Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_03370 and CCNA_03371 overlap by 4 nucleotides CCNA_03371 and CCNA_03372 are separated by 152 nucleotides CCNA_03372 and CCNA_03373 are separated by 131 nucleotides CCNA_03373 and CCNA_03374 are separated by 88 nucleotides
CCNA_03370: CCNA_03370 - acetyltransferase, at 3,546,207 to 3,546,728
_03370
CCNA_03371: CCNA_03371 - bacterioferritin, at 3,546,725 to 3,547,204
_03371
CCNA_03372: CCNA_03372 - bacterioferritin-associated ferredoxin, at 3,547,357 to 3,547,542
_03372
CCNA_03373: CCNA_03373 - thiamin-phosphate pyrophosphorylase, at 3,547,674 to 3,548,318
_03373
CCNA_03374: CCNA_03374 - hypothetical protein, at 3,548,407 to 3,548,814
_03374
Position (kb)
3547
3548 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3546.430 kb on - strand, within CCNA_03370 at 3546.432 kb on + strand, within CCNA_03370 at 3546.432 kb on + strand, within CCNA_03370 at 3546.448 kb on + strand, within CCNA_03370 at 3546.466 kb on - strand, within CCNA_03370 at 3546.501 kb on + strand, within CCNA_03370 at 3546.501 kb on + strand, within CCNA_03370 at 3546.501 kb on + strand, within CCNA_03370 at 3546.509 kb on - strand, within CCNA_03370 at 3546.509 kb on - strand, within CCNA_03370 at 3546.509 kb on - strand, within CCNA_03370 at 3546.509 kb on - strand, within CCNA_03370 at 3546.509 kb on - strand, within CCNA_03370 at 3546.509 kb on - strand, within CCNA_03370 at 3546.514 kb on - strand, within CCNA_03370 at 3546.514 kb on - strand, within CCNA_03370 at 3546.543 kb on - strand, within CCNA_03370 at 3546.621 kb on - strand, within CCNA_03370 at 3546.632 kb on + strand, within CCNA_03370 at 3546.636 kb on - strand, within CCNA_03370 at 3546.664 kb on - strand, within CCNA_03370 at 3546.675 kb on + strand, within CCNA_03370 at 3546.731 kb on - strand at 3546.758 kb on + strand at 3546.772 kb on - strand at 3546.777 kb on + strand, within CCNA_03371 at 3546.809 kb on + strand, within CCNA_03371 at 3546.809 kb on + strand, within CCNA_03371 at 3546.825 kb on + strand, within CCNA_03371 at 3546.876 kb on + strand, within CCNA_03371 at 3546.894 kb on - strand, within CCNA_03371 at 3546.894 kb on - strand, within CCNA_03371 at 3546.907 kb on - strand, within CCNA_03371 at 3546.912 kb on + strand, within CCNA_03371 at 3546.912 kb on + strand, within CCNA_03371 at 3546.995 kb on - strand, within CCNA_03371 at 3547.008 kb on + strand, within CCNA_03371 at 3547.016 kb on - strand, within CCNA_03371 at 3547.031 kb on - strand, within CCNA_03371 at 3547.072 kb on + strand, within CCNA_03371 at 3547.107 kb on + strand, within CCNA_03371 at 3547.167 kb on + strand at 3547.167 kb on + strand at 3547.167 kb on + strand at 3547.167 kb on + strand at 3547.175 kb on - strand at 3547.195 kb on - strand at 3547.201 kb on + strand at 3547.272 kb on + strand at 3547.380 kb on - strand, within CCNA_03372 at 3547.620 kb on + strand at 3547.626 kb on - strand at 3547.648 kb on - strand at 3548.282 kb on - strand at 3548.298 kb on + strand at 3548.306 kb on - strand at 3548.356 kb on - strand at 3548.358 kb on + strand at 3548.366 kb on - strand at 3548.366 kb on - strand at 3548.461 kb on + strand, within CCNA_03374 at 3548.500 kb on + strand, within CCNA_03374
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 3,546,430 - CCNA_03370 0.43 -0.2 3,546,432 + CCNA_03370 0.43 -0.8 3,546,432 + CCNA_03370 0.43 +0.2 3,546,448 + CCNA_03370 0.46 -0.0 3,546,466 - CCNA_03370 0.50 +0.3 3,546,501 + CCNA_03370 0.56 +0.5 3,546,501 + CCNA_03370 0.56 +1.3 3,546,501 + CCNA_03370 0.56 -0.4 3,546,509 - CCNA_03370 0.58 +0.6 3,546,509 - CCNA_03370 0.58 -0.1 3,546,509 - CCNA_03370 0.58 -0.2 3,546,509 - CCNA_03370 0.58 +0.0 3,546,509 - CCNA_03370 0.58 +1.1 3,546,509 - CCNA_03370 0.58 +1.8 3,546,514 - CCNA_03370 0.59 +0.1 3,546,514 - CCNA_03370 0.59 +1.2 3,546,543 - CCNA_03370 0.64 +0.5 3,546,621 - CCNA_03370 0.79 +0.4 3,546,632 + CCNA_03370 0.81 +1.8 3,546,636 - CCNA_03370 0.82 +0.2 3,546,664 - CCNA_03370 0.88 +1.0 3,546,675 + CCNA_03370 0.90 -0.1 3,546,731 - +0.2 3,546,758 + -0.2 3,546,772 - +0.1 3,546,777 + CCNA_03371 0.11 +0.2 3,546,809 + CCNA_03371 0.17 -1.7 3,546,809 + CCNA_03371 0.17 -0.3 3,546,825 + CCNA_03371 0.21 -2.8 3,546,876 + CCNA_03371 0.31 +0.6 3,546,894 - CCNA_03371 0.35 +0.1 3,546,894 - CCNA_03371 0.35 +1.1 3,546,907 - CCNA_03371 0.38 +0.2 3,546,912 + CCNA_03371 0.39 -0.3 3,546,912 + CCNA_03371 0.39 -0.6 3,546,995 - CCNA_03371 0.56 +0.6 3,547,008 + CCNA_03371 0.59 -0.9 3,547,016 - CCNA_03371 0.61 -0.1 3,547,031 - CCNA_03371 0.64 +0.5 3,547,072 + CCNA_03371 0.72 -1.4 3,547,107 + CCNA_03371 0.80 -1.3 3,547,167 + -0.3 3,547,167 + -0.5 3,547,167 + -1.9 3,547,167 + -0.4 3,547,175 - +0.3 3,547,195 - +0.0 3,547,201 + -0.5 3,547,272 + -1.6 3,547,380 - CCNA_03372 0.12 +0.7 3,547,620 + +0.0 3,547,626 - -0.3 3,547,648 - -0.5 3,548,282 - +0.5 3,548,298 + +3.0 3,548,306 - +1.5 3,548,356 - -0.8 3,548,358 + -1.0 3,548,366 - -1.8 3,548,366 - +0.3 3,548,461 + CCNA_03374 0.13 -0.4 3,548,500 + CCNA_03374 0.23 -0.5
Or see this region's nucleotide sequence