Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02250 and CCNA_02251 are separated by 60 nucleotides CCNA_02251 and CCNA_02252 are separated by 21 nucleotides
CCNA_02250: CCNA_02250 - methylcrotonyl-CoA carboxylase biotin-containing subunit, at 2,397,843 to 2,399,807
_02250
CCNA_02251: CCNA_02251 - methylglutaconyl-CoA hydratase, at 2,399,868 to 2,400,707
_02251
CCNA_02252: CCNA_02252 - methylcrotonyl-CoA carboxylase carboxyl transferase subunit, at 2,400,729 to 2,402,321
_02252
Position (kb)
2399
2400
2401 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2398.879 kb on - strand, within CCNA_02250 at 2398.966 kb on - strand, within CCNA_02250 at 2398.993 kb on - strand, within CCNA_02250 at 2399.014 kb on - strand, within CCNA_02250 at 2399.023 kb on + strand, within CCNA_02250 at 2399.023 kb on + strand, within CCNA_02250 at 2399.031 kb on - strand, within CCNA_02250 at 2399.038 kb on + strand, within CCNA_02250 at 2399.273 kb on + strand, within CCNA_02250 at 2399.296 kb on + strand, within CCNA_02250 at 2399.298 kb on - strand, within CCNA_02250 at 2399.309 kb on - strand, within CCNA_02250 at 2399.332 kb on + strand, within CCNA_02250 at 2399.332 kb on + strand, within CCNA_02250 at 2399.332 kb on + strand, within CCNA_02250 at 2399.332 kb on + strand, within CCNA_02250 at 2399.332 kb on + strand, within CCNA_02250 at 2399.332 kb on + strand, within CCNA_02250 at 2399.426 kb on + strand, within CCNA_02250 at 2399.621 kb on + strand at 2399.621 kb on + strand at 2399.621 kb on + strand at 2399.621 kb on + strand at 2399.621 kb on + strand at 2399.668 kb on - strand at 2399.797 kb on + strand at 2399.797 kb on + strand at 2399.800 kb on - strand at 2399.805 kb on - strand at 2399.822 kb on - strand at 2399.845 kb on - strand at 2399.868 kb on - strand at 2400.125 kb on - strand, within CCNA_02251 at 2400.130 kb on - strand, within CCNA_02251 at 2400.244 kb on - strand, within CCNA_02251 at 2400.311 kb on - strand, within CCNA_02251 at 2400.311 kb on - strand, within CCNA_02251 at 2400.329 kb on - strand, within CCNA_02251 at 2400.329 kb on - strand, within CCNA_02251 at 2400.466 kb on - strand, within CCNA_02251 at 2400.533 kb on - strand, within CCNA_02251 at 2400.838 kb on + strand at 2400.838 kb on + strand at 2400.846 kb on - strand at 2400.846 kb on - strand at 2400.846 kb on - strand at 2400.910 kb on + strand, within CCNA_02252 at 2400.923 kb on - strand, within CCNA_02252 at 2401.014 kb on + strand, within CCNA_02252 at 2401.123 kb on + strand, within CCNA_02252 at 2401.123 kb on + strand, within CCNA_02252 at 2401.124 kb on - strand, within CCNA_02252 at 2401.131 kb on - strand, within CCNA_02252 at 2401.133 kb on + strand, within CCNA_02252 at 2401.162 kb on + strand, within CCNA_02252 at 2401.315 kb on + strand, within CCNA_02252 at 2401.323 kb on - strand, within CCNA_02252 at 2401.323 kb on - strand, within CCNA_02252 at 