Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01789 and CCNA_01790 are separated by 139 nucleotides CCNA_01790 and CCNA_01791 are separated by 115 nucleotides CCNA_01791 and CCNA_01792 are separated by 114 nucleotides CCNA_01792 and CCNA_01793 are separated by 123 nucleotides
CCNA_01789: CCNA_01789 - acetyltransferase, GNAT family, at 1,916,649 to 1,917,164
_01789
CCNA_01790: CCNA_01790 - protein translocase subunit secG, at 1,917,304 to 1,917,771
_01790
CCNA_01791: CCNA_01791 - CTP synthase, at 1,917,887 to 1,919,539
_01791
CCNA_01792: CCNA_01792 - LSU ribosomal protein L32P, at 1,919,654 to 1,919,836
_01792
CCNA_01793: CCNA_01793 - aspartate protease, at 1,919,960 to 1,920,958
_01793
Position (kb)
1917
1918
1919
1920 Strain fitness (log2 ratio)
-1
0
1
2 at 1916.897 kb on - strand, within CCNA_01789 at 1916.946 kb on - strand, within CCNA_01789 at 1916.954 kb on - strand, within CCNA_01789 at 1916.971 kb on + strand, within CCNA_01789 at 1916.971 kb on + strand, within CCNA_01789 at 1917.053 kb on + strand, within CCNA_01789 at 1917.061 kb on - strand, within CCNA_01789 at 1917.061 kb on - strand, within CCNA_01789 at 1917.072 kb on - strand, within CCNA_01789 at 1917.073 kb on - strand, within CCNA_01789 at 1917.102 kb on + strand, within CCNA_01789 at 1917.151 kb on + strand at 1917.153 kb on + strand at 1917.168 kb on + strand at 1917.180 kb on + strand at 1917.180 kb on + strand at 1917.180 kb on + strand at 1917.188 kb on - strand at 1917.208 kb on + strand at 1917.211 kb on - strand at 1917.570 kb on + strand, within CCNA_01790 at 1917.580 kb on + strand, within CCNA_01790 at 1917.675 kb on + strand, within CCNA_01790 at 1917.683 kb on - strand, within CCNA_01790 at 1917.759 kb on + strand at 1917.759 kb on + strand at 1917.759 kb on + strand at 1917.759 kb on + strand at 1917.765 kb on + strand at 1917.765 kb on + strand at 1917.772 kb on - strand at 1919.958 kb on - strand at 1919.987 kb on + strand at 1919.988 kb on - strand at 1920.050 kb on + strand at 1920.079 kb on + strand, within CCNA_01793 at 1920.218 kb on + strand, within CCNA_01793 at 1920.225 kb on + strand, within CCNA_01793 at 1920.226 kb on - strand, within CCNA_01793 at 1920.233 kb on - strand, within CCNA_01793 at 1920.233 kb on - strand, within CCNA_01793 at 1920.233 kb on - strand, within CCNA_01793 at 1920.233 kb on - strand, within CCNA_01793 at 1920.244 kb on + strand, within CCNA_01793 at 1920.252 kb on - strand, within CCNA_01793 at 1920.311 kb on + strand, within CCNA_01793 at 1920.347 kb on - strand, within CCNA_01793 at 1920.496 kb on + strand, within CCNA_01793
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 1,916,897 - CCNA_01789 0.48 +0.0 1,916,946 - CCNA_01789 0.58 +0.8 1,916,954 - CCNA_01789 0.59 +0.1 1,916,971 + CCNA_01789 0.62 +0.4 1,916,971 + CCNA_01789 0.62 -1.0 1,917,053 + CCNA_01789 0.78 +0.1 1,917,061 - CCNA_01789 0.80 +0.0 1,917,061 - CCNA_01789 0.80 -1.3 1,917,072 - CCNA_01789 0.82 -0.9 1,917,073 - CCNA_01789 0.82 +0.6 1,917,102 + CCNA_01789 0.88 -0.4 1,917,151 + +0.4 1,917,153 + +0.1 1,917,168 + +1.5 1,917,180 + -0.9 1,917,180 + -0.4 1,917,180 + +1.6 1,917,188 - +0.2 1,917,208 + -0.1 1,917,211 - -0.4 1,917,570 + CCNA_01790 0.57 +0.9 1,917,580 + CCNA_01790 0.59 +2.0 1,917,675 + CCNA_01790 0.79 +0.5 1,917,683 - CCNA_01790 0.81 +1.2 1,917,759 + +0.8 1,917,759 + +0.4 1,917,759 + +1.0 1,917,759 + -0.7 1,917,765 + -0.6 1,917,765 + +0.8 1,917,772 - -1.5 1,919,958 - -1.4 1,919,987 + -0.0 1,919,988 - +0.7 1,920,050 + -0.6 1,920,079 + CCNA_01793 0.12 +0.5 1,920,218 + CCNA_01793 0.26 +1.2 1,920,225 + CCNA_01793 0.27 +0.4 1,920,226 - CCNA_01793 0.27 +1.3 1,920,233 - CCNA_01793 0.27 +0.3 1,920,233 - CCNA_01793 0.27 -1.4 1,920,233 - CCNA_01793 0.27 -0.3 1,920,233 - CCNA_01793 0.27 -0.4 1,920,244 + CCNA_01793 0.28 +0.2 1,920,252 - CCNA_01793 0.29 -0.6 1,920,311 + CCNA_01793 0.35 +0.1 1,920,347 - CCNA_01793 0.39 -0.1 1,920,496 + CCNA_01793 0.54 +0.3
Or see this region's nucleotide sequence