Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02035 and CCNA_R0047 are separated by 302 nucleotides CCNA_R0047 and CCNA_02036 are separated by 106 nucleotides CCNA_02036 and CCNA_02037 are separated by 143 nucleotides
CCNA_02035: CCNA_02035 - glycosyltransferase, at 2,178,958 to 2,180,166
_02035
CCNA_R0047: CCNA_R0047 - tRNA-Val, at 2,180,469 to 2,180,543
_R0047
CCNA_02036: CCNA_02036 - DNA-binding protein HU, at 2,180,650 to 2,180,928
_02036
CCNA_02037: CCNA_02037 - ATP-dependent endopeptidase Lon, at 2,181,072 to 2,183,471
_02037
Position (kb)
2180
2181 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2179.678 kb on - strand, within CCNA_02035 at 2179.748 kb on - strand, within CCNA_02035 at 2179.860 kb on + strand, within CCNA_02035 at 2179.888 kb on + strand, within CCNA_02035 at 2179.896 kb on - strand, within CCNA_02035 at 2179.927 kb on - strand, within CCNA_02035 at 2179.927 kb on - strand, within CCNA_02035 at 2179.980 kb on - strand, within CCNA_02035 at 2179.999 kb on + strand, within CCNA_02035 at 2179.999 kb on + strand, within CCNA_02035 at 2179.999 kb on + strand, within CCNA_02035 at 2180.007 kb on - strand, within CCNA_02035 at 2180.075 kb on - strand at 2180.130 kb on - strand at 2180.133 kb on + strand at 2180.133 kb on + strand at 2180.141 kb on - strand at 2180.141 kb on - strand at 2180.150 kb on + strand at 2180.158 kb on - strand at 2180.240 kb on + strand at 2180.258 kb on - strand at 2180.258 kb on - strand at 2180.271 kb on + strand at 2180.271 kb on + strand at 2180.287 kb on - strand at 2180.304 kb on - strand at 2180.345 kb on + strand at 2180.347 kb on - strand at 2180.384 kb on + strand at 2180.392 kb on - strand at 2180.394 kb on + strand at 2180.394 kb on + strand at 2180.397 kb on - strand at 2180.402 kb on - strand at 2180.402 kb on - strand at 2180.402 kb on - strand at 2180.416 kb on + strand at 2180.460 kb on - strand at 2180.471 kb on - strand at 2180.521 kb on + strand, within CCNA_R0047 at 2180.521 kb on + strand, within CCNA_R0047 at 2180.566 kb on + strand at 2180.615 kb on - strand at 2180.668 kb on + strand at 2180.668 kb on + strand at 2180.668 kb on - strand at 2180.676 kb on - strand at 2180.772 kb on - strand, within CCNA_02036 at 2180.779 kb on - strand, within CCNA_02036 at 2180.824 kb on + strand, within CCNA_02036 at 2180.832 kb on - strand, within CCNA_02036 at 2180.832 kb on - strand, within CCNA_02036 at 2180.866 kb on - strand, within CCNA_02036 at 2180.866 kb on - strand, within CCNA_02036 at 2180.866 kb on - strand, within CCNA_02036 at 2180.935 kb on + strand at 2180.943 kb on - strand at 2181.019 kb on - strand at 2181.026 kb on - strand at 2181.147 kb on - strand at 2181.157 kb on + strand at 2181.171 kb on + strand at 2181.176 kb on + strand at 2181.189 kb on - strand at 2181.383 kb on - strand, within CCNA_02037
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 2,179,678 - CCNA_02035 0.60 -0.1 2,179,748 - CCNA_02035 0.65 -0.5 2,179,860 + CCNA_02035 0.75 +0.2 2,179,888 + CCNA_02035 0.77 -1.7 2,179,896 - CCNA_02035 0.78 +1.0 2,179,927 - CCNA_02035 0.80 +0.4 2,179,927 - CCNA_02035 0.80 +1.2 2,179,980 - CCNA_02035 0.85 -0.1 2,179,999 + CCNA_02035 0.86 -0.4 2,179,999 + CCNA_02035 0.86 +1.8 2,179,999 + CCNA_02035 0.86 -1.0 2,180,007 - CCNA_02035 0.87 +0.3 2,180,075 - +0.7 2,180,130 - -0.9 2,180,133 + +0.9 2,180,133 + -3.1 2,180,141 - -0.5 2,180,141 - -0.1 2,180,150 + -1.5 2,180,158 - +0.4 2,180,240 + -0.1 2,180,258 - +0.9 2,180,258 - +0.2 2,180,271 + +1.3 2,180,271 + +0.9 2,180,287 - -0.3 2,180,304 - +0.4 2,180,345 + -0.8 2,180,347 - +0.1 2,180,384 + -0.5 2,180,392 - -2.2 2,180,394 + -1.4 2,180,394 + -1.4 2,180,397 - +0.6 2,180,402 - +0.1 2,180,402 - -0.7 2,180,402 - -0.3 2,180,416 + -0.6 2,180,460 - -0.4 2,180,471 - -0.2 2,180,521 + CCNA_R0047 0.69 -0.6 2,180,521 + CCNA_R0047 0.69 -1.0 2,180,566 + -0.5 2,180,615 - -0.8 2,180,668 + -0.3 2,180,668 + -0.4 2,180,668 - +0.3 2,180,676 - +1.1 2,180,772 - CCNA_02036 0.44 +0.7 2,180,779 - CCNA_02036 0.46 +0.7 2,180,824 + CCNA_02036 0.62 +0.7 2,180,832 - CCNA_02036 0.65 +0.6 2,180,832 - CCNA_02036 0.65 +0.7 2,180,866 - CCNA_02036 0.77 +0.7 2,180,866 - CCNA_02036 0.77 +0.9 2,180,866 - CCNA_02036 0.77 +0.7 2,180,935 + +0.2 2,180,943 - +0.4 2,181,019 - +0.1 2,181,026 - -0.2 2,181,147 - +0.0 2,181,157 + +0.5 2,181,171 + -2.6 2,181,176 + +0.6 2,181,189 - +0.3 2,181,383 - CCNA_02037 0.13 +0.1
Or see this region's nucleotide sequence