2401.323 kb on - strand, within CCNA_02252 at 2401.348 kb on + strand, within CCNA_02252 at 2401.360 kb on + strand, within CCNA_02252 at 2401.368 kb on - strand, within CCNA_02252 at 2401.383 kb on + strand, within CCNA_02252 at 2401.450 kb on + strand, within CCNA_02252 at 2401.484 kb on - strand, within CCNA_02252 at 2401.592 kb on + strand, within CCNA_02252 at 2401.604 kb on + strand, within CCNA_02252 at 2401.613 kb on - strand, within CCNA_02252 at 2401.621 kb on - strand, within CCNA_02252 at 2401.660 kb on + strand, within CCNA_02252 at 2401.660 kb on + strand, within CCNA_02252 at 2401.668 kb on - strand, within CCNA_02252 at 2401.701 kb on - strand, within CCNA_02252
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 2,398,879 - CCNA_02250 0.53 -2.1 2,398,966 - CCNA_02250 0.57 -1.1 2,398,993 - CCNA_02250 0.59 -0.4 2,399,014 - CCNA_02250 0.60 -0.3 2,399,023 + CCNA_02250 0.60 -0.1 2,399,023 + CCNA_02250 0.60 -0.4 2,399,031 - CCNA_02250 0.60 -1.6 2,399,038 + CCNA_02250 0.61 -1.3 2,399,273 + CCNA_02250 0.73 +0.9 2,399,296 + CCNA_02250 0.74 -2.0 2,399,298 - CCNA_02250 0.74 -0.2 2,399,309 - CCNA_02250 0.75 +0.9 2,399,332 + CCNA_02250 0.76 +0.6 2,399,332 + CCNA_02250 0.76 -1.0 2,399,332 + CCNA_02250 0.76 -2.0 2,399,332 + CCNA_02250 0.76 +1.1 2,399,332 + CCNA_02250 0.76 -0.3 2,399,332 + CCNA_02250 0.76 +0.4 2,399,426 + CCNA_02250 0.81 +0.9 2,399,621 + +0.1 2,399,621 + -2.5 2,399,621 + -0.7 2,399,621 + -0.1 2,399,621 + -1.0 2,399,668 - -1.0 2,399,797 + -0.0 2,399,797 + -1.4 2,399,800 - +0.6 2,399,805 - -0.6 2,399,822 - -0.1 2,399,845 - +0.9 2,399,868 - -0.2 2,400,125 - CCNA_02251 0.31 -1.6 2,400,130 - CCNA_02251 0.31 -2.1 2,400,244 - CCNA_02251 0.45 +0.9 2,400,311 - CCNA_02251 0.53 -1.1 2,400,311 - CCNA_02251 0.53 +0.1 2,400,329 - CCNA_02251 0.55 -1.8 2,400,329 - CCNA_02251 0.55 -0.8 2,400,466 - CCNA_02251 0.71 +1.0 2,400,533 - CCNA_02251 0.79 +1.9 2,400,838 + +1.7 2,400,838 + +1.7 2,400,846 - -0.3 2,400,846 - -0.8 2,400,846 - +0.2 2,400,910 + CCNA_02252 0.11 +1.5 2,400,923 - CCNA_02252 0.12 -1.2 2,401,014 + CCNA_02252 0.18 -0.1 2,401,123 + CCNA_02252 0.25 -2.4 2,401,123 + CCNA_02252 0.25 -0.9 2,401,124 - CCNA_02252 0.25 +0.3 2,401,131 - CCNA_02252 0.25 +0.0 2,401,133 + CCNA_02252 0.25 -0.6 2,401,162 + CCNA_02252 0.27 -1.4 2,401,315 + CCNA_02252 0.37 -1.6 2,401,323 - CCNA_02252 0.37 -0.9 2,401,323 - CCNA_02252 0.37 -0.8 2,401,323 - CCNA_02252 0.37 -1.0 2,401,348 + CCNA_02252 0.39 +0.4 2,401,360 + CCNA_02252 0.40 -2.2 2,401,368 - CCNA_02252 0.40 -1.3 2,401,383 + CCNA_02252 0.41 -1.0 2,401,450 + CCNA_02252 0.45 -0.5 2,401,484 - CCNA_02252 0.47 -0.2 2,401,592 + CCNA_02252 0.54 -1.0 2,401,604 + CCNA_02252 0.55 +1.2 2,401,613 - CCNA_02252 0.55 -0.1 2,401,621 - CCNA_02252 0.56 -2.0 2,401,660 + CCNA_02252 0.58 -1.2 2,401,660 + CCNA_02252 0.58 -0.4 2,401,668 - CCNA_02252 0.59 -1.1 2,401,701 - CCNA_02252 0.61 -1.0
Or see this region's nucleotide sequence