Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01975 and CCNA_01976 overlap by 4 nucleotides CCNA_01976 and CCNA_01977 are separated by 95 nucleotides CCNA_01977 and CCNA_01978 are separated by 50 nucleotides
CCNA_01975: CCNA_01975 - anthranilate phosphoribosyltransferase, at 2,119,875 to 2,120,900
_01975
CCNA_01976: CCNA_01976 - Indole-3-glycerol phosphate synthase, at 2,120,897 to 2,121,685
_01976
CCNA_01977: CCNA_01977 - 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 2,121,781 to 2,122,371
_01977
CCNA_01978: CCNA_01978 - methyltransferase, at 2,122,422 to 2,123,273
_01978
Position (kb)
2120
2121
2122 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2119.917 kb on + strand at 2119.925 kb on - strand at 2120.093 kb on + strand, within CCNA_01975 at 2120.095 kb on + strand, within CCNA_01975 at 2120.141 kb on - strand, within CCNA_01975 at 2120.156 kb on - strand, within CCNA_01975 at 2120.187 kb on + strand, within CCNA_01975 at 2120.247 kb on + strand, within CCNA_01975 at 2120.309 kb on - strand, within CCNA_01975 at 2120.400 kb on + strand, within CCNA_01975 at 2120.400 kb on + strand, within CCNA_01975 at 2120.409 kb on - strand, within CCNA_01975 at 2120.410 kb on - strand, within CCNA_01975 at 2120.496 kb on + strand, within CCNA_01975 at 2120.503 kb on - strand, within CCNA_01975 at 2120.539 kb on + strand, within CCNA_01975 at 2120.540 kb on - strand, within CCNA_01975 at 2120.544 kb on + strand, within CCNA_01975 at 2120.637 kb on + strand, within CCNA_01975 at 2120.642 kb on + strand, within CCNA_01975 at 2120.661 kb on + strand, within CCNA_01975 at 2120.705 kb on - strand, within CCNA_01975 at 2120.758 kb on - strand, within CCNA_01975 at 2120.787 kb on + strand, within CCNA_01975 at 2120.794 kb on - strand, within CCNA_01975 at 2120.834 kb on - strand at 2120.989 kb on + strand, within CCNA_01976 at 2121.027 kb on + strand, within CCNA_01976 at 2121.049 kb on + strand, within CCNA_01976 at 2121.062 kb on - strand, within CCNA_01976 at 2121.165 kb on + strand, within CCNA_01976 at 2121.171 kb on - strand, within CCNA_01976 at 2121.295 kb on - strand, within CCNA_01976 at 2121.320 kb on - strand, within CCNA_01976 at 2121.367 kb on - strand, within CCNA_01976 at 2121.407 kb on + strand, within CCNA_01976 at 2121.426 kb on - strand, within CCNA_01976 at 2121.452 kb on + strand, within CCNA_01976 at 2121.479 kb on + strand, within CCNA_01976 at 2121.585 kb on - strand, within CCNA_01976 at 2121.641 kb on - strand at 2121.745 kb on - strand at 2121.786 kb on - strand at 2121.791 kb on - strand at 2121.804 kb on + strand at 2121.837 kb on + strand at 2121.895 kb on + strand, within CCNA_01977 at 2121.903 kb on - strand, within CCNA_01977 at 2121.915 kb on - strand, within CCNA_01977 at 2121.950 kb on + strand, within CCNA_01977 at 2122.054 kb on - strand, within CCNA_01977 at 