Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01762 and CCNA_01763 are separated by 227 nucleotides CCNA_01763 and CCNA_01764 are separated by 120 nucleotides CCNA_01764 and CCNA_01765 overlap by 4 nucleotides CCNA_01765 and CCNA_01766 are separated by 42 nucleotides
CCNA_01762: CCNA_01762 - YjgP/YjgQ family membrane permease, at 1,890,632 to 1,891,771
_01762
CCNA_01763: CCNA_01763 - multifunctional aminopeptidase, at 1,891,999 to 1,893,480
_01763
CCNA_01764: CCNA_01764 - DNA polymerase III, chi subunit, at 1,893,601 to 1,894,062
_01764
CCNA_01765: CCNA_01765 - peptidase inhibitor, I78 family, at 1,894,059 to 1,894,400
_01765
CCNA_01766: CCNA_01766 - CsgG-related curli protein, at 1,894,443 to 1,895,228
_01766
Position (kb)
1891
1892
1893
1894 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1891.794 kb on - strand at 1891.834 kb on - strand at 1891.834 kb on - strand at 1891.834 kb on - strand at 1891.838 kb on + strand at 1891.840 kb on + strand at 1891.846 kb on - strand at 1891.847 kb on - strand at 1891.914 kb on + strand at 1891.914 kb on + strand at 1891.914 kb on + strand at 1891.914 kb on + strand at 1891.919 kb on + strand at 1891.922 kb on - strand at 1891.922 kb on - strand at 1891.927 kb on - strand at 1891.967 kb on + strand at 1892.039 kb on - strand at 1892.213 kb on + strand, within CCNA_01763 at 1892.231 kb on - strand, within CCNA_01763 at 1892.439 kb on + strand, within CCNA_01763 at 1892.448 kb on + strand, within CCNA_01763 at 1892.521 kb on + strand, within CCNA_01763 at 1892.524 kb on - strand, within CCNA_01763 at 1892.531 kb on + strand, within CCNA_01763 at 1892.546 kb on - strand, within CCNA_01763 at 1892.597 kb on - strand, within CCNA_01763 at 1892.655 kb on + strand, within CCNA_01763 at 1892.666 kb on + strand, within CCNA_01763 at 1892.805 kb on - strand, within CCNA_01763 at 1892.877 kb on + strand, within CCNA_01763 at 1892.901 kb on + strand, within CCNA_01763 at 1892.923 kb on + strand, within CCNA_01763 at 1892.929 kb on + strand, within CCNA_01763 at 1892.997 kb on - strand, within CCNA_01763 at 1893.034 kb on + strand, within CCNA_01763 at 1893.089 kb on - strand, within CCNA_01763 at 1893.292 kb on + strand, within CCNA_01763 at 1893.325 kb on + strand, within CCNA_01763 at 1893.426 kb on + strand at 1893.426 kb on + strand at 1893.426 kb on + strand at 1893.426 kb on + strand at 1893.426 kb on + strand at 1893.428 kb on + strand at 1893.438 kb on + strand at 1893.447 kb on - strand at 1893.461 kb on - strand at 1893.465 kb on + strand at 1893.479 kb on + strand at 1893.487 kb on - strand at 1893.502 kb on + strand at 1893.515 kb on - strand at 1893.515 kb on - strand at 1893.530 kb on - strand at 1893.530 kb on - strand at 1893.564 kb on - strand at 1893.612 kb on + strand at 1893.620 kb on - strand at 1893.620 kb on - strand at 1894.077 kb on - strand at 1894.085 kb on - strand at 1894.085 kb on - strand at 1894.085 kb on - strand at 1894.219 kb on + strand, within CCNA_01765 at 1894.258 kb on - strand, within CCNA_01765 at 1894.406 kb on + strand at 1894.422 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 1,891,794 - +0.5 1,891,834 - -2.5 1,891,834 - -0.4 1,891,834 - -1.2 1,891,838 + -0.6 1,891,840 + +1.1 1,891,846 - -0.0 1,891,847 - +0.3 1,891,914 + +1.2 1,891,914 + +0.1 1,891,914 + +0.3 1,891,914 + -0.3 1,891,919 + +0.4 1,891,922 - +1.0 1,891,922 - +0.0 1,891,927 - -1.6 1,891,967 + +1.0 1,892,039 - -0.7 1,892,213 + CCNA_01763 0.14 +0.7 1,892,231 - CCNA_01763 0.16 +0.3 1,892,439 + CCNA_01763 0.30 +0.8 1,892,448 + CCNA_01763 0.30 +0.7 1,892,521 + CCNA_01763 0.35 +1.1 1,892,524 - CCNA_01763 0.35 +0.3 1,892,531 + CCNA_01763 0.36 +2.2 1,892,546 - CCNA_01763 0.37 +0.0 1,892,597 - CCNA_01763 0.40 -1.9 1,892,655 + CCNA_01763 0.44 +0.0 1,892,666 + CCNA_01763 0.45 +0.5 1,892,805 - CCNA_01763 0.54 -0.1 1,892,877 + CCNA_01763 0.59 +1.5 1,892,901 + CCNA_01763 0.61 -0.8 1,892,923 + CCNA_01763 0.62 +1.1 1,892,929 + CCNA_01763 0.63 +0.1 1,892,997 - CCNA_01763 0.67 +0.1 1,893,034 + CCNA_01763 0.70 +0.1 1,893,089 - CCNA_01763 0.74 +2.1 1,893,292 + CCNA_01763 0.87 +0.4 1,893,325 + CCNA_01763 0.89 +1.2 1,893,426 + -0.1 1,893,426 + +1.9 1,893,426 + -1.0 1,893,426 + -0.0 1,893,426 + -1.0 1,893,428 + +0.6 1,893,438 + +0.4 1,893,447 - -0.3 1,893,461 - -0.8 1,893,465 + +0.6 1,893,479 + +0.5 1,893,487 - -0.2 1,893,502 + -0.2 1,893,515 - +0.5 1,893,515 - +0.0 1,893,530 - +0.4 1,893,530 - +0.5 1,893,564 - +0.9 1,893,612 + +0.5 1,893,620 - -2.3 1,893,620 - +0.2 1,894,077 - +0.6 1,894,085 - -0.6 1,894,085 - -1.6 1,894,085 - +0.8 1,894,219 + CCNA_01765 0.47 +0.7 1,894,258 - CCNA_01765 0.58 +1.0 1,894,406 + +0.6 1,894,422 + +0.7
Or see this region's nucleotide sequence