Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01927 and CCNA_01928 overlap by 14 nucleotides CCNA_01928 and CCNA_01929 overlap by 4 nucleotides CCNA_01929 and CCNA_01930 are separated by 0 nucleotides
CCNA_01927: CCNA_01927 - enoyl-CoA hydratase, at 2,073,818 to 2,074,621
_01927
CCNA_01928: CCNA_01928 - conserved ACT domain protein, at 2,074,608 to 2,075,018
_01928
CCNA_01929: CCNA_01929 - hypothetical protein, at 2,075,015 to 2,075,419
_01929
CCNA_01930: CCNA_01930 - ADP-heptose LPS heptosyltransferase, at 2,075,420 to 2,076,391
_01930
Position (kb)
2075
2076 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2074.148 kb on + strand, within CCNA_01927 at 2074.148 kb on + strand, within CCNA_01927 at 2074.148 kb on + strand, within CCNA_01927 at 2074.177 kb on - strand, within CCNA_01927 at 2074.232 kb on - strand, within CCNA_01927 at 2074.256 kb on - strand, within CCNA_01927 at 2074.257 kb on - strand, within CCNA_01927 at 2074.279 kb on - strand, within CCNA_01927 at 2074.436 kb on - strand, within CCNA_01927 at 2074.446 kb on - strand, within CCNA_01927 at 2074.446 kb on - strand, within CCNA_01927 at 2074.485 kb on - strand, within CCNA_01927 at 2074.485 kb on - strand, within CCNA_01927 at 2074.485 kb on - strand, within CCNA_01927 at 2074.486 kb on - strand, within CCNA_01927 at 2074.496 kb on - strand, within CCNA_01927 at 2074.513 kb on + strand, within CCNA_01927 at 2074.532 kb on + strand, within CCNA_01927 at 2074.586 kb on + strand at 2074.594 kb on - strand at 2074.604 kb on + strand at 2074.606 kb on + strand at 2074.614 kb on - strand at 2074.614 kb on - strand at 2074.632 kb on - strand at 2074.637 kb on + strand at 2074.679 kb on - strand, within CCNA_01928 at 2074.688 kb on - strand, within CCNA_01928 at 2074.698 kb on + strand, within CCNA_01928 at 2074.769 kb on - strand, within CCNA_01928 at 2074.784 kb on - strand, within CCNA_01928 at 2074.784 kb on - strand, within CCNA_01928 at 2074.803 kb on + strand, within CCNA_01928 at 2074.803 kb on + strand, within CCNA_01928 at 2074.803 kb on + strand, within CCNA_01928 at 2074.803 kb on + strand, within CCNA_01928 at 2074.811 kb on - strand, within CCNA_01928 at 2074.854 kb on + strand, within CCNA_01928 at 2074.863 kb on + strand, within CCNA_01928 at 2075.026 kb on - strand at 2075.228 kb on + strand, within CCNA_01929 at 2075.228 kb on + strand, within CCNA_01929 at 2075.261 kb on - strand, within CCNA_01929 at 2075.360 kb on - strand, within CCNA_01929 at 2075.394 kb on + strand at 2075.412 kb on - strand at 2075.461 kb on - strand at 2075.462 kb on - strand at 2075.654 kb on - strand, within CCNA_01930 at 2075.679 kb on + strand, within CCNA_01930 at 2075.714 kb on - strand, within CCNA_01930 at 2075.790 kb on + strand, within CCNA_01930 at 2075.829 kb on - strand, within CCNA_01930 at 2075.829 kb on - strand, within CCNA_01930 at 2075.846 kb on + strand, within CCNA_01930 at 2075.849 kb on - strand, within CCNA_01930 at 2075.855 kb on - strand, within CCNA_01930 at 2075.929 kb on + strand, within CCNA_01930 at 