Experiment: PYE with Dimethyl Sulfoxide 0.4 %
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00222 and CCNA_00223 are separated by 8 nucleotides CCNA_00223 and CCNA_00224 overlap by 4 nucleotides CCNA_00224 and CCNA_00225 are separated by 330 nucleotides CCNA_00225 and CCNA_00226 are separated by 100 nucleotides CCNA_00226 and CCNA_00227 are separated by 57 nucleotides
CCNA_00222: CCNA_00222 - glucose-6-phosphate isomerase/glucose-6 phosphate 1-epimerase, at 238,241 to 239,860
_00222
CCNA_00223: CCNA_00223 - esterase lipase Family protein, at 239,869 to 240,651
_00223
CCNA_00224: CCNA_00224 - TonB-dependent receptor protein, at 240,648 to 242,693
_00224
CCNA_00225: CCNA_00225 - hypothetical protein, at 243,024 to 243,056
_00225
CCNA_00226: CCNA_00226 - hypothetical protein, at 243,157 to 243,432
_00226
CCNA_00227: CCNA_00227 - methyltransferase, at 243,490 to 244,056
_00227
Position (kb) 240
241
242
243 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 239.854 kb on + strand at 239.859 kb on + strand at 239.915 kb on - strand at 239.947 kb on + strand at 240.014 kb on - strand, within CCNA_00223 at 240.058 kb on - strand, within CCNA_00223 at 240.060 kb on + strand, within CCNA_00223 at 240.061 kb on + strand, within CCNA_00223 at 240.074 kb on - strand, within CCNA_00223 at 240.082 kb on + strand, within CCNA_00223 at 240.090 kb on - strand, within CCNA_00223 at 240.113 kb on + strand, within CCNA_00223 at 240.134 kb on + strand, within CCNA_00223 at 240.154 kb on + strand, within CCNA_00223 at 240.157 kb on - strand, within CCNA_00223 at 240.162 kb on - strand, within CCNA_00223 at 240.194 kb on - strand, within CCNA_00223 at 240.199 kb on + strand, within CCNA_00223 at 240.199 kb on + strand, within CCNA_00223 at 240.262 kb on + strand, within CCNA_00223 at 240.283 kb on - strand, within CCNA_00223 at 240.299 kb on + strand, within CCNA_00223 at 240.307 kb on - strand, within CCNA_00223 at 240.312 kb on + strand, within CCNA_00223 at 240.312 kb on + strand, within CCNA_00223 at 240.312 kb on + strand, within CCNA_00223 at 240.312 kb on + strand, within CCNA_00223 at 240.312 kb on + strand, within CCNA_00223 at 240.312 kb on + strand, within CCNA_00223 at 240.318 kb on + strand, within CCNA_00223 at 240.318 kb on + strand, within CCNA_00223 at 240.318 kb on + strand, within CCNA_00223 at 240.318 kb on + strand, within CCNA_00223 at 240.318 kb on + strand, within CCNA_00223 at 240.320 kb on - strand, within CCNA_00223 at 240.320 kb on - strand, within CCNA_00223 at 240.326 kb on - strand, within CCNA_00223 at 240.326 kb on - strand, within CCNA_00223 at 240.326 kb on - strand, within CCNA_00223 at 240.326 kb on - strand, within CCNA_00223 at 240.397 kb on + strand, within CCNA_00223 at 240.402 kb on + strand, within CCNA_00223 at 240.407 kb on + strand, within CCNA_00223 at 240.407 kb on + strand, within CCNA_00223 at 240.410 kb on - strand, within CCNA_00223 at 240.410 kb on - strand, within CCNA_00223 at 240.424 kb on + strand, within CCNA_00223 at 240.430 kb on - strand, within CCNA_00223 at 240.431 kb on - strand, within CCNA_00223 at 240.440 kb on + strand, within CCNA_00223 at 240.440 kb on + strand, within CCNA_00223 at 240.456 kb on + strand, within CCNA_00223 at 240.456 kb on + strand, within CCNA_00223 at 240.468 kb on + strand, within CCNA_00223 at 240.468 kb on + strand, within CCNA_00223 at 240.468 kb on + strand, within CCNA_00223 at 240.468 kb on + strand, within CCNA_00223 at 240.498 kb on + strand, within CCNA_00223 at 240.506 kb on - strand, within CCNA_00223 at 240.506 kb on - strand, within CCNA_00223 at 240.511 kb on - strand, within CCNA_00223 at 240.511 kb on - strand, within CCNA_00223 at 240.534 kb on - strand, within CCNA_00223 at 240.539 kb on - strand, within CCNA_00223 at 240.575 kb on + strand at 240.615 kb on + strand at 240.682 kb on - strand at 240.682 kb on - strand at 240.702 kb on + strand at 240.702 kb on + strand at 240.702 kb on + strand at 240.707 kb on + strand at 240.710 kb on - strand at 240.788 kb on - strand at 240.804 kb on + strand at 240.812 kb on - strand at 240.822 kb on - strand at 240.822 kb on - strand at 240.827 kb on - strand at 240.840 kb on + strand at 240.841 kb on - strand at 240.848 kb on - strand at 240.848 kb on - strand at 240.864 kb on + strand, within CCNA_00224 at 240.867 kb on - strand, within CCNA_00224 at 240.872 kb on - strand, within CCNA_00224 at 240.872 kb on - strand, within CCNA_00224 at 240.877 kb on - strand, within CCNA_00224 at 240.925 kb on + strand, within CCNA_00224 at 240.925 kb on + strand, within CCNA_00224 at 240.925 kb on + strand, within CCNA_00224 at 240.933 kb on - strand, within CCNA_00224 at 240.947 kb on + strand, within CCNA_00224 at 240.947 kb on + strand, within CCNA_00224 at 240.958 kb on + strand, within CCNA_00224 at 240.963 kb on + strand, within CCNA_00224 at 240.971 kb on - strand, within CCNA_00224 at 241.014 kb on - strand, within CCNA_00224 at 241.017 kb on + strand, within CCNA_00224 at 241.049 kb on + strand, within CCNA_00224 at 241.050 kb on + strand, within CCNA_00224 at 241.063 kb on + strand, within CCNA_00224 at 241.088 kb on + strand, within CCNA_00224 at 241.121 kb on - strand, within CCNA_00224 at 241.261 kb on + strand, within CCNA_00224 at 241.276 kb on + strand, within CCNA_00224 at 241.327 kb on - strand, within CCNA_00224 at 241.345 kb on + strand, within CCNA_00224 at 241.402 kb on + strand, within CCNA_00224 at 241.428 kb on - strand, within CCNA_00224 at 241.440 kb on - strand, within CCNA_00224 at 241.446 kb on + strand, within CCNA_00224 at 241.462 kb on + strand, within CCNA_00224 at 241.486 kb on + strand, within CCNA_00224 at 241.493 kb on + strand, within CCNA_00224 at 241.510 kb on + strand, within CCNA_00224 at 241.522 kb on + strand, within CCNA_00224 at 241.537 kb on - strand, within CCNA_00224 at 241.540 kb on + strand, within CCNA_00224 at 241.553 kb on - strand, within CCNA_00224 at 241.575 kb on - strand, within CCNA_00224 at 241.593 kb on - strand, within CCNA_00224 at 241.616 kb on - strand, within CCNA_00224 at 241.644 kb on + strand, within CCNA_00224 at 241.644 kb on + strand, within CCNA_00224 at 241.665 kb on + strand, within CCNA_00224 at 241.675 kb on + strand, within CCNA_00224 at 241.681 kb on + strand, within CCNA_00224 at 241.728 kb on + strand, within CCNA_00224 at 241.744 kb on + strand, within CCNA_00224 at 241.757 kb on + strand, within CCNA_00224 at 241.762 kb on + strand, within CCNA_00224 at 241.771 kb on - strand, within CCNA_00224 