Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01961 and CCNA_01962 are separated by 99 nucleotides CCNA_01962 and CCNA_01963 overlap by 64 nucleotides CCNA_01963 and CCNA_01964 are separated by 4 nucleotides
CCNA_01961: CCNA_01961 - biotin carboxylase, at 2,104,868 to 2,106,214
_01961
CCNA_01962: CCNA_01962 - leucyl/phenylalanyl-tRNA--protein transferase, at 2,106,314 to 2,106,967
_01962
CCNA_01963: CCNA_01963 - hypothetical protein, at 2,106,904 to 2,107,542
_01963
CCNA_01964: CCNA_01964 - NADH dehydrogenase, at 2,107,547 to 2,107,942
_01964
Position (kb)
2106
2107 Strain fitness (log2 ratio)
-1
0
1
2 at 2106.288 kb on + strand at 2106.288 kb on + strand at 2106.288 kb on + strand at 2106.288 kb on + strand at 2106.296 kb on - strand at 2106.306 kb on - strand at 2106.308 kb on - strand at 2106.414 kb on + strand, within CCNA_01962 at 2106.422 kb on - strand, within CCNA_01962 at 2106.464 kb on + strand, within CCNA_01962 at 2106.479 kb on + strand, within CCNA_01962 at 2106.513 kb on - strand, within CCNA_01962 at 2106.515 kb on + strand, within CCNA_01962 at 2106.523 kb on - strand, within CCNA_01962 at 2106.570 kb on - strand, within CCNA_01962 at 2106.615 kb on - strand, within CCNA_01962 at 2106.615 kb on - strand, within CCNA_01962 at 2106.616 kb on - strand, within CCNA_01962 at 2106.635 kb on + strand, within CCNA_01962 at 2106.641 kb on - strand, within CCNA_01962 at 2106.643 kb on - strand, within CCNA_01962 at 2106.651 kb on + strand, within CCNA_01962 at 2106.715 kb on + strand, within CCNA_01962 at 2106.791 kb on - strand, within CCNA_01962 at 2106.813 kb on - strand, within CCNA_01962 at 2106.829 kb on + strand, within CCNA_01962 at 2106.874 kb on + strand, within CCNA_01962 at 2106.875 kb on + strand, within CCNA_01962 at 2106.882 kb on - strand, within CCNA_01962 at 2106.985 kb on + strand, within CCNA_01963 at 2107.011 kb on - strand, within CCNA_01963 at 2107.017 kb on - strand, within CCNA_01963 at 2107.017 kb on - strand, within CCNA_01963 at 2107.017 kb on - strand, within CCNA_01963 at 2107.343 kb on - strand, within CCNA_01963 at 2107.351 kb on + strand, within CCNA_01963 at 2107.387 kb on - strand, within CCNA_01963 at 2107.519 kb on + strand at 2107.577 kb on + strand at 2107.623 kb on + strand, within CCNA_01964
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 2,106,288 + +0.3 2,106,288 + +0.4 2,106,288 + +0.3 2,106,288 + +1.0 2,106,296 - +0.7 2,106,306 - -1.3 2,106,308 - +0.3 2,106,414 + CCNA_01962 0.15 +0.7 2,106,422 - CCNA_01962 0.17 +0.4 2,106,464 + CCNA_01962 0.23 +0.4 2,106,479 + CCNA_01962 0.25 +0.6 2,106,513 - CCNA_01962 0.30 -0.9 2,106,515 + CCNA_01962 0.31 +1.7 2,106,523 - CCNA_01962 0.32 -1.4 2,106,570 - CCNA_01962 0.39 -0.6 2,106,615 - CCNA_01962 0.46 +0.4 2,106,615 - CCNA_01962 0.46 +0.3 2,106,616 - CCNA_01962 0.46 +0.2 2,106,635 + CCNA_01962 0.49 +2.2 2,106,641 - CCNA_01962 0.50 -1.0 2,106,643 - CCNA_01962 0.50 +0.0 2,106,651 + CCNA_01962 0.52 -0.1 2,106,715 + CCNA_01962 0.61 +0.8 2,106,791 - CCNA_01962 0.73 +1.6 2,106,813 - CCNA_01962 0.76 +0.8 2,106,829 + CCNA_01962 0.79 -0.7 2,106,874 + CCNA_01962 0.86 +2.0 2,106,875 + CCNA_01962 0.86 +0.6 2,106,882 - CCNA_01962 0.87 -0.3 2,106,985 + CCNA_01963 0.13 -1.0 2,107,011 - CCNA_01963 0.17 +0.1 2,107,017 - CCNA_01963 0.18 +0.1 2,107,017 - CCNA_01963 0.18 +0.7 2,107,017 - CCNA_01963 0.18 +0.5 2,107,343 - CCNA_01963 0.69 -0.2 2,107,351 + CCNA_01963 0.70 +0.1 2,107,387 - CCNA_01963 0.76 +0.4 2,107,519 + +0.6 2,107,577 + +0.2 2,107,623 + CCNA_01964 0.19 -0.8
Or see this region's nucleotide sequence