Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02263 and CCNA_02264 are separated by 178 nucleotides CCNA_02264 and CCNA_02265 are separated by 52 nucleotides CCNA_02265 and CCNA_02266 are separated by 56 nucleotides
CCNA_02263: CCNA_02263 - glcG-family protein, at 2,417,355 to 2,417,846
_02263
CCNA_02264: CCNA_02264 - thiJ/PfpI family, at 2,418,025 to 2,418,600
_02264
CCNA_02265: CCNA_02265 - peptide methionine sulfoxide reductase msrB, at 2,418,653 to 2,419,132
_02265
CCNA_02266: CCNA_02266 - glutathione S-transferase GST, at 2,419,189 to 2,420,253
_02266
Position (kb)
2418
2419
2420 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2417.661 kb on + strand, within CCNA_02263 at 2417.679 kb on + strand, within CCNA_02263 at 2417.752 kb on - strand, within CCNA_02263 at 2417.799 kb on + strand at 2417.802 kb on - strand at 2417.807 kb on - strand at 2417.809 kb on + strand at 2417.809 kb on + strand at 2417.826 kb on + strand at 2417.868 kb on + strand at 2417.876 kb on - strand at 2417.923 kb on + strand at 2418.005 kb on + strand at 2418.045 kb on - strand at 2418.126 kb on - strand, within CCNA_02264 at 2418.182 kb on + strand, within CCNA_02264 at 2418.182 kb on + strand, within CCNA_02264 at 2418.205 kb on + strand, within CCNA_02264 at 2418.213 kb on - strand, within CCNA_02264 at 2418.213 kb on - strand, within CCNA_02264 at 2418.245 kb on - strand, within CCNA_02264 at 2418.325 kb on + strand, within CCNA_02264 at 2418.342 kb on + strand, within CCNA_02264 at 2418.368 kb on + strand, within CCNA_02264 at 2418.381 kb on - strand, within CCNA_02264 at 2418.415 kb on + strand, within CCNA_02264 at 2418.415 kb on + strand, within CCNA_02264 at 2418.415 kb on + strand, within CCNA_02264 at 2418.423 kb on - strand, within CCNA_02264 at 2418.423 kb on - strand, within CCNA_02264 at 2418.428 kb on - strand, within CCNA_02264 at 2418.520 kb on - strand, within CCNA_02264 at 2418.525 kb on + strand, within CCNA_02264 at 2418.535 kb on + strand, within CCNA_02264 at 2418.543 kb on - strand at 2418.543 kb on - strand at 2418.543 kb on - strand at 2418.598 kb on + strand at 2418.649 kb on + strand at 2418.670 kb on + strand at 2418.684 kb on + strand at 2418.684 kb on + strand at 2418.689 kb on + strand at 2418.692 kb on - strand at 2418.759 kb on - strand, within CCNA_02265 at 2418.856 kb on + strand, within CCNA_02265 at 2418.880 kb on - strand, within CCNA_02265 at 2418.898 kb on - strand, within CCNA_02265 at 2419.003 kb on + strand, within CCNA_02265 at 2419.004 kb on + strand, within CCNA_02265 at 2419.058 kb on - strand, within CCNA_02265 at 2419.086 kb on + strand at 2419.101 kb on + strand at 2419.116 kb on - strand at 2419.215 kb on + strand at 2419.242 kb on - strand at 2419.248 kb on - strand at 2419.264 kb on + strand at 2419.296 kb on + strand, within CCNA_02266 at 2419.324 kb on - strand, within CCNA_02266 at 2419.376 kb on - strand, within CCNA_02266 at 2419.385 kb on - strand, within CCNA_02266 at 2419.385 kb on - strand, within CCNA_02266 at 2419.435 kb on - strand, within CCNA_02266 at 2419.435 kb on - strand, within CCNA_02266 at 2419.524 kb on + strand, within CCNA_02266 at 2419.537 kb on - strand, within CCNA_02266 at 2419.537 kb on - strand, within CCNA_02266 at 2419.553 kb on - strand, within CCNA_02266 at 2419.553 kb on - strand, within CCNA_02266 at 2419.566 kb on - strand, within CCNA_02266 at 2419.595 kb on + strand, within CCNA_02266 at 2419.608 kb on + strand, within CCNA_02266 at 2419.611 kb on + strand, within CCNA_02266 at 2419.624 kb on - strand, within CCNA_02266 at 2419.633 kb on - strand, within CCNA_02266 at 2419.633 kb on - strand, within CCNA_02266 at 2419.633 kb on - strand, within CCNA_02266 at 2419.633 kb on - strand, within CCNA_02266 at 2419.635 kb on + strand, within CCNA_02266 at 2419.635 kb on + strand, within CCNA_02266 at 2419.638 kb on - strand, within CCNA_02266 at 2419.692 kb on + strand, within CCNA_02266 at 2419.692 kb on + strand, within CCNA_02266 at 2419.692 kb on + strand, within CCNA_02266 at 2419.700 kb on - strand, within CCNA_02266 at 2419.700 kb on - strand, within CCNA_02266 at 2419.700 kb on - strand, within CCNA_02266 at 2419.714 kb on - strand, within CCNA_02266 at 2419.714 kb on - strand, within CCNA_02266 at 2419.762 kb on + strand, within CCNA_02266 at 2419.762 kb on + strand, within CCNA_02266 at 2419.770 kb on - strand, within CCNA_02266 at 2419.770 kb on - strand, within CCNA_02266 at 2419.772 kb on + strand, within CCNA_02266 at 2419.790 kb on + strand, within CCNA_02266 at 2419.790 kb on + strand, within CCNA_02266 at 2419.790 kb on + strand, within CCNA_02266 at 2419.798 kb on - strand, within CCNA_02266 at 2419.809 kb on + strand, within CCNA_02266 at 2419.926 kb on + strand, within CCNA_02266 at 2419.937 kb on + strand, within CCNA_02266 at 2419.942 kb on + strand, within CCNA_02266 at 2419.962 kb on + strand, within CCNA_02266 at 2419.975 kb on - strand, within CCNA_02266 at 2420.033 kb on - strand, within CCNA_02266 at 2420.054 kb on - strand, within CCNA_02266 at 2420.060 kb on - strand, within CCNA_02266 at 2420.060 kb on - strand, within CCNA_02266 at 2420.060 kb on - strand, within CCNA_02266 at 2420.072 kb on + strand, within CCNA_02266 at 2420.106 kb on + strand, within CCNA_02266 at 2420.106 kb on + strand, within CCNA_02266 at 2420.114 kb on - strand, within CCNA_02266 at 2420.126 kb on + strand, within CCNA_02266
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 2,417,661 + CCNA_02263 0.62 -1.6 2,417,679 + CCNA_02263 0.66 -0.5 2,417,752 - CCNA_02263 0.81 +0.2 2,417,799 + -0.1 2,417,802 - +0.1 2,417,807 - -1.0 2,417,809 + +0.1 2,417,809 + -0.3 2,417,826 + +0.4 2,417,868 + -1.2 2,417,876 - +0.5 2,417,923 + -0.2 2,418,005 + +0.7 2,418,045 - +0.6 2,418,126 - CCNA_02264 0.18 -0.1 2,418,182 + CCNA_02264 0.27 +2.1 2,418,182 + CCNA_02264 0.27 -0.2 2,418,205 + CCNA_02264 0.31 -1.3 2,418,213 - CCNA_02264 