Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_03398 and CCNA_03399 overlap by 74 nucleotides CCNA_03399 and CCNA_03400 are separated by 129 nucleotides CCNA_03400 and CCNA_03401 are separated by 80 nucleotides CCNA_03401 and CCNA_03402 are separated by 140 nucleotides
CCNA_03398: CCNA_03398 - hypothetical protein, at 3,568,899 to 3,569,450
_03398
CCNA_03399: CCNA_03399 - 3-polyprenyl-4-hydroxybenzoate decarboxylase, at 3,569,377 to 3,569,994
_03399
CCNA_03400: CCNA_03400 - hypothetical protein, at 3,570,124 to 3,570,297
_03400
CCNA_03401: CCNA_03401 - conserved hypothetical cytosolic protein, at 3,570,378 to 3,571,091
_03401
CCNA_03402: CCNA_03402 - NAD(P)H quinone oxidoreductase, PIG3 family, at 3,571,232 to 3,572,206
_03402
Position (kb)
3570
3571 Strain fitness (log2 ratio)
-2
-1
0
1 at 3569.176 kb on + strand, within CCNA_03398 at 3569.184 kb on - strand, within CCNA_03398 at 3569.188 kb on - strand, within CCNA_03398 at 3569.229 kb on + strand, within CCNA_03398 at 3569.232 kb on - strand, within CCNA_03398 at 3569.232 kb on - strand, within CCNA_03398 at 3569.237 kb on - strand, within CCNA_03398 at 3569.237 kb on - strand, within CCNA_03398 at 3569.242 kb on - strand, within CCNA_03398 at 3569.251 kb on + strand, within CCNA_03398 at 3569.251 kb on + strand, within CCNA_03398 at 3569.259 kb on - strand, within CCNA_03398 at 3569.259 kb on - strand, within CCNA_03398 at 3569.259 kb on - strand, within CCNA_03398 at 3569.259 kb on - strand, within CCNA_03398 at 3569.259 kb on - strand, within CCNA_03398 at 3569.261 kb on + strand, within CCNA_03398 at 3569.317 kb on + strand, within CCNA_03398 at 3569.354 kb on - strand, within CCNA_03398 at 3569.355 kb on - strand, within CCNA_03398 at 3569.374 kb on + strand, within CCNA_03398 at 3569.382 kb on - strand at 3569.389 kb on - strand at 3569.389 kb on - strand at 3569.433 kb on + strand at 3569.578 kb on - strand, within CCNA_03399 at 3569.639 kb on - strand, within CCNA_03399 at 3569.708 kb on + strand, within CCNA_03399 at 3569.711 kb on - strand, within CCNA_03399 at 3569.711 kb on - strand, within CCNA_03399 at 3569.716 kb on - strand, within CCNA_03399 at 3569.723 kb on + strand, within CCNA_03399 at 3569.789 kb on + strand, within CCNA_03399 at 3569.789 kb on + strand, within CCNA_03399 at 3569.842 kb on + strand, within CCNA_03399 at 3569.842 kb on + strand, within CCNA_03399 at 3569.849 kb on - strand, within CCNA_03399 at 3570.069 kb on - strand at 3570.114 kb on + strand at 3570.121 kb on + strand at 3570.129 kb on - strand at 3570.158 kb on - strand, within CCNA_03400 at 3570.223 kb on + strand, within CCNA_03400 at 3570.234 kb on + strand, within CCNA_03400 at 3570.375 kb on + strand at 3570.376 kb on + strand at 3570.383 kb on - strand at 3570.383 kb on - strand at 3570.441 kb on - strand at 3570.441 kb on - strand at 