Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01717 and CCNA_01718 overlap by 1 nucleotides CCNA_01718 and CCNA_01719 overlap by 4 nucleotides CCNA_01719 and CCNA_03893 are separated by 103 nucleotides CCNA_03893 and CCNA_01721 are separated by 22 nucleotides CCNA_01721 and CCNA_01722 overlap by 32 nucleotides
CCNA_01717: CCNA_01717 - hypothetical protein, at 1,846,800 to 1,847,210
_01717
CCNA_01718: CCNA_01718 - RNA polymerase ECF sigma factor, at 1,847,210 to 1,847,725
_01718
CCNA_01719: CCNA_01719 - hypothetical protein, at 1,847,722 to 1,848,030
_01719
CCNA_03893: CCNA_03893 - hypothetical protein, at 1,848,134 to 1,848,526
_03893
CCNA_01721: CCNA_01721 - RNA polymerase ECF sigma factor, at 1,848,549 to 1,849,133
_01721
CCNA_01722: CCNA_01722 - hypothetical protein, at 1,849,102 to 1,849,506
_01722
Position (kb)
1848
1849 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1847.153 kb on + strand, within CCNA_01717 at 1847.159 kb on - strand, within CCNA_01717 at 1847.204 kb on + strand at 1847.209 kb on + strand at 1847.209 kb on + strand at 1847.232 kb on - strand at 1847.232 kb on - strand at 1847.273 kb on - strand, within CCNA_01718 at 1847.367 kb on - strand, within CCNA_01718 at 1847.410 kb on + strand, within CCNA_01718 at 1847.439 kb on + strand, within CCNA_01718 at 1847.594 kb on + strand, within CCNA_01718 at 1847.595 kb on + strand, within CCNA_01718 at 1847.601 kb on + strand, within CCNA_01718 at 1847.601 kb on + strand, within CCNA_01718 at 1847.601 kb on + strand, within CCNA_01718 at 1847.601 kb on + strand, within CCNA_01718 at 1847.601 kb on + strand, within CCNA_01718 at 1847.606 kb on + strand, within CCNA_01718 at 1847.614 kb on - strand, within CCNA_01718 at 1847.619 kb on - strand, within CCNA_01718 at 1847.625 kb on - strand, within CCNA_01718 at 1847.651 kb on + strand, within CCNA_01718 at 1847.651 kb on + strand, within CCNA_01718 at 1847.651 kb on + strand, within CCNA_01718 at 1847.651 kb on + strand, within CCNA_01718 at 1847.651 kb on + strand, within CCNA_01718 at 1847.651 kb on + strand, within CCNA_01718 at 1847.651 kb on + strand, within CCNA_01718 at 1847.659 kb on - strand, within CCNA_01718 at 1847.659 kb on - strand, within CCNA_01718 at 1847.659 kb on - strand, within CCNA_01718 at 1847.659 kb on - strand, within CCNA_01718 at 1847.659 kb on - strand, within CCNA_01718 at 1847.659 kb on - strand, within CCNA_01718 at 1847.659 kb on - strand, within CCNA_01718 at 1847.659 kb on - strand, within CCNA_01718 at 1847.661 kb on - strand, within CCNA_01718 at 1847.705 kb on + strand at 1847.810 kb on + strand, within CCNA_01719 at 1848.006 kb on - strand at 1848.008 kb on + strand at 1848.083 kb on + strand at 1848.083 kb on + strand at 1848.091 kb on - strand at 1848.102 kb on + strand at 1848.181 kb on + strand, within CCNA_03893 at 1848.181 kb on + strand, within CCNA_03893 at 1848.189 kb on - strand, within CCNA_03893 at 1848.195 kb on + strand, within CCNA_03893 at 1848.282 kb on + strand, within CCNA_03893 at 1848.282 kb on + strand, within CCNA_03893 at 1848.363 kb on + strand, within CCNA_03893 at 1848.537 kb on - strand at 1848.590 kb on - strand at 1848.658 kb on + strand, within CCNA_01721 at 1848.666 kb on + strand, within CCNA_01721 at 1848.666 kb on + strand, within CCNA_01721 at 1848.686 kb on + strand, within CCNA_01721 at 1848.762 kb on - strand, within CCNA_01721 at 1848.812 kb on + strand, within CCNA_01721 at 1848.870 kb on - strand, within CCNA_01721 at 1848.889 