Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01538 and CCNA_03952 are separated by 116 nucleotides CCNA_03952 and CCNA_01539 overlap by 47 nucleotides CCNA_01539 and CCNA_01541 are separated by 125 nucleotides
CCNA_01538: CCNA_01538 - pyruvate phosphate dikinase, at 1,646,212 to 1,648,902
_01538
CCNA_03952: CCNA_03952 - hypothetical protein, at 1,649,019 to 1,649,504
_03952
CCNA_01539: CCNA_01539 - pirin family protein, at 1,649,458 to 1,650,156
_01539
CCNA_01541: CCNA_01541 - LysR-family transcriptional regulator, at 1,650,282 to 1,651,178
_01541
Position (kb)
1649
1650 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1648.509 kb on + strand, within CCNA_01538 at 1648.604 kb on + strand, within CCNA_01538 at 1648.681 kb on + strand at 1648.933 kb on + strand at 1648.941 kb on - strand at 1648.943 kb on + strand at 1649.117 kb on + strand, within CCNA_03952 at 1649.131 kb on + strand, within CCNA_03952 at 1649.221 kb on - strand, within CCNA_03952 at 1649.232 kb on + strand, within CCNA_03952 at 1649.256 kb on - strand, within CCNA_03952 at 1649.280 kb on - strand, within CCNA_03952 at 1649.282 kb on + strand, within CCNA_03952 at 1649.290 kb on - strand, within CCNA_03952 at 1649.290 kb on - strand, within CCNA_03952 at 1649.293 kb on + strand, within CCNA_03952 at 1649.367 kb on + strand, within CCNA_03952 at 1649.367 kb on + strand, within CCNA_03952 at 1649.382 kb on + strand, within CCNA_03952 at 1649.390 kb on + strand, within CCNA_03952 at 1649.390 kb on + strand, within CCNA_03952 at 1649.390 kb on + strand, within CCNA_03952 at 1649.390 kb on + strand, within CCNA_03952 at 1649.429 kb on + strand, within CCNA_03952 at 1649.446 kb on + strand, within CCNA_03952 at 1649.446 kb on + strand, within CCNA_03952 at 1649.455 kb on + strand, within CCNA_03952 at 1649.463 kb on - strand at 1649.463 kb on - strand at 1649.486 kb on + strand at 1649.501 kb on + strand at 1649.561 kb on + strand, within CCNA_01539 at 1649.569 kb on - strand, within CCNA_01539 at 1649.617 kb on - strand, within CCNA_01539 at 1649.650 kb on - strand, within CCNA_01539 at 1649.716 kb on + strand, within CCNA_01539 at 1649.747 kb on + strand, within CCNA_01539 at 1649.755 kb on - strand, within CCNA_01539 at 1649.760 kb on + strand, within CCNA_01539 at 1649.768 kb on - strand, within CCNA_01539 at 1649.804 kb on + strand, within CCNA_01539 at 1649.804 kb on + strand, within CCNA_01539 at 1649.840 kb on + strand, within CCNA_01539 at 1649.840 kb on + strand, within CCNA_01539 at 1649.848 kb on - strand, within CCNA_01539 at 1649.852 kb on + strand, within CCNA_01539 at 1649.891 kb on + strand, within CCNA_01539 at 1649.911 kb on - strand, within CCNA_01539 at 1649.978 kb on - strand, within CCNA_01539 at 1650.031 kb on + strand, within CCNA_01539 at 1650.092 kb on + strand at 1650.104 kb on + strand at 1650.112 kb on - strand at 1650.217 kb on - strand at 1650.371 kb on - strand at 1650.371 kb on - strand at 1650.406 kb on + strand, within CCNA_01541 at 1650.414 kb on - strand, within CCNA_01541 at 1650.414 kb on - strand, within CCNA_01541 at 1650.424 kb on + strand, within CCNA_01541 at 1650.432 kb on - strand, within CCNA_01541 at 1650.432 kb on - strand, within CCNA_01541
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 1,648,509 + CCNA_01538 0.85 +0.1 1,648,604 + CCNA_01538 0.89 -1.2 1,648,681 + +0.5 1,648,933 + -0.1 1,648,941 - +2.1 1,648,943 + +1.3 1,649,117 + CCNA_03952 0.20 +0.7 1,649,131 + CCNA_03952 0.23 +0.4 1,649,221 - CCNA_03952 0.42 +0.1 1,649,232 + CCNA_03952 0.44 +0.3 1,649,256 - CCNA_03952 0.49 +0.5 1,649,280 - CCNA_03952 0.54 -0.3 1,649,282 + CCNA_03952 0.54 +1.7 1,649,290 - CCNA_03952 0.56 +1.5 1,649,290 - CCNA_03952 0.56 -0.7 1,649,293 + CCNA_03952 0.56 +0.6 1,649,367 + CCNA_03952 0.72 +0.2 1,649,367 + CCNA_03952 0.72 -0.3 1,649,382 + CCNA_03952 0.75 +0.3 1,649,390 + CCNA_03952 0.76 +0.2 1,649,390 + CCNA_03952 0.76 +0.4 1,649,390 + CCNA_03952 0.76 -0.3 1,649,390 + CCNA_03952 0.76 +0.9 1,649,429 + CCNA_03952 0.84 +0.7 1,649,446 + CCNA_03952 0.88 +0.9 1,649,446 + CCNA_03952 0.88 +0.6 1,649,455 + CCNA_03952 0.90 -0.1 1,649,463 - +0.9 1,649,463 - +0.0 1,649,486 + -0.1 1,649,501 + -0.1 1,649,561 + CCNA_01539 0.15 +0.6 1,649,569 - CCNA_01539 0.16 +0.4 1,649,617 - CCNA_01539 0.23 +0.2 1,649,650 - CCNA_01539 0.27 +1.7 1,649,716 + CCNA_01539 0.37 -0.2 1,649,747 + CCNA_01539 0.41 -1.1 1,649,755 - CCNA_01539 0.42 -1.4 1,649,760 + CCNA_01539 0.43 -1.3 1,649,768 - CCNA_01539 0.44 -0.0 1,649,804 + CCNA_01539 0.49 +0.2 1,649,804 + CCNA_01539 0.49 +0.9 1,649,840 + CCNA_01539 0.55 +0.7 1,649,840 + CCNA_01539 0.55 +0.1 1,649,848 - CCNA_01539 0.56 -2.3 1,649,852 + CCNA_01539 0.56 -1.0 1,649,891 + CCNA_01539 0.62 -0.6 1,649,911 - CCNA_01539 0.65 -0.0 1,649,978 - CCNA_01539 0.74 +0.0 1,650,031 + CCNA_01539 0.82 +0.4 1,650,092 + -0.6 1,650,104 + -0.1 1,650,112 - +0.8 1,650,217 - +0.4 1,650,371 - -0.8 1,650,371 - -2.2 1,650,406 + CCNA_01541 0.14 +0.7 1,650,414 - CCNA_01541 0.15 -0.1 1,650,414 - CCNA_01541 0.15 -1.1 1,650,424 + CCNA_01541 0.16 +0.1 1,650,432 - CCNA_01541 0.17 -0.7 1,650,432 - CCNA_01541 0.17 -1.6
Or see this region's nucleotide sequence