Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01726 and CCNA_01727 are separated by 78 nucleotides CCNA_01727 and CCNA_01728 are separated by 185 nucleotides CCNA_01728 and CCNA_01729 are separated by 48 nucleotides
CCNA_01726: CCNA_01726 - EngA-subfamily GTPase protein, at 1,852,884 to 1,854,650
_01726
CCNA_01727: CCNA_01727 - methyl-accepting chemotaxis protein, at 1,854,729 to 1,856,234
_01727
CCNA_01728: CCNA_01728 - short chain dehydrogenase, at 1,856,420 to 1,857,175
_01728
CCNA_01729: CCNA_01729 - transporter, sodium/bile acid symporter family, at 1,857,224 to 1,858,237
_01729
Position (kb)
1854
1855
1856
1857 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1854.314 kb on + strand, within CCNA_01726 at 1854.314 kb on + strand, within CCNA_01726 at 1854.543 kb on - strand at 1854.620 kb on + strand at 1854.644 kb on + strand at 1854.644 kb on + strand at 1854.644 kb on + strand at 1854.793 kb on + strand at 1854.839 kb on + strand at 1854.870 kb on + strand at 1854.880 kb on + strand, within CCNA_01727 at 1854.927 kb on + strand, within CCNA_01727 at 1854.943 kb on + strand, within CCNA_01727 at 1854.977 kb on + strand, within CCNA_01727 at 1855.014 kb on - strand, within CCNA_01727 at 1855.024 kb on + strand, within CCNA_01727 at 1855.025 kb on - strand, within CCNA_01727 at 1855.107 kb on + strand, within CCNA_01727 at 1855.109 kb on - strand, within CCNA_01727 at 1855.115 kb on - strand, within CCNA_01727 at 1855.149 kb on - strand, within CCNA_01727 at 1855.212 kb on - strand, within CCNA_01727 at 1855.234 kb on - strand, within CCNA_01727 at 1855.421 kb on - strand, within CCNA_01727 at 1855.431 kb on + strand, within CCNA_01727 at 1855.498 kb on - strand, within CCNA_01727 at 1855.528 kb on - strand, within CCNA_01727 at 1855.546 kb on - strand, within CCNA_01727 at 1855.578 kb on + strand, within CCNA_01727 at 1855.645 kb on - strand, within CCNA_01727 at 1855.647 kb on + strand, within CCNA_01727 at 1855.680 kb on + strand, within CCNA_01727 at 1855.776 kb on - strand, within CCNA_01727 at 1855.781 kb on + strand, within CCNA_01727 at 1855.861 kb on + strand, within CCNA_01727 at 1855.869 kb on - strand, within CCNA_01727 at 1855.894 kb on + strand, within CCNA_01727 at 1855.902 kb on - strand, within CCNA_01727 at 1855.973 kb on + strand, within CCNA_01727 at 1855.981 kb on - strand, within CCNA_01727 at 1855.982 kb on - strand, within CCNA_01727 at 1855.984 kb on + strand, within CCNA_01727 at 1856.044 kb on - strand, within CCNA_01727 at 1856.268 kb on + strand at 1856.374 kb on + strand at 1856.382 kb on - strand at 1856.388 kb on - strand at 1856.392 kb on - strand at 1856.472 kb on + strand at 1856.508 kb on + strand, within CCNA_01728 at 