3570.452 kb on - strand, within CCNA_03401 at 3570.462 kb on - strand, within CCNA_03401 at 3570.483 kb on - strand, within CCNA_03401 at 3570.495 kb on - strand, within CCNA_03401 at 3570.529 kb on - strand, within CCNA_03401 at 3570.628 kb on - strand, within CCNA_03401 at 3570.651 kb on - strand, within CCNA_03401 at 3570.699 kb on - strand, within CCNA_03401 at 3570.790 kb on - strand, within CCNA_03401 at 3570.855 kb on + strand, within CCNA_03401 at 3570.888 kb on - strand, within CCNA_03401 at 3570.905 kb on - strand, within CCNA_03401 at 3571.080 kb on - strand at 3571.150 kb on + strand at 3571.208 kb on + strand at 3571.216 kb on - strand at 3571.243 kb on + strand at 3571.277 kb on - strand at 3571.277 kb on - strand at 3571.277 kb on - strand at 3571.288 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 3,569,176 + CCNA_03398 0.50 -0.2 3,569,184 - CCNA_03398 0.52 +0.6 3,569,188 - CCNA_03398 0.52 +1.6 3,569,229 + CCNA_03398 0.60 +1.0 3,569,232 - CCNA_03398 0.60 +0.4 3,569,232 - CCNA_03398 0.60 +0.0 3,569,237 - CCNA_03398 0.61 +0.2 3,569,237 - CCNA_03398 0.61 -0.0 3,569,242 - CCNA_03398 0.62 +0.1 3,569,251 + CCNA_03398 0.64 -0.2 3,569,251 + CCNA_03398 0.64 -0.5 3,569,259 - CCNA_03398 0.65 +0.3 3,569,259 - CCNA_03398 0.65 +0.7 3,569,259 - CCNA_03398 0.65 +0.4 3,569,259 - CCNA_03398 0.65 +0.9 3,569,259 - CCNA_03398 0.65 +0.8 3,569,261 + CCNA_03398 0.66 -0.3 3,569,317 + CCNA_03398 0.76 -0.4 3,569,354 - CCNA_03398 0.82 +1.5 3,569,355 - CCNA_03398 0.83 -0.2 3,569,374 + CCNA_03398 0.86 -0.6 3,569,382 - +0.3 3,569,389 - -2.1 3,569,389 - +1.0 3,569,433 + +0.4 3,569,578 - CCNA_03399 0.33 -0.4 3,569,639 - CCNA_03399 0.42 +0.4 3,569,708 + CCNA_03399 0.54 +0.5 3,569,711 - CCNA_03399 0.54 +0.3 3,569,711 - CCNA_03399 0.54 -0.1 3,569,716 - CCNA_03399 0.55 -0.2 3,569,723 + CCNA_03399 0.56 -1.0 3,569,789 + CCNA_03399 0.67 -0.0 3,569,789 + CCNA_03399 0.67 -1.5 3,569,842 + CCNA_03399 0.75 +0.3 3,569,842 + CCNA_03399 0.75 -1.6 3,569,849 - CCNA_03399 0.76 +0.5 3,570,069 - +0.0 3,570,114 + +0.0 3,570,121 + +0.8 3,570,129 - +0.4 3,570,158 - CCNA_03400 0.20 +0.7 3,570,223 + CCNA_03400 0.57 +0.7 3,570,234 + CCNA_03400 0.63 +0.4 3,570,375 + +0.1 3,570,376 + -0.5 3,570,383 - -0.2 3,570,383 - +0.5 3,570,441 - -0.4 3,570,441 - -0.8 3,570,452 - CCNA_03401 0.10 +0.5 3,570,462 - CCNA_03401 0.12 +0.4 3,570,483 - CCNA_03401 0.15 +0.5 3,570,495 - CCNA_03401 0.16 +0.1 3,570,529 - CCNA_03401 0.21 -0.1 3,570,628 - CCNA_03401 0.35 -0.6 3,570,651 - CCNA_03401 0.38 +0.1 3,570,699 - CCNA_03401 0.45 +0.5 3,570,790 - CCNA_03401 0.58 -1.7 3,570,855 + CCNA_03401 0.67 +0.1 3,570,888 - CCNA_03401 0.71 +0.8 3,570,905 - CCNA_03401 0.74 +1.2 3,571,080 - +0.5 3,571,150 + -0.5 3,571,208 + -0.7 3,571,216 - -0.4 3,571,243 + -1.6 3,571,277 - +0.1 3,571,277 - +0.7 3,571,277 - +0.0 3,571,288 - +0.2
Or see this region's nucleotide sequence