kb on - strand, within CCNA_01721 at 1849.028 kb on - strand, within CCNA_01721 at 1849.085 kb on - strand at 1849.108 kb on - strand at 1849.122 kb on + strand at 1849.122 kb on + strand at 1849.122 kb on + strand at 1849.195 kb on + strand, within CCNA_01722 at 1849.200 kb on + strand, within CCNA_01722 at 1849.212 kb on + strand, within CCNA_01722 at 1849.228 kb on + strand, within CCNA_01722 at 1849.277 kb on + strand, within CCNA_01722 at 1849.282 kb on + strand, within CCNA_01722 at 1849.282 kb on + strand, within CCNA_01722 at 1849.285 kb on - strand, within CCNA_01722 at 1849.287 kb on + strand, within CCNA_01722 at 1849.297 kb on + strand, within CCNA_01722 at 1849.405 kb on + strand, within CCNA_01722 at 1849.407 kb on + strand, within CCNA_01722 at 1849.419 kb on + strand, within CCNA_01722 at 1849.419 kb on + strand, within CCNA_01722 at 1849.465 kb on + strand, within CCNA_01722 at 1849.481 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 1,847,153 + CCNA_01717 0.86 +0.3 1,847,159 - CCNA_01717 0.87 +0.7 1,847,204 + -2.3 1,847,209 + -0.9 1,847,209 + +0.7 1,847,232 - -0.1 1,847,232 - +0.3 1,847,273 - CCNA_01718 0.12 -0.1 1,847,367 - CCNA_01718 0.30 -0.6 1,847,410 + CCNA_01718 0.39 -0.3 1,847,439 + CCNA_01718 0.44 +0.0 1,847,594 + CCNA_01718 0.74 +0.3 1,847,595 + CCNA_01718 0.75 +0.4 1,847,601 + CCNA_01718 0.76 +0.2 1,847,601 + CCNA_01718 0.76 -0.2 1,847,601 + CCNA_01718 0.76 +0.0 1,847,601 + CCNA_01718 0.76 +2.0 1,847,601 + CCNA_01718 0.76 -0.4 1,847,606 + CCNA_01718 0.77 +0.1 1,847,614 - CCNA_01718 0.78 -0.1 1,847,619 - CCNA_01718 0.79 -0.3 1,847,625 - CCNA_01718 0.80 -2.6 1,847,651 + CCNA_01718 0.85 -1.0 1,847,651 + CCNA_01718 0.85 -0.2 1,847,651 + CCNA_01718 0.85 -0.7 1,847,651 + CCNA_01718 0.85 -0.2 1,847,651 + CCNA_01718 0.85 +0.2 1,847,651 + CCNA_01718 0.85 +0.5 1,847,651 + CCNA_01718 0.85 -0.3 1,847,659 - CCNA_01718 0.87 +0.2 1,847,659 - CCNA_01718 0.87 -0.8 1,847,659 - CCNA_01718 0.87 +0.7 1,847,659 - CCNA_01718 0.87 +1.1 1,847,659 - CCNA_01718 0.87 +0.4 1,847,659 - CCNA_01718 0.87 -1.1 1,847,659 - CCNA_01718 0.87 -0.3 1,847,659 - CCNA_01718 0.87 -0.8 1,847,661 - CCNA_01718 0.87 -1.9 1,847,705 + +0.2 1,847,810 + CCNA_01719 0.28 +0.1 1,848,006 - +0.3 1,848,008 + +0.7 1,848,083 + +0.8 1,848,083 + +0.3 1,848,091 - -1.7 1,848,102 + +0.4 1,848,181 + CCNA_03893 0.12 +0.6 1,848,181 + CCNA_03893 0.12 +0.9 1,848,189 - CCNA_03893 0.14 +0.7 1,848,195 + CCNA_03893 0.16 +1.0 1,848,282 + CCNA_03893 0.38 -0.3 1,848,282 + CCNA_03893 0.38 +1.2 1,848,363 + CCNA_03893 0.58 -0.5 1,848,537 - +0.1 1,848,590 - +0.2 1,848,658 + CCNA_01721 0.19 +0.9 1,848,666 + CCNA_01721 0.20 +1.1 1,848,666 + CCNA_01721 0.20 +1.3 1,848,686 + CCNA_01721 0.23 +1.3 1,848,762 - CCNA_01721 0.36 -1.6 1,848,812 + CCNA_01721 0.45 +0.5 1,848,870 - CCNA_01721 0.55 -1.0 1,848,889 - CCNA_01721 0.58 +0.7 1,849,028 - CCNA_01721 0.82 -0.3 1,849,085 - +0.2 1,849,108 - -0.2 1,849,122 + +0.2 1,849,122 + +2.7 1,849,122 + -0.0 1,849,195 + CCNA_01722 0.23 +0.5 1,849,200 + CCNA_01722 0.24 -0.8 1,849,212 + CCNA_01722 0.27 +0.7 1,849,228 + CCNA_01722 0.31 +0.7 1,849,277 + CCNA_01722 0.43 -0.7 1,849,282 + CCNA_01722 0.44 +0.6 1,849,282 + CCNA_01722 0.44 +0.0 1,849,285 - CCNA_01722 0.45 -1.6 1,849,287 + CCNA_01722 0.46 +0.5 1,849,297 + CCNA_01722 0.48 +1.6 1,849,405 + CCNA_01722 0.75 -0.1 1,849,407 + CCNA_01722 0.75 -0.2 1,849,419 + CCNA_01722 0.78 +0.2 1,849,419 + CCNA_01722 0.78 -0.7 1,849,465 + CCNA_01722 0.90 +0.7 1,849,481 + -0.2
Or see this region's nucleotide sequence