Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02247 and CCNA_02248 are separated by 1 nucleotides CCNA_02248 and CCNA_02249 are separated by 74 nucleotides CCNA_02249 and CCNA_02250 are separated by 207 nucleotides
CCNA_02247: CCNA_02247 - pantoate-beta-alanine ligase, at 2,396,214 to 2,397,071
_02247
CCNA_02248: CCNA_02248 - hypothetical protein, at 2,397,073 to 2,397,162
_02248
CCNA_02249: CCNA_02249 - conserved hypothetical cytosolic protein, at 2,397,237 to 2,397,635
_02249
CCNA_02250: CCNA_02250 - methylcrotonyl-CoA carboxylase biotin-containing subunit, at 2,397,843 to 2,399,807
_02250
Position (kb)
2397
2398 Strain fitness (log2 ratio)
-1
0
1
2 at 2397.108 kb on + strand, within CCNA_02248 at 2397.113 kb on + strand, within CCNA_02248 at 2397.171 kb on + strand at 2397.171 kb on + strand at 2397.179 kb on - strand at 2397.199 kb on - strand at 2397.364 kb on - strand, within CCNA_02249 at 2397.470 kb on - strand, within CCNA_02249 at 2397.470 kb on - strand, within CCNA_02249 at 2397.475 kb on - strand, within CCNA_02249 at 2397.484 kb on + strand, within CCNA_02249 at 2397.484 kb on + strand, within CCNA_02249 at 2397.557 kb on - strand, within CCNA_02249 at 2397.577 kb on + strand, within CCNA_02249 at 2397.614 kb on + strand at 2397.624 kb on + strand at 2397.627 kb on - strand at 2397.637 kb on - strand at 2397.639 kb on - strand at 2397.646 kb on - strand at 2397.659 kb on + strand at 2397.667 kb on - strand at 2397.771 kb on - strand at 2397.771 kb on - strand at 2397.771 kb on - strand at 2397.846 kb on + strand at 2397.846 kb on + strand at 2397.849 kb on - strand at 2397.865 kb on - strand at 2397.867 kb on + strand at 2397.867 kb on + strand at 2397.875 kb on - strand at 2397.943 kb on + strand at 2397.968 kb on + strand at 2397.984 kb on + strand at 2398.025 kb on - strand at 2398.124 kb on - strand, within CCNA_02250 at 2398.238 kb on - strand, within CCNA_02250 at 2398.238 kb on - strand, within CCNA_02250 at 2398.248 kb on + strand, within CCNA_02250 at 2398.263 kb on - strand, within CCNA_02250 at 2398.486 kb on - strand, within CCNA_02250 at 2398.632 kb on - strand, within CCNA_02250
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 2,397,108 + CCNA_02248 0.39 -1.6 2,397,113 + CCNA_02248 0.44 -1.4 2,397,171 + +0.4 2,397,171 + -0.4 2,397,179 - +1.9 2,397,199 - +0.0 2,397,364 - CCNA_02249 0.32 +0.4 2,397,470 - CCNA_02249 0.58 +0.3 2,397,470 - CCNA_02249 0.58 +0.2 2,397,475 - CCNA_02249 0.60 +0.4 2,397,484 + CCNA_02249 0.62 +0.2 2,397,484 + CCNA_02249 0.62 +1.2 2,397,557 - CCNA_02249 0.80 +0.5 2,397,577 + CCNA_02249 0.85 +0.1 2,397,614 + -0.7 2,397,624 + -1.5 2,397,627 - +0.3 2,397,637 - +0.8 2,397,639 - +0.4 2,397,646 - +1.4 2,397,659 + -0.4 2,397,667 - +0.5 2,397,771 - +1.7 2,397,771 - +0.3 2,397,771 - -0.3 2,397,846 + +0.9 2,397,846 + -0.6 2,397,849 - +0.1 2,397,865 - +0.1 2,397,867 + +1.0 2,397,867 + -1.4 2,397,875 - +0.6 2,397,943 + -1.4 2,397,968 + -1.6 2,397,984 + -0.3 2,398,025 - -0.4 2,398,124 - CCNA_02250 0.14 -0.3 2,398,238 - CCNA_02250 0.20 -0.4 2,398,238 - CCNA_02250 0.20 +1.1 2,398,248 + CCNA_02250 0.21 +1.9 2,398,263 - CCNA_02250 0.21 -0.3 2,398,486 - CCNA_02250 0.33 +1.9 2,398,632 - CCNA_02250 0.40 -0.3
Or see this region's nucleotide sequence