Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_03699 and CCNA_03700 are separated by 78 nucleotides CCNA_03700 and CCNA_03701 are separated by 81 nucleotides CCNA_03701 and CCNA_03702 are separated by 202 nucleotides
CCNA_03699: CCNA_03699 - peptidase, M16 family, at 3,865,772 to 3,868,618
_03699
CCNA_03700: CCNA_03700 - large-conductance mechanosensitive channel, at 3,868,697 to 3,869,116
_03700
CCNA_03701: CCNA_03701 - integration host factor beta-subunit, at 3,869,198 to 3,869,488
_03701
CCNA_03702: CCNA_03702 - SSU ribosomal protein S1P, at 3,869,691 to 3,871,400
_03702
Position (kb)
3869
3870 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3868.241 kb on + strand, within CCNA_03699 at 3868.245 kb on - strand, within CCNA_03699 at 3868.249 kb on - strand, within CCNA_03699 at 3868.250 kb on - strand, within CCNA_03699 at 3868.256 kb on + strand, within CCNA_03699 at 3868.256 kb on + strand, within CCNA_03699 at 3868.264 kb on - strand, within CCNA_03699 at 3868.264 kb on - strand, within CCNA_03699 at 3868.291 kb on - strand, within CCNA_03699 at 3868.350 kb on - strand at 3868.373 kb on - strand at 3868.412 kb on + strand at 3868.412 kb on + strand at 3868.420 kb on - strand at 3868.432 kb on + strand at 3868.440 kb on - strand at 3868.440 kb on - strand at 3868.456 kb on + strand at 3868.456 kb on + strand at 3868.456 kb on + strand at 3868.457 kb on + strand at 3868.457 kb on + strand at 3868.464 kb on - strand at 3868.464 kb on - strand at 3868.464 kb on - strand at 3868.464 kb on - strand at 3868.465 kb on - strand at 3868.465 kb on - strand at 3868.474 kb on + strand at 3868.482 kb on - strand at 3868.490 kb on + strand at 3868.525 kb on + strand at 3868.531 kb on + strand at 3868.532 kb on + strand at 3868.539 kb on - strand at 3868.543 kb on + strand at 3868.543 kb on + strand at 3868.543 kb on + strand at 3868.543 kb on + strand at 3868.543 kb on + strand at 3868.543 kb on + strand at 3868.546 kb on - strand at 3868.551 kb on - strand at 3868.551 kb on - strand at 3868.551 kb on - strand at 3868.551 kb on - strand at 3868.599 kb on + strand at 3868.613 kb on - strand at 3868.613 kb on - strand at 3868.703 kb on - strand at 3868.814 kb on - strand, within CCNA_03700 at 3868.876 kb on - strand, within CCNA_03700 at 3868.946 kb on - strand, within CCNA_03700 at 3869.011 kb on + strand, within CCNA_03700 at 3869.015 kb on + strand, within CCNA_03700 at 3869.109 kb on + strand at 3869.135 kb on + strand at 3869.135 kb on + strand at 3869.136 kb on - strand at 3869.136 kb on - strand at 3869.136 kb on - strand at 3869.143 kb on - strand at 3869.168 kb on - strand at 3869.382 kb on - strand, within CCNA_03701 at 3869.503 kb on - strand at 3869.533 kb on + strand at 3869.564 kb on - strand at 3869.615 kb on - strand at 3869.657 kb on - strand at 3869.697 kb on - strand at 3869.704 kb on - strand at 3869.900 kb on + strand, within CCNA_03702 at 3870.236 kb on + strand, within CCNA_03702 at 3870.329 kb on + strand, within CCNA_03702 at 3870.337 kb on - strand, within CCNA_03702
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 3,868,241 + CCNA_03699 0.87 +0.0 3,868,245 - CCNA_03699 0.87 -0.1 3,868,249 - CCNA_03699 0.87 -0.3 3,868,250 - CCNA_03699 0.87 +0.5 3,868,256 + CCNA_03699 0.87 +0.1 3,868,256 + CCNA_03699 0.87 -0.4 3,868,264 - CCNA_03699 0.88 -0.1 3,868,264 - CCNA_03699 0.88 +0.2 3,868,291 - CCNA_03699 0.88 +0.5 3,868,350 - +0.3 3,868,373 - -0.7 3,868,412 + +0.2 3,868,412 + +0.1 3,868,420 - -1.5 3,868,432 + +1.3 3,868,440 - +0.1 3,868,440 - +0.1 3,868,456 + +0.4 3,868,456 + -1.0 3,868,456 + -0.7 3,868,457 + +0.3 3,868,457 + +0.5 3,868,464 - +0.2 3,868,464 - +0.5 3,868,464 - -0.2 3,868,464 - -0.2 3,868,465 - +0.2 3,868,465 - +0.8 3,868,474 + +0.1 3,868,482 - -0.8 3,868,490 + -0.1 3,868,525 + +0.3 3,868,531 + +0.3 3,868,532 + -1.3 3,868,539 - -0.9 3,868,543 + +0.3 3,868,543 + +0.0 3,868,543 + -1.0 3,868,543 + +0.0 3,868,543 + -0.2 3,868,543 + +1.8 3,868,546 - +0.2 3,868,551 - -0.1 3,868,551 - +0.4 3,868,551 - -0.6 3,868,551 - -0.3 3,868,599 + +0.4 3,868,613 - +0.3 3,868,613 - -0.6 3,868,703 - +0.0 3,868,814 - CCNA_03700 0.28 +0.3 3,868,876 - CCNA_03700 0.43 -0.2 3,868,946 - CCNA_03700 0.59 -0.3 3,869,011 + CCNA_03700 0.75 -1.8 3,869,015 + CCNA_03700 0.76 +1.2 3,869,109 + -0.6 3,869,135 + -0.2 3,869,135 + +0.4 3,869,136 - +0.3 3,869,136 - +0.8 3,869,136 - -2.1 3,869,143 - +0.1 3,869,168 - -0.9 3,869,382 - CCNA_03701 0.63 -1.0 3,869,503 - -0.4 3,869,533 + -0.2 3,869,564 - +2.4 3,869,615 - +0.2 3,869,657 - +0.5 3,869,697 - +0.1 3,869,704 - +0.0 3,869,900 + CCNA_03702 0.12 -0.1 3,870,236 + CCNA_03702 0.32 +1.8 3,870,329 + CCNA_03702 0.37 -1.5 3,870,337 - CCNA_03702 0.38 -2.7
Or see this region's nucleotide sequence