW456L13_1486 0.30 -0.2 1,637,366 - PfGW456L13_1486 0.30 -0.4 1,637,366 - PfGW456L13_1486 0.30 -0.4 1,637,424 + PfGW456L13_1486 0.46 +0.4 1,637,424 + PfGW456L13_1486 0.46 -0.4 1,637,425 - PfGW456L13_1486 0.46 -0.5 1,637,425 - PfGW456L13_1486 0.46 +1.2 1,637,425 - PfGW456L13_1486 0.46 +0.7 1,637,486 + PfGW456L13_1486 0.63 -2.7 1,637,615 + +0.3 1,637,615 + +0.5 1,637,615 + -0.8 1,637,615 + -1.0 1,637,615 + +0.0 1,637,615 + +1.2 1,637,615 + +1.6 1,637,616 - +1.5 1,637,616 - -1.1 1,637,616 - -0.6 1,637,616 - +0.3 1,637,616 - -0.1 1,637,616 - -0.0 1,637,620 + -0.7 1,637,620 + +1.8 1,637,620 + +1.7 1,637,620 + +0.6 1,637,620 + +1.0 1,637,620 + +0.6 1,637,620 + -0.3 1,637,621 - -0.6 1,637,621 - -0.3 1,637,621 - -2.6 1,637,621 - +0.7 1,637,621 - -2.9 1,637,621 - +1.1 1,637,621 - -0.5 1,637,621 - +1.0 1,637,621 - -1.3 1,637,621 - +0.7 1,637,642 + -0.8 1,637,642 + -3.4 1,637,642 + -0.4 1,637,642 + +0.1 1,637,643 - +1.1 1,637,645 + -0.7 1,637,645 + +0.6 1,637,646 - -1.0 1,637,646 - -1.7 1,637,646 - -0.1 1,637,646 - -1.0 1,637,661 - -0.6 1,637,736 + PfGW456L13_1487 0.25 +0.3 1,637,809 - PfGW456L13_1487 0.43 +1.1 1,637,809 - PfGW456L13_1487 0.43 +0.7 1,637,809 - PfGW456L13_1487 0.43 +1.0 1,637,826 + PfGW456L13_1487 0.47 +0.4 1,637,826 + PfGW456L13_1487 0.47 +0.5 1,637,827 - PfGW456L13_1487 0.47 -0.7 1,637,862 + PfGW456L13_1487 0.56 +0.9 1,637,862 + PfGW456L13_1487 0.56 -0.8 1,637,862 + PfGW456L13_1487 0.56 +1.1 1,637,862 + PfGW456L13_1487 0.56 +1.0 1,637,862 + PfGW456L13_1487 0.56 +0.9 1,637,862 + PfGW456L13_1487 0.56 +0.4 1,637,862 + PfGW456L13_1487 0.56 +0.1 1,637,862 + PfGW456L13_1487 0.56 -0.2 1,637,863 - PfGW456L13_1487 0.56 -0.3 1,637,863 - PfGW456L13_1487 0.56 +1.5 1,637,863 - PfGW456L13_1487 0.56 +0.5 1,637,865 + PfGW456L13_1487 0.57 +0.8 1,637,865 + PfGW456L13_1487 0.57 +0.7 1,637,865 + PfGW456L13_1487 0.57 +0.9 1,637,865 + PfGW456L13_1487 0.57 -0.6 1,637,865 + PfGW456L13_1487 0.57 -0.3 1,637,865 + PfGW456L13_1487 0.57 +0.8 1,637,865 + PfGW456L13_1487 0.57 -0.3 1,637,866 - PfGW456L13_1487 0.57 -1.5 1,637,866 - PfGW456L13_1487 0.57 -0.3 1,637,866 - PfGW456L13_1487 0.57 -1.2 1,637,866 - PfGW456L13_1487 0.57 -0.7 1,637,918 + PfGW456L13_1487 0.70 -1.4 1,637,919 - PfGW456L13_1487 0.70 -0.4 1,637,919 - PfGW456L13_1487 0.70 +0.5 1,637,919 - PfGW456L13_1487 0.70 +1.5 1,637,980 + PfGW456L13_1487 0.85 -0.3 1,637,980 + PfGW456L13_1487 0.85 +0.8 1,637,980 + PfGW456L13_1487 0.85 -0.6 1,637,980 + PfGW456L13_1487 0.85 -0.3 1,637,980 + PfGW456L13_1487 0.85 +0.2 1,637,981 - PfGW456L13_1487 0.86 +1.7 1,637,981 - PfGW456L13_1487 0.86 -0.9 1,637,981 - PfGW456L13_1487 0.86 -0.1 1,638,044 + -1.2 1,638,044 + -2.0 1,638,062 + -0.8 1,638,062 + -0.5 1,638,073 + +0.1 1,638,073 + -1.1 1,638,074 - -0.4 1,638,074 - -0.3 1,638,074 - -0.3
Or see this region's nucleotide sequence