2122.065 kb on + strand, within CCNA_01977 at 2122.239 kb on - strand, within CCNA_01977 at 2122.260 kb on + strand, within CCNA_01977 at 2122.260 kb on + strand, within CCNA_01977 at 2122.353 kb on - strand at 2122.360 kb on - strand at 2122.376 kb on + strand at 2122.379 kb on - strand at 2122.386 kb on + strand at 2122.394 kb on - strand at 2122.410 kb on - strand at 2122.449 kb on - strand at 2122.518 kb on - strand, within CCNA_01978 at 2122.522 kb on + strand, within CCNA_01978 at 2122.522 kb on + strand, within CCNA_01978 at 2122.543 kb on - strand, within CCNA_01978 at 2122.630 kb on - strand, within CCNA_01978 at 2122.630 kb on - strand, within CCNA_01978 at 2122.630 kb on - strand, within CCNA_01978
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 2,119,917 + +0.0 2,119,925 - -0.5 2,120,093 + CCNA_01975 0.21 +0.3 2,120,095 + CCNA_01975 0.21 +0.4 2,120,141 - CCNA_01975 0.26 -0.3 2,120,156 - CCNA_01975 0.27 +0.7 2,120,187 + CCNA_01975 0.30 +2.2 2,120,247 + CCNA_01975 0.36 +1.1 2,120,309 - CCNA_01975 0.42 -2.2 2,120,400 + CCNA_01975 0.51 +0.3 2,120,400 + CCNA_01975 0.51 +0.3 2,120,409 - CCNA_01975 0.52 +1.0 2,120,410 - CCNA_01975 0.52 +0.4 2,120,496 + CCNA_01975 0.61 +0.5 2,120,503 - CCNA_01975 0.61 -0.3 2,120,539 + CCNA_01975 0.65 +0.2 2,120,540 - CCNA_01975 0.65 -1.4 2,120,544 + CCNA_01975 0.65 +0.3 2,120,637 + CCNA_01975 0.74 +0.8 2,120,642 + CCNA_01975 0.75 +0.7 2,120,661 + CCNA_01975 0.77 +0.9 2,120,705 - CCNA_01975 0.81 -0.2 2,120,758 - CCNA_01975 0.86 -0.0 2,120,787 + CCNA_01975 0.89 +0.4 2,120,794 - CCNA_01975 0.90 -1.9 2,120,834 - -0.8 2,120,989 + CCNA_01976 0.12 -0.8 2,121,027 + CCNA_01976 0.16 -0.4 2,121,049 + CCNA_01976 0.19 -0.1 2,121,062 - CCNA_01976 0.21 +0.1 2,121,165 + CCNA_01976 0.34 +1.2 2,121,171 - CCNA_01976 0.35 +2.1 2,121,295 - CCNA_01976 0.50 +2.2 2,121,320 - CCNA_01976 0.54 -0.8 2,121,367 - CCNA_01976 0.60 -1.5 2,121,407 + CCNA_01976 0.65 +0.3 2,121,426 - CCNA_01976 0.67 +0.6 2,121,452 + CCNA_01976 0.70 +1.0 2,121,479 + CCNA_01976 0.74 +0.5 2,121,585 - CCNA_01976 0.87 +0.1 2,121,641 - -0.4 2,121,745 - -0.0 2,121,786 - -0.6 2,121,791 - -0.7 2,121,804 + +0.3 2,121,837 + -0.5 2,121,895 + CCNA_01977 0.19 -0.4 2,121,903 - CCNA_01977 0.21 -0.6 2,121,915 - CCNA_01977 0.23 -0.0 2,121,950 + CCNA_01977 0.29 -1.9 2,122,054 - CCNA_01977 0.46 +0.6 2,122,065 + CCNA_01977 0.48 -0.5 2,122,239 - CCNA_01977 0.77 +0.2 2,122,260 + CCNA_01977 0.81 +0.2 2,122,260 + CCNA_01977 0.81 +0.7 2,122,353 - +0.5 2,122,360 - -0.3 2,122,376 + -0.1 2,122,379 - +0.1 2,122,386 + +0.8 2,122,394 - -0.7 2,122,410 - -0.9 2,122,449 - +0.4 2,122,518 - CCNA_01978 0.11 +0.8 2,122,522 + CCNA_01978 0.12 +1.7 2,122,522 + CCNA_01978 0.12 +0.4 2,122,543 - CCNA_01978 0.14 -0.6 2,122,630 - CCNA_01978 0.24 -0.8 2,122,630 - CCNA_01978 0.24 -0.3 2,122,630 - CCNA_01978 0.24 -0.1
Or see this region's nucleotide sequence