Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01803 and CCNA_01804 are separated by 18 nucleotides CCNA_01804 and CCNA_01805 are separated by 82 nucleotides CCNA_01805 and CCNA_01806 are separated by 19 nucleotides
CCNA_01803: CCNA_01803 - pyruvate dehydrogenase complex, dihydrolipoamide acetyltransferase component, at 1,926,921 to 1,928,207
_01803
CCNA_01804: CCNA_01804 - glutathione peroxidase, at 1,928,226 to 1,928,708
_01804
CCNA_01805: CCNA_01805 - dihydrolipoamide dehydrogenase, at 1,928,791 to 1,930,191
_01805
CCNA_01806: CCNA_01806 - ferritin-like domain protein, at 1,930,211 to 1,931,668
_01806
Position (kb)
1928
1929
1930
1931 Strain fitness (log2 ratio)
-1
0
1
2 at 1927.872 kb on + strand, within CCNA_01803 at 1927.897 kb on + strand, within CCNA_01803 at 1928.200 kb on - strand at 1928.223 kb on + strand at 1928.372 kb on + strand, within CCNA_01804 at 1928.395 kb on + strand, within CCNA_01804 at 1928.403 kb on + strand, within CCNA_01804 at 1928.403 kb on + strand, within CCNA_01804 at 1928.403 kb on + strand, within CCNA_01804 at 1928.411 kb on - strand, within CCNA_01804 at 1928.411 kb on - strand, within CCNA_01804 at 1928.431 kb on + strand, within CCNA_01804 at 1928.444 kb on + strand, within CCNA_01804 at 1928.444 kb on + strand, within CCNA_01804 at 1928.480 kb on + strand, within CCNA_01804 at 1928.482 kb on + strand, within CCNA_01804 at 1928.520 kb on - strand, within CCNA_01804 at 1928.534 kb on + strand, within CCNA_01804 at 1928.544 kb on + strand, within CCNA_01804 at 1928.565 kb on + strand, within CCNA_01804 at 1928.565 kb on + strand, within CCNA_01804 at 1928.587 kb on - strand, within CCNA_01804 at 1928.702 kb on + strand at 1928.702 kb on + strand at 1928.702 kb on + strand at 1928.702 kb on + strand at 1928.702 kb on + strand at 1928.702 kb on + strand at 1928.702 kb on + strand at 1928.710 kb on - strand at 1928.722 kb on + strand at 1928.730 kb on - strand at 1928.985 kb on + strand, within CCNA_01805 at 1929.115 kb on - strand, within CCNA_01805 at 1929.217 kb on + strand, within CCNA_01805 at 1929.282 kb on + strand, within CCNA_01805 at 1930.261 kb on - strand at 1930.271 kb on - strand at 1930.329 kb on + strand at 1930.356 kb on - strand at 1930.371 kb on + strand, within CCNA_01806 at 1930.371 kb on + strand, within CCNA_01806 at 1930.529 kb on + strand, within CCNA_01806 at 1930.529 kb on + strand, within CCNA_01806 at 1930.592 kb on + strand, within CCNA_01806 at 1930.595 kb on - strand, within CCNA_01806 at 1930.613 kb on + strand, within CCNA_01806 at 1930.644 kb on - strand, within CCNA_01806 at 1930.678 kb on + strand, within CCNA_01806 at 1930.686 kb on - strand, within CCNA_01806 at 1930.723 kb on + strand, within CCNA_01806 at 1930.784 kb on + strand, within CCNA_01806 at 1930.812 kb on - strand, within CCNA_01806 at 1930.857 kb on + strand, within CCNA_01806 at 1930.937 kb on - strand, within CCNA_01806 at 1930.947 kb on + strand, within CCNA_01806 at 1931.036 kb on + strand, within CCNA_01806 at 1931.068 kb on - strand, within CCNA_01806 at 1931.110 kb on + strand, within CCNA_01806 at 1931.133 kb on + strand, within CCNA_01806 at 1931.141 kb on - strand, within CCNA_01806 at 1931.141 kb on - strand, within CCNA_01806 at 1931.164 kb on + strand, within CCNA_01806
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 1,927,872 + CCNA_01803 0.74 -0.3 1,927,897 + CCNA_01803 0.76 +0.7 1,928,200 - -0.7 1,928,223 + +1.3 1,928,372 + CCNA_01804 0.30 -0.6 1,928,395 + CCNA_01804 0.35 -1.0 1,928,403 + CCNA_01804 0.37 +0.8 1,928,403 + CCNA_01804 0.37 +0.1 1,928,403 + CCNA_01804 0.37 +0.9 1,928,411 - CCNA_01804 0.38 -0.7 1,928,411 - CCNA_01804 0.38 -0.2 1,928,431 + CCNA_01804 0.42 +0.1 1,928,444 + CCNA_01804 0.45 +0.9 1,928,444 + CCNA_01804 0.45 +1.3 1,928,480 + CCNA_01804 0.53 +0.6 1,928,482 + CCNA_01804 0.53 -0.3 1,928,520 - CCNA_01804 0.61 +1.9 1,928,534 + CCNA_01804 0.64 -0.6 1,928,544 + CCNA_01804 0.66 +0.7 1,928,565 + CCNA_01804 0.70 +0.5 1,928,565 + CCNA_01804 0.70 +0.9 1,928,587 - CCNA_01804 0.75 -0.9 1,928,702 + +0.7 1,928,702 + +0.4 1,928,702 + +0.4 1,928,702 + +0.1 1,928,702 + +0.0 1,928,702 + +0.9 1,928,702 + +0.0 1,928,710 - -0.8 1,928,722 + +0.1 1,928,730 - -1.4 1,928,985 + CCNA_01805 0.14 -0.1 1,929,115 - CCNA_01805 0.23 -0.1 1,929,217 + CCNA_01805 0.30 -0.1 1,929,282 + CCNA_01805 0.35 -0.6 1,930,261 - -1.5 1,930,271 - -0.7 1,930,329 + +0.7 1,930,356 - +0.7 1,930,371 + CCNA_01806 0.11 -0.4 1,930,371 + CCNA_01806 0.11 +0.5 1,930,529 + CCNA_01806 0.22 -0.3 1,930,529 + CCNA_01806 0.22 +0.4 1,930,592 + CCNA_01806 0.26 +0.7 1,930,595 - CCNA_01806 0.26 -0.0 1,930,613 + CCNA_01806 0.28 +0.3 1,930,644 - CCNA_01806 0.30 -0.7 1,930,678 + CCNA_01806 0.32 +0.2 1,930,686 - CCNA_01806 0.33 -0.8 1,930,723 + CCNA_01806 0.35 +0.2 1,930,784 + CCNA_01806 0.39 +0.4 1,930,812 - CCNA_01806 0.41 -0.6 1,930,857 + CCNA_01806 0.44 -0.1 1,930,937 - CCNA_01806 0.50 -0.4 1,930,947 + CCNA_01806 0.50 +0.2 1,931,036 + CCNA_01806 0.57 -0.6 1,931,068 - CCNA_01806 0.59 -0.4 1,931,110 + CCNA_01806 0.62 -1.3 1,931,133 + CCNA_01806 0.63 -1.0 1,931,141 - CCNA_01806 0.64 -0.4 1,931,141 - CCNA_01806 0.64 +0.7 1,931,164 + CCNA_01806 0.65 +0.7
Or see this region's nucleotide sequence