Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02267 and CCNA_02268 overlap by 50 nucleotides CCNA_02268 and CCNA_R0053 are separated by 214 nucleotides CCNA_R0053 and CCNA_02269 are separated by 101 nucleotides
CCNA_02267: CCNA_02267 - serine hydrolase family protein, at 2,420,364 to 2,420,987
_02267
CCNA_02268: CCNA_02268 - laminin G domain protein, at 2,420,938 to 2,421,585
_02268
CCNA_R0053: CCNA_R0053 - tRNA-Leu, at 2,421,800 to 2,421,884
_R0053
CCNA_02269: CCNA_02269 - L-aminopeptidase/D-esterase dmpA, at 2,421,986 to 2,422,978
_02269
Position (kb)
2421
2422 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2420.844 kb on + strand, within CCNA_02267 at 2420.849 kb on + strand, within CCNA_02267 at 2420.853 kb on + strand, within CCNA_02267 at 2420.903 kb on - strand, within CCNA_02267 at 2420.903 kb on - strand, within CCNA_02267 at 2420.936 kb on + strand at 2421.041 kb on - strand, within CCNA_02268 at 2421.055 kb on + strand, within CCNA_02268 at 2421.063 kb on - strand, within CCNA_02268 at 2421.069 kb on + strand, within CCNA_02268 at 2421.077 kb on - strand, within CCNA_02268 at 2421.079 kb on + strand, within CCNA_02268 at 2421.099 kb on + strand, within CCNA_02268 at 2421.100 kb on - strand, within CCNA_02268 at 2421.118 kb on + strand, within CCNA_02268 at 2421.139 kb on + strand, within CCNA_02268 at 2421.147 kb on - strand, within CCNA_02268 at 2421.147 kb on - strand, within CCNA_02268 at 2421.147 kb on - strand, within CCNA_02268 at 2421.152 kb on + strand, within CCNA_02268 at 2421.234 kb on + strand, within CCNA_02268 at 2421.236 kb on + strand, within CCNA_02268 at 2421.236 kb on + strand, within CCNA_02268 at 2421.236 kb on + strand, within CCNA_02268 at 2421.236 kb on + strand, within CCNA_02268 at 2421.237 kb on - strand, within CCNA_02268 at 2421.244 kb on - strand, within CCNA_02268 at 2421.244 kb on - strand, within CCNA_02268 at 2421.249 kb on - strand, within CCNA_02268 at 2421.307 kb on - strand, within CCNA_02268 at 2421.307 kb on - strand, within CCNA_02268 at 2421.334 kb on - strand, within CCNA_02268 at 2421.382 kb on - strand, within CCNA_02268 at 2421.383 kb on - strand, within CCNA_02268 at 2421.392 kb on - strand, within CCNA_02268 at 2421.474 kb on + strand, within CCNA_02268 at 2421.589 kb on - strand at 2421.625 kb on + strand at 2421.630 kb on + strand at 2421.695 kb on + strand at 2421.956 kb on + strand at 2421.956 kb on + strand at 2421.956 kb on + strand at 2421.964 kb on - strand at 2421.988 kb on + strand at 2421.996 kb on - strand at 2422.029 kb on + strand at 2422.037 kb on - strand at 2422.071 kb on + strand at 2422.084 kb on + strand at 2422.085 kb on - strand at 2422.089 kb on + strand, within CCNA_02269 at 2422.097 kb on - strand, within CCNA_02269 at 2422.097 kb on - strand, within CCNA_02269 at 2422.097 kb on - strand, within CCNA_02269 at 2422.110 kb on + strand, within CCNA_02269 at 2422.128 kb on - strand, within CCNA_02269 at 2422.243 kb on + strand, within CCNA_02269 at 2422.243 kb on + strand, within CCNA_02269 at 