1856.508 kb on + strand, within CCNA_01728 at 1856.516 kb on - strand, within CCNA_01728 at 1856.518 kb on + strand, within CCNA_01728 at 1856.709 kb on + strand, within CCNA_01728 at 1856.717 kb on - strand, within CCNA_01728 at 1856.848 kb on + strand, within CCNA_01728 at 1856.872 kb on - strand, within CCNA_01728 at 1856.953 kb on - strand, within CCNA_01728 at 1856.976 kb on - strand, within CCNA_01728 at 1857.075 kb on + strand, within CCNA_01728 at 1857.094 kb on - strand, within CCNA_01728 at 1857.128 kb on + strand at 1857.154 kb on + strand at 1857.154 kb on + strand at 1857.164 kb on + strand at 1857.183 kb on + strand at 1857.191 kb on - strand at 1857.222 kb on + strand at 1857.229 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 1,854,314 + CCNA_01726 0.81 -0.3 1,854,314 + CCNA_01726 0.81 -1.5 1,854,543 - +1.4 1,854,620 + -0.7 1,854,644 + +0.6 1,854,644 + +0.7 1,854,644 + +0.2 1,854,793 + -1.2 1,854,839 + +0.3 1,854,870 + +0.4 1,854,880 + CCNA_01727 0.10 +0.7 1,854,927 + CCNA_01727 0.13 +0.2 1,854,943 + CCNA_01727 0.14 -0.5 1,854,977 + CCNA_01727 0.16 +1.3 1,855,014 - CCNA_01727 0.19 -0.3 1,855,024 + CCNA_01727 0.20 +0.4 1,855,025 - CCNA_01727 0.20 +1.3 1,855,107 + CCNA_01727 0.25 +0.1 1,855,109 - CCNA_01727 0.25 +1.0 1,855,115 - CCNA_01727 0.26 +1.6 1,855,149 - CCNA_01727 0.28 +0.8 1,855,212 - CCNA_01727 0.32 -0.8 1,855,234 - CCNA_01727 0.34 +0.8 1,855,421 - CCNA_01727 0.46 +0.5 1,855,431 + CCNA_01727 0.47 +0.3 1,855,498 - CCNA_01727 0.51 +1.3 1,855,528 - CCNA_01727 0.53 +0.2 1,855,546 - CCNA_01727 0.54 +0.2 1,855,578 + CCNA_01727 0.56 +0.2 1,855,645 - CCNA_01727 0.61 +1.3 1,855,647 + CCNA_01727 0.61 -0.3 1,855,680 + CCNA_01727 0.63 -0.2 1,855,776 - CCNA_01727 0.70 +0.5 1,855,781 + CCNA_01727 0.70 +0.5 1,855,861 + CCNA_01727 0.75 -0.1 1,855,869 - CCNA_01727 0.76 -0.2 1,855,894 + CCNA_01727 0.77 +0.2 1,855,902 - CCNA_01727 0.78 +3.1 1,855,973 + CCNA_01727 0.83 +0.2 1,855,981 - CCNA_01727 0.83 +0.8 1,855,982 - CCNA_01727 0.83 -1.6 1,855,984 + CCNA_01727 0.83 +0.2 1,856,044 - CCNA_01727 0.87 +0.6 1,856,268 + +0.5 1,856,374 + +0.1 1,856,382 - +0.4 1,856,388 - +1.1 1,856,392 - +0.5 1,856,472 + +0.3 1,856,508 + CCNA_01728 0.12 -0.4 1,856,508 + CCNA_01728 0.12 +0.5 1,856,516 - CCNA_01728 0.13 +0.6 1,856,518 + CCNA_01728 0.13 -2.4 1,856,709 + CCNA_01728 0.38 +0.2 1,856,717 - CCNA_01728 0.39 -0.4 1,856,848 + CCNA_01728 0.57 +0.2 1,856,872 - CCNA_01728 0.60 -0.6 1,856,953 - CCNA_01728 0.71 +0.4 1,856,976 - CCNA_01728 0.74 +0.2 1,857,075 + CCNA_01728 0.87 +0.2 1,857,094 - CCNA_01728 0.89 -0.4 1,857,128 + +0.1 1,857,154 + +1.1 1,857,154 + +0.6 1,857,164 + -1.2 1,857,183 + -1.0 1,857,191 - +0.4 1,857,222 + -1.4 1,857,229 - -0.0
Or see this region's nucleotide sequence