at 241.826 kb on - strand, within CCNA_00224 at 241.843 kb on + strand, within CCNA_00224 at 241.843 kb on + strand, within CCNA_00224 at 241.866 kb on + strand, within CCNA_00224 at 241.866 kb on + strand, within CCNA_00224 at 241.866 kb on + strand, within CCNA_00224 at 241.867 kb on + strand, within CCNA_00224 at 241.867 kb on + strand, within CCNA_00224 at 241.874 kb on - strand, within CCNA_00224 at 241.875 kb on - strand, within CCNA_00224 at 241.957 kb on + strand, within CCNA_00224 at 242.025 kb on - strand, within CCNA_00224 at 242.092 kb on + strand, within CCNA_00224 at 242.095 kb on - strand, within CCNA_00224 at 242.122 kb on + strand, within CCNA_00224 at 242.130 kb on - strand, within CCNA_00224 at 242.172 kb on - strand, within CCNA_00224 at 242.216 kb on + strand, within CCNA_00224 at 242.216 kb on + strand, within CCNA_00224 at 242.216 kb on + strand, within CCNA_00224 at 242.224 kb on - strand, within CCNA_00224 at 242.224 kb on - strand, within CCNA_00224 at 242.229 kb on + strand, within CCNA_00224 at 242.285 kb on + strand, within CCNA_00224 at 242.285 kb on - strand, within CCNA_00224 at 242.299 kb on - strand, within CCNA_00224 at 242.299 kb on - strand, within CCNA_00224 at 242.343 kb on + strand, within CCNA_00224 at 242.365 kb on + strand, within CCNA_00224 at 242.390 kb on - strand, within CCNA_00224 at 242.409 kb on + strand, within CCNA_00224 at 242.428 kb on - strand, within CCNA_00224 at 242.438 kb on + strand, within CCNA_00224 at 242.445 kb on + strand, within CCNA_00224 at 242.473 kb on - strand, within CCNA_00224 at 242.481 kb on + strand, within CCNA_00224 at 242.489 kb on - strand at 242.489 kb on - strand at 242.489 kb on - strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.535 kb on - strand at 242.535 kb on - strand at 242.535 kb on - strand at 242.585 kb on - strand at 242.587 kb on + strand at 242.643 kb on + strand at 242.643 kb on + strand at 242.667 kb on + strand at 242.687 kb on - strand at 242.699 kb on + strand at 242.701 kb on + strand at 242.709 kb on - strand at 242.715 kb on + strand at 242.715 kb on + strand at 242.715 kb on + strand at 242.715 kb on + strand at 242.892 kb on + strand at 242.894 kb on + strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.942 kb on + strand at 242.965 kb on + strand at 242.971 kb on + strand at 242.973 kb on - strand at 243.010 kb on - strand at 243.015 kb on - strand at 243.051 kb on + strand, within CCNA_00225 at 243.057 kb on - strand at 243.083 kb on + strand at 243.091 kb on - strand at 243.116 kb on + strand at 243.154 kb on + strand at 243.162 kb on - strand at 243.162 kb on - strand at 243.172 kb on - strand at 243.177 kb on + strand at 243.198 kb on + strand, within CCNA_00226 at 243.198 kb on + strand, within CCNA_00226 at 243.198 kb on + strand, within CCNA_00226 at 243.206 kb on - strand, within CCNA_00226 at 243.206 kb on - strand, within CCNA_00226 at 243.213 kb on + strand, within CCNA_00226 at 243.275 kb on + strand, within CCNA_00226 at 243.288 kb on - strand, within CCNA_00226 at 243.304 kb on + strand, within CCNA_00226 at 243.305 kb on + strand, within CCNA_00226 at 243.324 kb on + strand, within CCNA_00226 at 243.379 kb on - strand, within CCNA_00226 at 243.408 kb on - strand at 243.449 kb on + strand at 243.460 kb on - strand at 243.462 kb on + strand at 243.495 kb on - strand at 243.544 kb on + strand at 243.644 kb on + strand, within CCNA_00227 at 243.658 kb on + strand, within CCNA_00227