2422.245 kb on + strand, within CCNA_02269 at 2422.245 kb on + strand, within CCNA_02269 at 2422.245 kb on + strand, within CCNA_02269 at 2422.245 kb on + strand, within CCNA_02269 at 2422.253 kb on - strand, within CCNA_02269 at 2422.253 kb on - strand, within CCNA_02269 at 2422.336 kb on + strand, within CCNA_02269 at 2422.357 kb on - strand, within CCNA_02269 at 2422.362 kb on - strand, within CCNA_02269 at 2422.454 kb on - strand, within CCNA_02269 at 2422.558 kb on - strand, within CCNA_02269 at 2422.635 kb on + strand, within CCNA_02269 at 2422.659 kb on + strand, within CCNA_02269 at 2422.731 kb on + strand, within CCNA_02269 at 2422.783 kb on - strand, within CCNA_02269 at 2422.799 kb on + strand, within CCNA_02269
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 2,420,844 + CCNA_02267 0.77 +0.1 2,420,849 + CCNA_02267 0.78 -0.7 2,420,853 + CCNA_02267 0.78 -1.0 2,420,903 - CCNA_02267 0.86 +0.0 2,420,903 - CCNA_02267 0.86 -0.2 2,420,936 + -1.4 2,421,041 - CCNA_02268 0.16 -0.2 2,421,055 + CCNA_02268 0.18 -1.0 2,421,063 - CCNA_02268 0.19 -0.4 2,421,069 + CCNA_02268 0.20 +0.6 2,421,077 - CCNA_02268 0.21 +0.1 2,421,079 + CCNA_02268 0.22 -0.9 2,421,099 + CCNA_02268 0.25 -0.4 2,421,100 - CCNA_02268 0.25 +0.4 2,421,118 + CCNA_02268 0.28 +0.0 2,421,139 + CCNA_02268 0.31 -1.9 2,421,147 - CCNA_02268 0.32 +0.4 2,421,147 - CCNA_02268 0.32 -0.8 2,421,147 - CCNA_02268 0.32 +0.6 2,421,152 + CCNA_02268 0.33 -1.1 2,421,234 + CCNA_02268 0.46 +0.4 2,421,236 + CCNA_02268 0.46 -0.7 2,421,236 + CCNA_02268 0.46 -0.0 2,421,236 + CCNA_02268 0.46 +0.7 2,421,236 + CCNA_02268 0.46 +0.3 2,421,237 - CCNA_02268 0.46 +0.1 2,421,244 - CCNA_02268 0.47 +0.1 2,421,244 - CCNA_02268 0.47 -0.8 2,421,249 - CCNA_02268 0.48 +0.8 2,421,307 - CCNA_02268 0.57 -0.6 2,421,307 - CCNA_02268 0.57 -0.4 2,421,334 - CCNA_02268 0.61 -0.1 2,421,382 - CCNA_02268 0.69 +3.1 2,421,383 - CCNA_02268 0.69 +0.7 2,421,392 - CCNA_02268 0.70 -2.0 2,421,474 + CCNA_02268 0.83 -0.6 2,421,589 - -2.1 2,421,625 + -0.4 2,421,630 + -0.5 2,421,695 + +1.5 2,421,956 + -0.1 2,421,956 + +0.5 2,421,956 + +0.2 2,421,964 - +0.9 2,421,988 + -1.7 2,421,996 - -0.3 2,422,029 + +0.9 2,422,037 - +1.0 2,422,071 + +1.2 2,422,084 + -0.1 2,422,085 - -0.2 2,422,089 + CCNA_02269 0.10 +0.3 2,422,097 - CCNA_02269 0.11 +0.5 2,422,097 - CCNA_02269 0.11 -1.1 2,422,097 - CCNA_02269 0.11 +1.2 2,422,110 + CCNA_02269 0.12 -1.5 2,422,128 - CCNA_02269 0.14 +0.0 2,422,243 + CCNA_02269 0.26 +0.3 2,422,243 + CCNA_02269 0.26 +1.7 2,422,245 + CCNA_02269 0.26 -0.8 2,422,245 + CCNA_02269 0.26 -0.1 2,422,245 + CCNA_02269 0.26 -0.0 2,422,245 + CCNA_02269 0.26 -1.3 2,422,253 - CCNA_02269 0.27 -0.6 2,422,253 - CCNA_02269 0.27 +0.6 2,422,336 + CCNA_02269 0.35 -0.5 2,422,357 - CCNA_02269 0.37 -1.5 2,422,362 - CCNA_02269 0.38 +0.0 2,422,454 - CCNA_02269 0.47 -0.8 2,422,558 - CCNA_02269 0.58 -0.3 2,422,635 + CCNA_02269 0.65 +1.1 2,422,659 + CCNA_02269 0.68 +0.6 2,422,731 + CCNA_02269 0.75 +0.5 2,422,783 - CCNA_02269 0.80 -0.8 2,422,799 + CCNA_02269 0.82 -0.0
Or see this region's nucleotide sequence