2075.938 kb on + strand, within CCNA_01930 at 2075.947 kb on - strand, within CCNA_01930 at 2075.947 kb on - strand, within CCNA_01930 at 2075.952 kb on - strand, within CCNA_01930 at 2075.966 kb on - strand, within CCNA_01930 at 2075.973 kb on + strand, within CCNA_01930 at 2076.127 kb on + strand, within CCNA_01930 at 2076.151 kb on + strand, within CCNA_01930 at 2076.159 kb on - strand, within CCNA_01930 at 2076.202 kb on + strand, within CCNA_01930 at 2076.244 kb on + strand, within CCNA_01930 at 2076.244 kb on + strand, within CCNA_01930 at 2076.258 kb on + strand, within CCNA_01930 at 2076.258 kb on + strand, within CCNA_01930 at 2076.266 kb on - strand, within CCNA_01930 at 2076.313 kb on + strand at 2076.386 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 2,074,148 + CCNA_01927 0.41 +0.3 2,074,148 + CCNA_01927 0.41 +1.4 2,074,148 + CCNA_01927 0.41 +0.7 2,074,177 - CCNA_01927 0.45 -0.1 2,074,232 - CCNA_01927 0.51 +0.6 2,074,256 - CCNA_01927 0.54 -0.7 2,074,257 - CCNA_01927 0.55 -1.0 2,074,279 - CCNA_01927 0.57 +0.2 2,074,436 - CCNA_01927 0.77 -0.6 2,074,446 - CCNA_01927 0.78 -0.3 2,074,446 - CCNA_01927 0.78 -1.5 2,074,485 - CCNA_01927 0.83 -0.5 2,074,485 - CCNA_01927 0.83 +0.0 2,074,485 - CCNA_01927 0.83 -1.8 2,074,486 - CCNA_01927 0.83 +0.2 2,074,496 - CCNA_01927 0.84 +0.3 2,074,513 + CCNA_01927 0.86 +0.7 2,074,532 + CCNA_01927 0.89 +0.6 2,074,586 + -0.2 2,074,594 - +0.3 2,074,604 + -0.1 2,074,606 + +0.5 2,074,614 - -0.4 2,074,614 - +0.4 2,074,632 - -0.5 2,074,637 + +0.1 2,074,679 - CCNA_01928 0.17 -1.4 2,074,688 - CCNA_01928 0.19 -1.5 2,074,698 + CCNA_01928 0.22 -1.5 2,074,769 - CCNA_01928 0.39 +1.2 2,074,784 - CCNA_01928 0.43 -1.0 2,074,784 - CCNA_01928 0.43 +0.3 2,074,803 + CCNA_01928 0.47 +0.5 2,074,803 + CCNA_01928 0.47 -1.8 2,074,803 + CCNA_01928 0.47 +1.5 2,074,803 + CCNA_01928 0.47 +1.7 2,074,811 - CCNA_01928 0.49 +2.4 2,074,854 + CCNA_01928 0.60 -1.1 2,074,863 + CCNA_01928 0.62 -0.2 2,075,026 - +0.0 2,075,228 + CCNA_01929 0.53 -0.5 2,075,228 + CCNA_01929 0.53 +0.3 2,075,261 - CCNA_01929 0.61 +1.3 2,075,360 - CCNA_01929 0.85 +0.2 2,075,394 + +0.3 2,075,412 - +1.0 2,075,461 - -0.2 2,075,462 - -1.8 2,075,654 - CCNA_01930 0.24 -0.3 2,075,679 + CCNA_01930 0.27 +0.4 2,075,714 - CCNA_01930 0.30 -1.1 2,075,790 + CCNA_01930 0.38 +0.8 2,075,829 - CCNA_01930 0.42 +0.3 2,075,829 - CCNA_01930 0.42 -0.4 2,075,846 + CCNA_01930 0.44 -0.7 2,075,849 - CCNA_01930 0.44 +0.6 2,075,855 - CCNA_01930 0.45 -0.2 2,075,929 + CCNA_01930 0.52 -0.8 2,075,938 + CCNA_01930 0.53 -0.3 2,075,947 - CCNA_01930 0.54 -0.3 2,075,947 - CCNA_01930 0.54 -0.8 2,075,952 - CCNA_01930 0.55 +0.1 2,075,966 - CCNA_01930 0.56 -0.1 2,075,973 + CCNA_01930 0.57 -0.5 2,076,127 + CCNA_01930 0.73 +0.4 2,076,151 + CCNA_01930 0.75 -0.8 2,076,159 - CCNA_01930 0.76 -0.4 2,076,202 + CCNA_01930 0.80 +0.2 2,076,244 + CCNA_01930 0.85 +0.8 2,076,244 + CCNA_01930 0.85 -0.4 2,076,258 + CCNA_01930 0.86 -0.0 2,076,258 + CCNA_01930 0.86 -0.8 2,076,266 - CCNA_01930 0.87 -0.0 2,076,313 + -0.2 2,076,386 + -1.7
Or see this region's nucleotide sequence