Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02019 and CCNA_02020 are separated by 82 nucleotides CCNA_02020 and CCNA_03961 are separated by 10 nucleotides CCNA_03961 and CCNA_02021 overlap by 4 nucleotides CCNA_02021 and CCNA_02022 overlap by 1 nucleotides
CCNA_02019: CCNA_02019 - NADH-quinone oxidoreductase chain J, at 2,166,636 to 2,167,253
_02019
CCNA_02020: CCNA_02020 - NADH-quinone oxidoreductase chain I, at 2,167,336 to 2,167,827
_02020
CCNA_03961: CCNA_03961 - hypothetical protein, at 2,167,838 to 2,168,062
_03961
CCNA_02021: CCNA_02021 - hypothetical protein, at 2,168,059 to 2,168,298
_02021
CCNA_02022: CCNA_02022 - NADH-quinone oxidoreductase chain H, at 2,168,298 to 2,169,368
_02022
Position (kb)
2167
2168
2169 Strain fitness (log2 ratio)
-1
0
1 at 2167.877 kb on - strand, within CCNA_03961 at 2167.877 kb on - strand, within CCNA_03961 at 2167.946 kb on - strand, within CCNA_03961 at 2167.946 kb on - strand, within CCNA_03961 at 2167.946 kb on - strand, within CCNA_03961 at 2167.959 kb on - strand, within CCNA_03961 at 2168.050 kb on - strand at 2168.063 kb on + strand at 2168.071 kb on - strand at 2168.108 kb on + strand, within CCNA_02021 at 2168.111 kb on - strand, within CCNA_02021 at 2168.114 kb on + strand, within CCNA_02021 at 2168.122 kb on - strand, within CCNA_02021 at 2168.137 kb on - strand, within CCNA_02021 at 2168.150 kb on + strand, within CCNA_02021 at 2168.150 kb on - strand, within CCNA_02021 at 2168.158 kb on - strand, within CCNA_02021 at 2168.158 kb on - strand, within CCNA_02021 at 2168.218 kb on - strand, within CCNA_02021 at 2168.288 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 2,167,877 - CCNA_03961 0.17 +1.1 2,167,877 - CCNA_03961 0.17 +0.8 2,167,946 - CCNA_03961 0.48 +1.1 2,167,946 - CCNA_03961 0.48 +0.8 2,167,946 - CCNA_03961 0.48 +0.6 2,167,959 - CCNA_03961 0.54 +1.2 2,168,050 - -1.0 2,168,063 + -1.6 2,168,071 - +0.1 2,168,108 + CCNA_02021 0.20 -0.5 2,168,111 - CCNA_02021 0.22 +1.2 2,168,114 + CCNA_02021 0.23 -0.0 2,168,122 - CCNA_02021 0.26 +0.0 2,168,137 - CCNA_02021 0.33 +0.4 2,168,150 + CCNA_02021 0.38 +1.0 2,168,150 - CCNA_02021 0.38 +1.2 2,168,158 - CCNA_02021 0.41 +0.4 2,168,158 - CCNA_02021 0.41 +0.4 2,168,218 - CCNA_02021 0.66 +0.3 2,168,288 - -0.0
Or see this region's nucleotide sequence