0.33 +0.3 2,418,213 - CCNA_02264 0.33 -0.8 2,418,245 - CCNA_02264 0.38 -0.6 2,418,325 + CCNA_02264 0.52 -0.6 2,418,342 + CCNA_02264 0.55 -0.2 2,418,368 + CCNA_02264 0.60 +2.1 2,418,381 - CCNA_02264 0.62 -1.9 2,418,415 + CCNA_02264 0.68 -1.1 2,418,415 + CCNA_02264 0.68 -1.9 2,418,415 + CCNA_02264 0.68 -1.5 2,418,423 - CCNA_02264 0.69 +0.1 2,418,423 - CCNA_02264 0.69 -0.9 2,418,428 - CCNA_02264 0.70 -1.9 2,418,520 - CCNA_02264 0.86 +1.9 2,418,525 + CCNA_02264 0.87 -0.6 2,418,535 + CCNA_02264 0.89 -1.7 2,418,543 - -1.4 2,418,543 - +0.9 2,418,543 - -0.1 2,418,598 + -0.5 2,418,649 + -0.1 2,418,670 + +0.7 2,418,684 + +0.4 2,418,684 + -0.2 2,418,689 + -2.3 2,418,692 - +0.2 2,418,759 - CCNA_02265 0.22 -0.0 2,418,856 + CCNA_02265 0.42 +0.0 2,418,880 - CCNA_02265 0.47 +1.9 2,418,898 - CCNA_02265 0.51 -1.6 2,419,003 + CCNA_02265 0.73 +1.7 2,419,004 + CCNA_02265 0.73 +1.1 2,419,058 - CCNA_02265 0.84 +0.1 2,419,086 + -1.4 2,419,101 + -0.5 2,419,116 - +0.9 2,419,215 + +0.9 2,419,242 - +0.3 2,419,248 - +0.2 2,419,264 + +0.5 2,419,296 + CCNA_02266 0.10 -1.1 2,419,324 - CCNA_02266 0.13 +0.8 2,419,376 - CCNA_02266 0.18 -0.3 2,419,385 - CCNA_02266 0.18 +0.7 2,419,385 - CCNA_02266 0.18 +0.3 2,419,435 - CCNA_02266 0.23 +0.2 2,419,435 - CCNA_02266 0.23 -0.4 2,419,524 + CCNA_02266 0.31 +0.3 2,419,537 - CCNA_02266 0.33 +0.3 2,419,537 - CCNA_02266 0.33 +0.3 2,419,553 - CCNA_02266 0.34 -0.1 2,419,553 - CCNA_02266 0.34 -2.7 2,419,566 - CCNA_02266 0.35 -0.6 2,419,595 + CCNA_02266 0.38 +0.2 2,419,608 + CCNA_02266 0.39 +0.3 2,419,611 + CCNA_02266 0.40 -0.3 2,419,624 - CCNA_02266 0.41 -0.8 2,419,633 - CCNA_02266 0.42 -0.5 2,419,633 - CCNA_02266 0.42 +0.0 2,419,633 - CCNA_02266 0.42 -0.5 2,419,633 - CCNA_02266 0.42 -0.6 2,419,635 + CCNA_02266 0.42 +0.4 2,419,635 + CCNA_02266 0.42 +0.9 2,419,638 - CCNA_02266 0.42 -0.4 2,419,692 + CCNA_02266 0.47 -2.3 2,419,692 + CCNA_02266 0.47 -0.2 2,419,692 + CCNA_02266 0.47 -0.8 2,419,700 - CCNA_02266 0.48 +0.2 2,419,700 - CCNA_02266 0.48 +0.1 2,419,700 - CCNA_02266 0.48 -1.1 2,419,714 - CCNA_02266 0.49 -0.7 2,419,714 - CCNA_02266 0.49 -0.4 2,419,762 + CCNA_02266 0.54 +1.6 2,419,762 + CCNA_02266 0.54 -0.6 2,419,770 - CCNA_02266 0.55 -0.0 2,419,770 - CCNA_02266 0.55 -1.3 2,419,772 + CCNA_02266 0.55 -0.1 2,419,790 + CCNA_02266 0.56 -0.9 2,419,790 + CCNA_02266 0.56 +0.6 2,419,790 + CCNA_02266 0.56 -1.1 2,419,798 - CCNA_02266 0.57 -0.3 2,419,809 + CCNA_02266 0.58 -0.9 2,419,926 + CCNA_02266 0.69 +0.2 2,419,937 + CCNA_02266 0.70 -0.1 2,419,942 + CCNA_02266 0.71 -1.3 2,419,962 + CCNA_02266 0.73 +1.3 2,419,975 - CCNA_02266 0.74 -0.7 2,420,033 - CCNA_02266 0.79 -0.3 2,420,054 - CCNA_02266 0.81 +0.9 2,420,060 - CCNA_02266 0.82 -0.0 2,420,060 - CCNA_02266 0.82 -0.8 2,420,060 - CCNA_02266 0.82 -1.2 2,420,072 + CCNA_02266 0.83 -0.3 2,420,106 + CCNA_02266 0.86 -0.2 2,420,106 + CCNA_02266 0.86 -0.4 2,420,114 - CCNA_02266 0.87 +0.6 2,420,126 + CCNA_02266 0.88 +0.6
Or see this region's nucleotide sequence