Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02221 and CCNA_03963 overlap by 11 nucleotides CCNA_03963 and CCNA_02222 overlap by 1 nucleotides
CCNA_02221: CCNA_02221 - methionine synthase I metH, at 2,367,923 to 2,370,622
_02221
CCNA_03963: CCNA_03963 - hypothetical protein, at 2,370,612 to 2,370,800
_03963
CCNA_02222: CCNA_02222 - 5-methyltetrahydrofolate--homocysteine methyltransferase homocysteine-binding subunit, at 2,370,800 to 2,371,876
_02222
Position (kb)
2370
2371 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2369.646 kb on - strand, within CCNA_02221 at 2369.671 kb on - strand, within CCNA_02221 at 2369.696 kb on + strand, within CCNA_02221 at 2369.704 kb on - strand, within CCNA_02221 at 2369.709 kb on - strand, within CCNA_02221 at 2369.730 kb on + strand, within CCNA_02221 at 2369.730 kb on + strand, within CCNA_02221 at 2369.743 kb on + strand, within CCNA_02221 at 2369.743 kb on + strand, within CCNA_02221 at 2369.743 kb on + strand, within CCNA_02221 at 2369.756 kb on - strand, within CCNA_02221 at 2369.766 kb on + strand, within CCNA_02221 at 2369.939 kb on - strand, within CCNA_02221 at 2370.038 kb on - strand, within CCNA_02221 at 2370.090 kb on + strand, within CCNA_02221 at 2370.123 kb on + strand, within CCNA_02221 at 2370.156 kb on + strand, within CCNA_02221 at 2370.164 kb on + strand, within CCNA_02221 at 2370.164 kb on + strand, within CCNA_02221 at 2370.172 kb on - strand, within CCNA_02221 at 2370.212 kb on + strand, within CCNA_02221 at 2370.213 kb on + strand, within CCNA_02221 at 2370.213 kb on + strand, within CCNA_02221 at 2370.229 kb on - strand, within CCNA_02221 at 2370.229 kb on - strand, within CCNA_02221 at 2370.257 kb on - strand, within CCNA_02221 at 2370.291 kb on - strand, within CCNA_02221 at 2370.322 kb on - strand, within CCNA_02221 at 2370.326 kb on + strand, within CCNA_02221 at 2370.326 kb on + strand, within CCNA_02221 at 2370.334 kb on - strand, within CCNA_02221 at 2370.334 kb on - strand, within CCNA_02221 at 2370.336 kb on + strand, within CCNA_02221 at 2370.336 kb on + strand, within CCNA_02221 at 2370.486 kb on + strand at 2370.486 kb on + strand at 2370.494 kb on - strand at 2370.570 kb on + strand at 2370.593 kb on + strand at 2370.706 kb on + strand, within CCNA_03963 at 2370.740 kb on - strand, within CCNA_03963 at 2370.747 kb on - strand, within CCNA_03963 at 2370.766 kb on + strand, within CCNA_03963 at 2370.808 kb on + strand at 2370.840 kb on + strand at 2370.840 kb on + strand at 2370.840 kb on + strand at 2370.840 kb on + strand at 2370.840 kb on + strand at 2370.848 kb on - strand at 2370.848 kb on - strand at 2370.848 kb on - strand at 2370.848 kb on - strand at 2370.848 kb on - strand at 2370.848 kb on - strand at 2370.848 kb on - strand at 2370.907 kb on + strand at 2370.908 kb on + strand, within CCNA_02222 at 2370.908 kb on + strand, within CCNA_02222 at 2370.949 kb on + strand, within CCNA_02222 at 2370.965 kb on + strand, within CCNA_02222 at 2371.017 kb on + strand, within CCNA_02222 at 2371.037 kb on + strand, within CCNA_02222 at 2371.094 kb on + strand, within CCNA_02222 at 2371.126 kb on - strand, within CCNA_02222 at 2371.165 kb on - strand, within