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Dimethyl Sulfoxide 0.4 % remove 239,854 + -0.4 239,859 + -0.3 239,915 - +0.6 239,947 + -0.0 240,014 - CCNA_00223 0.19 -0.7 240,058 - CCNA_00223 0.24 -1.3 240,060 + CCNA_00223 0.24 -0.1 240,061 + CCNA_00223 0.25 +0.7 240,074 - CCNA_00223 0.26 -0.6 240,082 + CCNA_00223 0.27 +0.1 240,090 - CCNA_00223 0.28 +1.0 240,113 + CCNA_00223 0.31 +0.3 240,134 + CCNA_00223 0.34 -0.3 240,154 + CCNA_00223 0.36 -0.1 240,157 - CCNA_00223 0.37 +0.5 240,162 - CCNA_00223 0.37 -0.6 240,194 - CCNA_00223 0.42 -0.6 240,199 + CCNA_00223 0.42 -0.5 240,199 + CCNA_00223 0.42 -0.2 240,262 + CCNA_00223 0.50 -0.3 240,283 - CCNA_00223 0.53 +0.3 240,299 + CCNA_00223 0.55 -0.1 240,307 - CCNA_00223 0.56 -0.2 240,312 + CCNA_00223 0.57 +0.4 240,312 + CCNA_00223 0.57 -0.1 240,312 + CCNA_00223 0.57 +0.0 240,312 + CCNA_00223 0.57 -0.4 240,312 + CCNA_00223 0.57 +0.3 240,312 + CCNA_00223 0.57 -0.3 240,318 + CCNA_00223 0.57 -0.4 240,318 + CCNA_00223 0.57 +0.9 240,318 + CCNA_00223 0.57 +0.5 240,318 + CCNA_00223 0.57 +0.2 240,318 + CCNA_00223 0.57 +1.7 240,320 - CCNA_00223 0.58 -0.8 240,320 - CCNA_00223 0.58 -1.8 240,326 - CCNA_00223 0.58 -0.5 240,326 - CCNA_00223 0.58 +0.1 240,326 - CCNA_00223 0.58 -0.1 240,326 - CCNA_00223 0.58 +0.8 240,397 + CCNA_00223 0.67 +0.1 240,402 + CCNA_00223 0.68 +0.2 240,407 + CCNA_00223 0.69 +0.2 240,407 + CCNA_00223 0.69 -0.1 240,410 - CCNA_00223 0.69 -0.7 240,410 - CCNA_00223 0.69 -0.3 240,424 + CCNA_00223 0.71 -1.8 240,430 - CCNA_00223 0.72 -0.0 240,431 - CCNA_00223 0.72 -0.6 240,440 + CCNA_00223 0.73 +0.1 240,440 + CCNA_00223 0.73 +0.3 240,456 + CCNA_00223 0.75 +0.2 240,456 + CCNA_00223 0.75 +0.3 240,468 + CCNA_00223 0.77 -1.0 240,468 + CCNA_00223 0.77 +1.0 240,468 + CCNA_00223 0.77 +0.1 240,468 + CCNA_00223 0.77 +0.2 240,498 + CCNA_00223 0.80 +0.9 240,506 - CCNA_00223 0.81 +0.0 240,506 - CCNA_00223 0.81 -0.8 240,511 - CCNA_00223 0.82 -0.5 240,511 - CCNA_00223 0.82 -1.5 240,534 - CCNA_00223 0.85 +1.0 240,539 - CCNA_00223 0.86 +0.0 240,575 + +1.1 240,615 + +0.3 240,682 - +0.1 240,682 - -1.2 240,702 + -0.3 240,702 + -1.7 240,702 + -0.3 240,707 + -0.6 240,710 - +0.6 240,788 - -0.2 240,804 + -0.4 240,812 - -0.7 240,822 - -0.5 240,822 - -0.0 240,827 - +0.1 240,840 + -0.4 240,841 - +0.5 240,848 - +0.2 240,848 - -0.2 240,864 + CCNA_00224 0.11 -3.3 240,867 - CCNA_00224 0.11 -0.4 240,872 - CCNA_00224 0.11 -0.9 240,872 - CCNA_00224 0.11 +0.1 240,877 - CCNA_00224 0.11 +0.2 240,925 + CCNA_00224 0.14 -0.2 240,925 + CCNA_00224 0.14 -0.5 240,925 + CCNA_00224 0.14 +1.2 240,933 - CCNA_00224 0.14 -0.1 240,947 + CCNA_00224 0.15 -0.6 240,947 + CCNA_00224 0.15 -1.6 240,958 + CCNA_00224 0.15 -0.8 240,963 + CCNA_00224 0.15 -0.2 240,971 - CCNA_00224 0.16 +0.6 241,014 - CCNA_00224 0.18 -0.3 241,017 + CCNA_00224 0.18 +0.1 241,049 + CCNA_00224 0.20 +0.1 241,050 + CCNA_00224 0.20 -0.2 241,063 + CCNA_00224 0.20 +0.4 241,088 + CCNA_00224 0.22 +1.0 241,121 - CCNA_00224 0.23 -0.9 241,261 + CCNA_00224 0.30 -0.4 241,276 + CCNA_00224 0.31 -0.1 241,327 - CCNA_00224 0.33 -1.7 241,345 + CCNA_00224 0.34 +0.6 241,402 + CCNA_00224 0.37 +0.3 241,428 - CCNA_00224 0.38 -0.3 241,440 - CCNA_00224 0.39 +0.5 241,446 + CCNA_00224 0.39 +0.0 241,462 + CCNA_00224 0.40 -0.6 241,486 + CCNA_00224 0.41 +0.1 241,493 + CCNA_00224 0.41 -0.7 241,510 + CCNA_00224 0.42 -1.1 241,522 + CCNA_00224 0.43 +0.0 241,537 - CCNA_00224 0.43 -0.9 241,540 + CCNA_00224 0.44 -0.5 241,553 - CCNA_00224 0.44 -0.1 241,575 - CCNA_00224 0.45 -0.0 241,593 - CCNA_00224 0.46 +0.3 241,616 - CCNA_00224 0.47 -0.6 241,644 + CCNA_00224 0.49 -0.3 241,644 + CCNA_00224 0.49 -0.1 241,665 + CCNA_00224 0.50 -0.4 241,675 + CCNA_00224 0.50 +0.3 241,681 + CCNA_00224 0.50 -1.2 241,728 + CCNA_00224 0.53 -0.7 241,744 + CCNA_00224 0.54 -2.1 241,757 + CCNA_00224 0.54 -0.2 241,762 + CCNA_00224 0.54 +0.4 241,771 - CCNA_00224 0.55 -0.4 241,826 - CCNA_00224 0.58 +0.0 241,843 + CCNA_00224 0.58 -1.2 241,843 + CCNA_00224 0.58 -1.0 241,866 + CCNA_00224 0.60 +0.6 241,866 + CCNA_00224 0.60 -1.0 241,866 + CCNA_00224 0.60 -0.7 241,867 + CCNA_00224 0.60 -1.5 241,867 + CCNA_00224 0.60 -1.2 241,874 - CCNA_00224 0.60 -2.0 241,875 - CCNA_00224 0.60 -0.4 241,957 + CCNA_00224 0.64 -0.7 242,025 - CCNA_00224 0.67 -0.8 242,092 + CCNA_00224 0.71 +0.2 242,095 - CCNA_00224 0.71 +2.3 242,122 + CCNA_00224 0.72 -1.8 242,130 - CCNA_00224 0.72 +0.0 242,172 - CCNA_00224 0.74 +0.2 242,216 + CCNA_00224 0.77 +0.4 242,216 + CCNA_00224 0.77 -0.3 242,216 + CCNA_00224 0.77 +1.2 242,224 - CCNA_00224 0.77 +0.0 242,224 - CCNA_00224 0.77 -0.3 242,229 + CCNA_00224 0.77 -1.2 242,285 + CCNA_00224 0.80 -0.9 242,285 - CCNA_00224 0.80 +1.1 242,299 - CCNA_00224 0.81 +0.2 242,299 - CCNA_00224 0.81 +0.2 242,343 + CCNA_00224 0.83 -0.5 242,365 + CCNA_00224 0.84 -0.2 242,390 - CCNA_00224 0.85 -0.2 242,409 + CCNA_00224 0.86 +0.6 242,428 - CCNA_00224 0.87 -1.6 242,438 + CCNA_00224 0.87 +0.6 242,445 + CCNA_00224 0.88 -0.3 242,473 - CCNA_00224 0.89 +0.9 242,481 + CCNA_00224 0.90 -1.0 242,489 - +0.4 242,489 - -0.1 242,489 - -0.8 242,527 + +0.4 242,527 + -0.7 242,527 + +0.3 242,535 - +0.7 242,535 - -1.0 242,535 - -1.3 242,585 - +0.8 242,587 + +0.3 242,643 + -1.2 242,643 + -1.5 242,667 + -1.1 242,687 - -0.6 242,699 + +0.6 242,701 + +0.3 242,709 - +0.6 242,715 + -0.2 242,715 + -1.5 242,715 + +0.5 242,715 + -0.0 242,892 + -1.0 242,894 + +0.8 242,900 - +0.3 242,900 - -0.0 242,900 - -0.3 242,900 - +0.3 242,900 - -2.3 242,900 - +0.1 242,900 - +0.1 242,900 - -0.3 242,900 - -0.6 242,900 - +0.2 242,900 - +0.0 242,942 + -0.1 242,965 + -0.1 242,971 + -0.5 242,973 - -0.7 243,010 - -1.2 243,015 - +0.2 243,051 + CCNA_00225 0.82 +0.4 243,057 - -0.4 243,083 + +0.1 243,091 - -0.7 243,116 + -1.4 243,154 + +0.2 243,162 - +0.4 243,162 - +0.6 243,172 - +0.3 243,177 + +0.1 243,198 + CCNA_00226 0.15 -0.3 243,198 + CCNA_00226 0.15 -0.6 243,198 + CCNA_00226 0.15 -0.1 243,206 - CCNA_00226 0.18 -0.4 243,206 - CCNA_00226 0.18 +0.4 243,213 + CCNA_00226 0.20 +1.0 243,275 + CCNA_00226 0.43 +0.8 243,288 - CCNA_00226 0.47 -1.6 243,304 + CCNA_00226 0.53 -0.7 243,305 + CCNA_00226 0.54 +0.4 243,324 + CCNA_00226 0.61 +0.0 243,379 - CCNA_00226 0.80 +0.5 243,408 - -0.0 243,449 + -1.4 243,460 - -0.6 243,462 + +0.6 243,495 - +0.6 243,544 + -1.6 243,644 + CCNA_00227 0.27 -0.9 243,658 + CCNA_00227 0.30 -0.2
Or see this region's nucleotide sequence