Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_03764 and CCNA_03765 are separated by 3 nucleotides CCNA_03765 and CCNA_03766 are separated by 23 nucleotides CCNA_03766 and CCNA_03767 are separated by 56 nucleotides CCNA_03767 and CCNA_03768 are separated by 26 nucleotides
CCNA_03764: CCNA_03764 - acyl-CoA thioesterase, at 3,932,758 to 3,933,612
_03764
CCNA_03765: CCNA_03765 - phosphohydrolase, MutT/nudix family protein, at 3,933,616 to 3,934,179
_03765
CCNA_03766: CCNA_03766 - 16S rRNA processing protein RimM, at 3,934,203 to 3,934,784
_03766
CCNA_03767: CCNA_03767 - SSU ribosomal protein S16P, at 3,934,841 to 3,935,338
_03767
CCNA_03768: CCNA_03768 - signal recognition particle GTPase, SRP, at 3,935,365 to 3,936,891
_03768
Position (kb)
3934
3935 Strain fitness (log2 ratio)
-1
0
1
2 at 3933.257 kb on - strand, within CCNA_03764 at 3933.281 kb on - strand, within CCNA_03764 at 3933.288 kb on + strand, within CCNA_03764 at 3933.289 kb on + strand, within CCNA_03764 at 3933.297 kb on - strand, within CCNA_03764 at 3933.310 kb on + strand, within CCNA_03764 at 3933.343 kb on + strand, within CCNA_03764 at 3933.348 kb on + strand, within CCNA_03764 at 3933.351 kb on - strand, within CCNA_03764 at 3933.410 kb on - strand, within CCNA_03764 at 3933.410 kb on - strand, within CCNA_03764 at 3933.415 kb on - strand, within CCNA_03764 at 3933.433 kb on + strand, within CCNA_03764 at 3933.434 kb on + strand, within CCNA_03764 at 3933.446 kb on - strand, within CCNA_03764 at 3933.446 kb on - strand, within CCNA_03764 at 3933.493 kb on + strand, within CCNA_03764 at 3933.501 kb on - strand, within CCNA_03764 at 3933.501 kb on - strand, within CCNA_03764 at 3933.501 kb on - strand, within CCNA_03764 at 3933.501 kb on - strand, within CCNA_03764 at 3933.509 kb on + strand, within CCNA_03764 at 3933.564 kb on + strand at 3933.621 kb on - strand at 3933.621 kb on - strand at 3933.622 kb on - strand at 3933.700 kb on + strand, within CCNA_03765 at 3933.708 kb on - strand, within CCNA_03765 at 3933.708 kb on - strand, within CCNA_03765 at 3933.743 kb on + strand, within CCNA_03765 at 3933.751 kb on - strand, within CCNA_03765 at 3933.751 kb on - strand, within CCNA_03765 at 3933.751 kb on - strand, within CCNA_03765 at 3933.751 kb on - strand, within CCNA_03765 at 3933.753 kb on + strand, within CCNA_03765 at 3933.756 kb on + strand, within CCNA_03765 at 3933.775 kb on - strand, within CCNA_03765 at 3933.811 kb on - strand, within CCNA_03765 at 3933.844 kb on - strand, within CCNA_03765 at 3933.861 kb on + strand, within CCNA_03765 at 3933.869 kb on - strand, within CCNA_03765 at 3933.929 kb on - strand, within CCNA_03765 at 3933.980 kb on - strand, within CCNA_03765 at 3933.980 kb on - strand, within CCNA_03765 at 3933.991 kb on - strand, within CCNA_03765 at 3933.995 kb on + strand, within CCNA_03765 at 3934.045 kb on + strand, within CCNA_03765 at 3934.048 kb