CCNA_02222 at 2371.252 kb on + strand, within CCNA_02222 at 2371.277 kb on + strand, within CCNA_02222 at 2371.307 kb on - strand, within CCNA_02222 at 2371.332 kb on + strand, within CCNA_02222 at 2371.342 kb on + strand, within CCNA_02222 at 2371.374 kb on + strand, within CCNA_02222 at 2371.374 kb on + strand, within CCNA_02222 at 2371.374 kb on + strand, within CCNA_02222 at 2371.374 kb on + strand, within CCNA_02222 at 2371.382 kb on - strand, within CCNA_02222 at 2371.467 kb on + strand, within CCNA_02222 at 2371.528 kb on + strand, within CCNA_02222 at 2371.573 kb on + strand, within CCNA_02222 at 2371.596 kb on - strand, within CCNA_02222 at 2371.619 kb on - strand, within CCNA_02222 at 2371.794 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 2,369,646 - CCNA_02221 0.64 +0.3 2,369,671 - CCNA_02221 0.65 -0.4 2,369,696 + CCNA_02221 0.66 -0.1 2,369,704 - CCNA_02221 0.66 +0.4 2,369,709 - CCNA_02221 0.66 +0.5 2,369,730 + CCNA_02221 0.67 -0.2 2,369,730 + CCNA_02221 0.67 +0.1 2,369,743 + CCNA_02221 0.67 +0.8 2,369,743 + CCNA_02221 0.67 -0.2 2,369,743 + CCNA_02221 0.67 +0.3 2,369,756 - CCNA_02221 0.68 +0.5 2,369,766 + CCNA_02221 0.68 -0.7 2,369,939 - CCNA_02221 0.75 -0.8 2,370,038 - CCNA_02221 0.78 +0.9 2,370,090 + CCNA_02221 0.80 -2.0 2,370,123 + CCNA_02221 0.81 -1.7 2,370,156 + CCNA_02221 0.83 -0.0 2,370,164 + CCNA_02221 0.83 -0.7 2,370,164 + CCNA_02221 0.83 +0.1 2,370,172 - CCNA_02221 0.83 -0.2 2,370,212 + CCNA_02221 0.85 -1.6 2,370,213 + CCNA_02221 0.85 -0.6 2,370,213 + CCNA_02221 0.85 +1.3 2,370,229 - CCNA_02221 0.85 -0.1 2,370,229 - CCNA_02221 0.85 -0.2 2,370,257 - CCNA_02221 0.86 -0.8 2,370,291 - CCNA_02221 0.88 +0.9 2,370,322 - CCNA_02221 0.89 +0.1 2,370,326 + CCNA_02221 0.89 -1.4 2,370,326 + CCNA_02221 0.89 +1.7 2,370,334 - CCNA_02221 0.89 -0.1 2,370,334 - CCNA_02221 0.89 +0.5 2,370,336 + CCNA_02221 0.89 -1.3 2,370,336 + CCNA_02221 0.89 +0.3 2,370,486 + -0.4 2,370,486 + -0.5 2,370,494 - +0.4 2,370,570 + -0.3 2,370,593 + +0.6 2,370,706 + CCNA_03963 0.50 -1.8 2,370,740 - CCNA_03963 0.68 +1.4 2,370,747 - CCNA_03963 0.71 +0.8 2,370,766 + CCNA_03963 0.81 +0.3 2,370,808 + -0.8 2,370,840 + +1.6 2,370,840 + -1.2 2,370,840 + -1.0 2,370,840 + -0.2 2,370,840 + +0.7 2,370,848 - -0.2 2,370,848 - -0.3 2,370,848 - -1.1 2,370,848 - +0.1 2,370,848 - -0.1 2,370,848 - -0.4 2,370,848 - +0.3 2,370,907 + -1.2 2,370,908 + CCNA_02222 0.10 +1.0 2,370,908 + CCNA_02222 0.10 -0.6 2,370,949 + CCNA_02222 0.14 +0.7 2,370,965 + CCNA_02222 0.15 -1.2 2,371,017 + CCNA_02222 0.20 +0.1 2,371,037 + CCNA_02222 0.22 +0.8 2,371,094 + CCNA_02222 0.27 -1.3 2,371,126 - CCNA_02222 0.30 -0.7 2,371,165 - CCNA_02222 0.34 -1.0 2,371,252 + CCNA_02222 0.42 +0.1 2,371,277 + CCNA_02222 0.44 -0.6 2,371,307 - CCNA_02222 0.47 +0.4 2,371,332 + CCNA_02222 0.49 -0.3 2,371,342 + CCNA_02222 0.50 +0.2 2,371,374 + CCNA_02222 0.53 +0.5 2,371,374 + CCNA_02222 0.53 -3.7 2,371,374 + CCNA_02222 0.53 -0.3 2,371,374 + CCNA_02222 0.53 -1.3 2,371,382 - CCNA_02222 0.54 -0.5 2,371,467 + CCNA_02222 0.62 -2.0 2,371,528 + CCNA_02222 0.68 -1.4 2,371,573 + CCNA_02222 0.72 -0.6 2,371,596 - CCNA_02222 0.74 +0.8 2,371,619 - CCNA_02222 0.76 -0.4 2,371,794 - +1.1
Or see this region's nucleotide sequence