on - strand, within CCNA_03765 at 3934.054 kb on + strand, within CCNA_03765 at 3934.061 kb on + strand, within CCNA_03765 at 3934.094 kb on + strand, within CCNA_03765 at 3934.124 kb on - strand at 3934.129 kb on + strand at 3934.293 kb on + strand, within CCNA_03766 at 3934.293 kb on + strand, within CCNA_03766 at 3934.301 kb on - strand, within CCNA_03766 at 3934.798 kb on - strand at 3935.015 kb on - strand, within CCNA_03767 at 3935.015 kb on - strand, within CCNA_03767 at 3935.419 kb on - strand at 3935.468 kb on - strand at 3935.478 kb on - strand at 3935.506 kb on - strand at 3935.557 kb on - strand, within CCNA_03768 at 3935.611 kb on - strand, within CCNA_03768 at 3935.611 kb on - strand, within CCNA_03768 at 3935.611 kb on - strand, within CCNA_03768
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 3,933,257 - CCNA_03764 0.58 +0.3 3,933,281 - CCNA_03764 0.61 +0.5 3,933,288 + CCNA_03764 0.62 +0.4 3,933,289 + CCNA_03764 0.62 -1.2 3,933,297 - CCNA_03764 0.63 -0.1 3,933,310 + CCNA_03764 0.65 +0.5 3,933,343 + CCNA_03764 0.68 -0.1 3,933,348 + CCNA_03764 0.69 +0.8 3,933,351 - CCNA_03764 0.69 -0.3 3,933,410 - CCNA_03764 0.76 +1.0 3,933,410 - CCNA_03764 0.76 -1.5 3,933,415 - CCNA_03764 0.77 +0.5 3,933,433 + CCNA_03764 0.79 +0.4 3,933,434 + CCNA_03764 0.79 -0.3 3,933,446 - CCNA_03764 0.80 -1.3 3,933,446 - CCNA_03764 0.80 +0.3 3,933,493 + CCNA_03764 0.86 +0.6 3,933,501 - CCNA_03764 0.87 -0.3 3,933,501 - CCNA_03764 0.87 +0.3 3,933,501 - CCNA_03764 0.87 -0.0 3,933,501 - CCNA_03764 0.87 +0.5 3,933,509 + CCNA_03764 0.88 -0.8 3,933,564 + -0.7 3,933,621 - -1.1 3,933,621 - +0.2 3,933,622 - +0.3 3,933,700 + CCNA_03765 0.15 -0.8 3,933,708 - CCNA_03765 0.16 -0.0 3,933,708 - CCNA_03765 0.16 -0.2 3,933,743 + CCNA_03765 0.23 -0.2 3,933,751 - CCNA_03765 0.24 +0.1 3,933,751 - CCNA_03765 0.24 -0.7 3,933,751 - CCNA_03765 0.24 -0.4 3,933,751 - CCNA_03765 0.24 +0.9 3,933,753 + CCNA_03765 0.24 +0.2 3,933,756 + CCNA_03765 0.25 +0.6 3,933,775 - CCNA_03765 0.28 +0.0 3,933,811 - CCNA_03765 0.35 +0.4 3,933,844 - CCNA_03765 0.40 -0.7 3,933,861 + CCNA_03765 0.43 +0.9 3,933,869 - CCNA_03765 0.45 -1.0 3,933,929 - CCNA_03765 0.55 +0.4 3,933,980 - CCNA_03765 0.65 +0.6 3,933,980 - CCNA_03765 0.65 +0.8 3,933,991 - CCNA_03765 0.66 +0.5 3,933,995 + CCNA_03765 0.67 -1.6 3,934,045 + CCNA_03765 0.76 +0.0 3,934,048 - CCNA_03765 0.77 +0.7 3,934,054 + CCNA_03765 0.78 -0.0 3,934,061 + CCNA_03765 0.79 -0.8 3,934,094 + CCNA_03765 0.85 +0.2 3,934,124 - -0.4 3,934,129 + -1.4 3,934,293 + CCNA_03766 0.15 +0.5 3,934,293 + CCNA_03766 0.15 +0.3 3,934,301 - CCNA_03766 0.17 +1.2 3,934,798 - +0.3 3,935,015 - CCNA_03767 0.35 +2.0 3,935,015 - CCNA_03767 0.35 -0.0 3,935,419 - -0.8 3,935,468 - -0.7 3,935,478 - +0.3 3,935,506 - +1.1 3,935,557 - CCNA_03768 0.13 -0.3 3,935,611 - CCNA_03768 0.16 -0.9 3,935,611 - CCNA_03768 0.16 -0.1 3,935,611 - CCNA_03768 0.16 +0.5
Or see this region's nucleotide sequence